Authentication of Gemlik olive cultivar using 1H NMR spectroscopy and chemometric analysis

In this study, 1H NMR spectroscopy was used to discriminate Gemlik olives cultivated in a protected designation of origin(PDO) region from those cultivated in non-PDO regions. For this purpose, 95 Gemlik olive samples (47 from PDO and 48 from nonPDO regions) were collected. 1H NMR spectral analysis was carried out on the oils extracted from these olive samples. The results ofthe partial least squares-discriminant analysis showed that olives from the PDO and non-PDO regions could be discriminated onthe basis of their NMR profiles. The prediction ability of the model was better when olive samples from the district of Mudanya wereincluded in the PDO group. A detailed analysis of the NMR spectra revealed that PDO olives could be discriminated from non-PDOsamples mainly due to their higher terpene content. The outcomes of this study clearly indicated that NMR spectroscopy coupled withmultivariate statistical analysis has great potential to be used as an objective analytical measure for the discrimination of Gemlik olivescultivated in PDO and non-PDO regions.

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Alonso-Salces R, Segebarth N, Garmón-Lobato S, Holland M, Moreno-Rojas J, Fernández-Pierna J, Baeten V, Fuselli S, Gallo B, Berrueta Luis A et al. (2015). 1 H-NMR and isotopic fingerprinting of olive oil and its unsaponifiable fraction: geographical origin of virgin olive oils by pattern recognition. Eur J Lipid Sci Technol 117: 1991-2006.
Christophoridou S, Dais P (2009). Detection and quantification of phenolic compounds in olive oil by high resolution 1H nuclear magnetic resonance spectroscopy. Anal Chim Acta 633: 283- 292.
Clodoveo M, Camposeo S, Amirante R, Dugo G, Cicero N, Boskou D (2015). Research and innovative approaches to obtain virgin olive oils with a higher level of bioactive constituents. In: Boskou D (editor). Olive and Olive Oil Bioactive Constituents. 1st ed. Urbana, IL, USA: AOCS Press, pp. 179-215.
Dais P, Hatzakis E (2013). Quality assessment and authentication of virgin olive oil by NMR spectroscopy: A critical review. Anal Chim Acta 765: 1-27.
D’Imperio M, Mannina L, Capitani D, Bidet O, Rossi E, Bucarelli FM, Quaglia GB, Segre A (2007). NMR and statistical study of olive oils from Lazio: a geographical, ecological and agronomic characterization. Food Chem 105: 1256-1267.
Dokuzlu, S (2016). Geographical indications, implementation and traceability: Gemlik table olives. Br Food J 118: 2074-2085.
Fauhl C, Reniero F, Guillou C (2000). 1 H NMR as a tool for the analysis of mixtures of virgin olive oil with oils of different botanical origin. Magn Reson Chem 38: 436-443.
Kiralan M, Ozkan G, Koyluoglu F, Asik Ugurlu H, Bayrak A, Kiritsakis A (2012). Effect of cultivation area and climatic conditions on volatiles of virgin olive oil. Eur J Lipid Sci Technol 114: 552-557.
Lauri I, Pagano B, Malmendal A, Sacchi R, Randazzo A (2013). Application of “magnetic tongue” to the sensory evaluation of extra virgin olive oil. Food Chem 140: 692-699.
Longobardi F, Ventrella A, Casiello G, Sacco D, Tasioula-Margari M, Kiritsakis AK, Kontominas MG (2012). Characterisation of the geographical origin of Western Greek virgin olive oils based on instrumental and multivariate statistical analysis. Food Chem 133: 169-175.
Luykx DMAM, van Ruth SM (2008). An overview of analytical methods for determining the geographical origin of food products. Food Chem 107: 897-911.
Mannina L, Marini F, Gobbino M, Sobolev AP, Capitani D (2010). NMR and chemometrics in tracing European olive oils: the case study of Ligurian samples. Talanta 80: 2141-2148.
Meral Y, Şahin A (2013). Tüketicilerin coğrafi işaretli ürün algısı: Gemlik zeytini örneği. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilim Dergisi 16: 16-24 (in Turkish).
Özdemir İS, Dağ Ç, Makuc D, Ertaş E, Plavec J, Bekiroğlu S (2018). Characterisation of the Turkish and Slovenian extra virgin olive oils by chemometric analysis of the presaturation 1 H-NMR spectra. LWT Food Sci 92: 10-15.
Pehlivan Gürkan N (2015). Turkish olive and olive oil sectoral innovation system: A functional-structural analysis. PhD, Middle East Technical University, Ankara, Turkey. Sacchi R, Addeo F, Paolilo L (1998). 1 H and 13C NMR of virgin olive oil. An overview. Magn Reson Chem 35: 133-145.
Santos JS, Escher GB, da Silva Pereira JM, Marinho MT, do PradoSilva L, Sant’Ana AS, Dutra LM, Barison A, Granato D (2018). 1 H NMR combined with chemometrics tools for rapid characterization of edible oils and their biological properties. Ind Crops Prod 116: 191-200.
Valli E, Bendini A, Berardinelli A, Ragni L, Riccò B, Grossi M, Gallina Toschi T (2016). Rapid and innovative instrumental approaches for quality and authenticity of olive oils. Eur J Lipid Sci Technol 118: 1601-1619.
Xia J, Wishart DS (2016). Using MetaboAnalyst 3.0 for comprehensive metabolomics data analysis. Curr Protoc Bioinforma 10-14.