A new entomopathogenic nematode species for Turkey, Heterorhabditis megidis Poinar, Jackson & Klein 1987 (Rhabditida: Heterorhabditidae)

Türkiye’nin Doğu Karadeniz Bölgesi’nde entomopatojenik nematodların varlığını belirlemek için yapılan bir survey çalışması sırasında, Galleria tuzak böcek yöntemi kullanılarak bir heterorhabditid türü tespit edildi. Yapılan morfolojik ve morfometrik incelemeler sonucunda izole edilen türün Heterorhabditis megidis olduğu belirlendi. Bu identifikasyon, rRNA’nın ITS bölgesi sekansı ile doğrulandı. Bu çalışma ile Heterorhabditis megidis Türkiye’den ilk kez izole edilmiştir. Karadeniz Bölgesi’nin tamamını kapsayan detaylı survey çalışması halen yürütülmektedir.

Türkiye için yeni bir entomopatojenik nematod türü Heterorhabditis megidis Poinar, Jackson & Klein 1987 (Rhabditida: Heterorhabditidae)

During a survey on the occurrence of entomopathogenic nematodes (EPNs) in the Eastern Black Sea region of Turkey, a heterorhabditid species was isolated using the Galleria-baiting technique. Based on morphology and morphometrics, the isolate was identified as Heterorhabditis megidis. Sequences of the ITS region of its rDNA confirmed this identification. The species is recorded for the first time from Turkey. A more intensive survey to determine the distribution of this species, covering all parts of the Black Sea region of Turkey, is currently underway.

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  • Adams, B.J. and K.B. Nguyen. 2002. Taxonomy and Systematics. In: Entomopathogenic Nematology, (Ed.: R. Gaugler). CABI Publishing, Wallingford UK, pp 1-33.
  • Bedding, R.A. and R.J. Akhurst. 1975. A simple technique for detection of insect parasitic rhabditid nematodes in soil. Nematologica. 21: 109-110.
  • Bedding, R.A. 1990. Logistics and strategies for introducing entomopathogenic nematodes technology into developing countries. In: Entomopathogenic Nematodes in Biological Control, (Eds.: R. Gaugler and H.K. Kaya). CRC press, Florida, pp 233-245.
  • Boemare, N. 2002. Biology, Taxonomy and Systematics of Photorhabdus and Xenorhabdus. In: Entomopathogenic Nematology, (Ed.: R. Gaugler). CABI Publishing, Wallingford UK, pp 35-56.
  • Ciche, T.A and J.C. Ensign. 2003. For the insect pathogen, Photorhabdus luminescens, which end of a nematode is out? Appl. Environ. Microbiol. 69: 1890-1897.
  • De Grisse, A.T. 1969. Redescription ou modifications de quelques techniques utilisées dans l’étude de nématodes phytoparasitaires. Mededelingen Rijksfaculteit Landbouwwetenschappen Gent. 34: 315-359.
  • Del Pino, G.F. and A. Palomo. 1996. Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Spanish soils. J. Invertebr. Pathol. 68: 84-90.
  • Fischer-Le Saux, M., H. Mauleon, P. Constant, B. Brunel and N. Boemare. 1998. PCR-ribotyping of Xenorhabdus and Photorhabdus isolates from the Caribbean region in relation to the taxonomy and geographic distribution of their nematode hosts. Appl. Environ. Microbiol. 64: 4246-4254.
  • Glazer, I., N. Liran, G.O. Poinar and P.H. Smits. 1993. Identification and biological activity of newly isolated heterorhabditid populations from Israel. Fun. Appl. Nematol. 16: 467-472.
  • Griffin, C.T., I. Dix, S.A. Joyce, A.M. Burnell and M.J. Downes. 1999. Isolation and characterization of Heterorhabditis ssp. (Nematoda: Heterorhabditidae) from Hungary, Estonia and Denmark. Nematology. 1: 321-332.
  • Griffin, C.T., R. Chaerani, D. Fallon, A.P. Reid and M.J. Downes. 2000. Occurrence and distribution of the entomopathogenic nematodes Steinernema ssp. and Heterorhabditis indica in Indonesia. J. Helmintol. 74: 143-150.
  • Hara, A.H., R. Gaugler, H.K. Kaya and L.M. Lebeck. 1991. Natural population of entomopathogenic nematodes (Rhabditida: Heterorhabditidae, Steinernematidae) from the Hawaiian islands. Environ. Entomol. 20: 211-216.
  • Hazır, S., S.P. Stock and N. Keskin. 2003a. A new entomopathogenic nematode, Steinernema anatoliense n. sp. (Rhabditida: Steinernematidae), from Turkey. Syst. Parasitol. 55: 211-220.
  • Hazır, S., N. Keskin, P.S. Stock, H.K. Kaya and S. Özcan. 2003b. Diversity and distribution of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Turkey. Biodivers. Conserv. 12: 375-386.
  • Hominick, W.M. 2002. Biogeography. In: Entomopathogenic Nematology, (Ed.: R Gaugler), CABI Publishing, Wallingford, UK, pp. 115-143.
  • Joyce, S.A., A. Reid, F. Driver and J. Curran. 1994. Application of polymerase chain reaction (PCR) methods to identification of entomopathogenic nematodes. In: Biotechnology: Genetics of Entomopathogenic Nematode Bacteria Complexes, (Eds.: A.M. Burnell, R.U. Ehlers, J.P. Masson), European Commission, Luxembourg, DG XII, pp 178-187.
  • Kaya, H.K and R. Gaugler. 1993. Entomopathogenic nematodes. Ann. Rev. Entomol. 38: 181-206.
  • Kaya, H.K. and S.P. Stock. 1997. Techniques in Insect Nematology. In: Manual of Techniques in Insect Pathology, (Ed.: L.A. Lacey), Academic Press, London, pp 281-324.
  • Kepenekçi, İ., N.E. Babaroğlu, G. Öztürk and S. Halıcı. 1999. Türkiye için yeni bir entomopatojen nematod; Heterorhabditis bacteriophora Poinar, 1976 (Rhabditida: Heterorhabditidae). Türkiye Entomoloji Derneği Yayınları. 9: 587-596.
  • Kepenekçi, İ. and İ.A. Susurluk. 2000. Türkiye için yeni bir entomopatojen nematod türü; Heterorhabditis marelatus Lui and Berry, 1996 (Rhabditida: Heterorhabditidae). Tarım Bilimleri Dergisi. 6: 59-64.
  • Kepenekçi, İ. and İ.A. Susurluk. 2003. Three entomopathogenic nematodes (Rhabditida) from Turkey. Pakistan J. Nematol. 21: 19-23.
  • Kepenekçi, I. 2002. Entomopathogenic nematodes (Rhabditida) in the Mediterranean region of Turkey. Nematol. Mediter. 30: 13- 15.
  • Menti, H., D.J. Wright and R.N. Perry. 1997. Desiccation survival of populations of the entomopathogenic nematodes Steinernema feltiae and Heterorhabditis megidis from Greece and the UK. J. Helmintol. 71: 41-46.
  • Mracek, Z. and J.M. Webster. 1993. Survey of Heterorhabditidae and Steinernematidae (Rhabditida: Nematoda) in Western Canada. J. Nematol. 25: 710-717.
  • Özer, N., N. Keskin and Z. Kırbaş. 1995. Occurrence of entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) in Turkey. Nematologica. 41: 639-640.
  • Poinar, G.O., T. Jackson and M. Klein. 1987. Heterorhabditis megidis sp. n. (Rhabditida: Heterorhabditidae) parasitic in the Japanese betle, Popillia japonica (Scarabaeidae: Coleoptera), in Ohio. Proceedings of the Helminthological Society of Washington. 54: 53-59.
  • Rosa, J.S., E. Bonifassi, J. Amaral, L.A. Lacey, N. Simoes and C. Laumond. 2000. Natural occurrence of entomopathogenic nematodes (Rhabditida: Steinernema, Heterorhabditis) in the Azores. J. Nematol. 32: 215-222.
  • Seinhorst, J.W. 1959. A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica 4: 67-69.
  • Stock, P.S. and H.K. Kaya. 1996. A multivariate analysis of morphometric characters of Heterorhabditis species (Nematoda: Heterorhabditidae) and the role of morphometrics in the taxonomy of species of genus. J. Parasitol. 82: 806-813.
  • Stock, P.S., D.R. Strong and S.L. Gardner. 1996. Identification of Heterorhabditis (Nematoda: Heterorhabditidae) from California with a new species isolated from the larvae of the ghost moth Hepialis californicus (Lepidoptera: Hepialidae) from the Bodega Bay Natural Reserve. Fun. Appl. Nematol. 19: 585-592.
  • Stuart, R.J. and R. Gaugler. 1994. Patchiness in populations of entomopathogenic nematodes. J. Invertebr. Pathol. 64: 39-45.
  • Susurluk, A., I. Dix, E. Stackebrandt, O. Strauch, U. Wyss and R.U. Ehlers. 2001. Identification and ecological characterization of three entomopathogenic nematode-bacterium complexes from Turkey. Nematology. 3: 833-841.
  • Susurluk, A., S. Hollmer, U.K. Mehta, R. Han, E. Tarasco, O. Triggiani, A. Peters, R.U. Ehlers. 2003. Molecular identification of entomopathogenic nematodes from Turkey, India, China, Italy, Norway, Albania and Germany by PCR-RFLP. In: 9th European Meeting of the IOBC/WPRS Working Group, Schloss Salzau, Germany.
  • Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. Clustal W: improving the sensitivity of progressive multiple sequence alignment though sequence weighting, position gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680.
  • Ünlü, I.O., R.U. Ehlers and A. Susurluk. 2007. Additional data and first record of entomopathogenic nematode Steinernema weiseri from Turkey. Nematology. 9: 739-741.
  • Waturu, C.N. 1998. Entomopathogenic nematodes (Steinernematidae and Heterorhabditidae) from Kenya. PhD thesis, University of Reading, UK, p. 191.
  • White, G.F. 1927. A method for obtaining infecting nematode larvae from culture. Science. 66: 302-303.
  • Yoshida, M., A.P. Reid, B.R. Briscoe and W.M. Hominick. 1998. Survey of entomopathogenic nematodes (Rhabditida: Steinernematidae and Heterorhabditidae) in Japan. Fun. Appl. Nematol. 21: 185-198.
Turkish Journal of Agriculture and Forestry-Cover
  • ISSN: 1300-011X
  • Yayın Aralığı: Yılda 6 Sayı
  • Yayıncı: TÜBİTAK
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