Gulsemin Savas TUNA, Gulru YUCEL, Tansel Kaygisiz ASCİOGUL, Duygu ATES, Dursun ESİYOK, Muhammed Bahattin TANYOLAC, Metin TUNA

Molecular cytogenetic characterization of common bean (Phaseolus vulgaris L.) accessions

With an annual global production of approximately 25 million tons, the common bean (Phaseolus vulgaris L), a member of the genus Phaseolus, is one of the major protein sources used as food for humans. In this study, it was aimed to investigate the genome size of the common bean genetic resource collection (154 common bean accessions) in Turkey by flow cytometry (FCM) and determine whether geographical variables affected the genome size. In addition, the number and distribution of 5S and 45S ribosomal DNA loci were designated by performing a fluorescence in situ hybridization (FISH) analysis in some of the accessions. The FCM analyses revealed that the mean nuclear DNA content of the accessions varied from 1.28 pg2C-1 to 1.55 pg2G1 (mean 1.35 pg2C-1), and the differences between these accessions were statistically significant (P

Keywords:

Phaseolus vulgaris, genome size, flow cytometry, geographical variables, fluorescence in situ hybridization, gene pool,

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