Ece TURHAN, Cigdem AYDOGAN, Sergul ERGIN, Nilufer OZTURK

Variation in Heat Stress-Induced Some Physiological Changes and Peroxidase Activities Among Three Tomato (Lycopersicon esculentum Mill.) Cultivars

Çaltı, Pembe ve Yaren domates (Lycopersicon esculentum Mill.) çeşitlerinde sıcaklık stresinin teşvik ettiği bazı fizyolojik değişiklikler ve peroksidaz (POX) aktivitelerindeki farklılıklar belirlenmiştir. Bu amaçla domates bitkilerinden ilk çiçeklenme ve verim dönemlerinde yaprak örnekleri toplanarak, su banyosunda sıcaklık kademeli olarak arttırılarak 35, 40, 45, 50, 55 ve 60 °C sıcaklıklarda, her sıcaklık kademesinde 30 dakika tutulmuştur. Yaprak örneklerinde iyon sızıntısı, turgor kaybı, çözünebilir peroksidaz (S-POX) ve hücre duvarı peroksidaz (CWB-POX) aktiviteleri belirlenmiştir. Genel olarak sıcaklık stresinin incelenen parametreler üzerindeki etkileri önemli bulunmuştur. Sonuçlar, iyon sızıntısı ve turgor kaybı değerlerinin sıcaklıklara paralel olarak arttığını göstermiştir. Bunun yanı sıra turgor kaybı değerleri verim döneminde ilk çiçeklenme dönemine göre daha yüksek bulunmuştur. POX aktiviteleri değerlendirildiğinde, S-POX aktivitesinin tüm çeşitlerde ilk çiçeklenme döneminde verim dönemine oranla daha yüksek olduğu tespit edilmiştir. Diğer taraftan en yüksek ve en düşük S-POX aktivitesi sırasıyla Yaren ve Pembe çeşitlerinde olmuştur. CWB-POX aktivitesi ise S-POX aktivitesinin aksine verim döneminde ilk çiçeklenme dönemine oranla daha yüksek bulunmuştur. En yüksek CWB-POX aktivitesinin Çaltı, en düşük CWB-POX aktivitesinin ise Pembe çeşidinde olduğu saptanmıştır. Veriler aynı zamanda CWB-POX aktivitesinin genellikle sıcaklık stresi uygulamasına bağlı olarak arttığını göstermiştir

Anahtar Kelimeler:

Domates, sıcaklık stresi, iyon sızıntısı, turgor kaybı, peroksidaz

Variation in Heat Stress-Induced Some Physiological Changes and Peroxidase Activities Among Three Tomato (Lycopersicon esculentum Mill.) Cultivars

Variation in heat stress-induced some physiological changes and peroxidase (POX) activities were studied in three tomato (Lycopersicon esculentum Mill.) cultivars, Çaltı, Pembe and Yaren. For this purpose, the leaves were collected from tomato plants at the first bloom and yield stages. The leaves were subjected to heat stress treatments in water bath at 35, 40, 45, 50, 55 and 60 °C with gradual increments every half an hour. The leaves were then analysed for ion leakage, loss of turgidity, soluble peroxidase (S-POX) and cell wall-bound peroxidase (CWB-POX) activities. In general, effects of heat stress on the variables studied were significant. Results revealed that ion leakage and loss of turgidity were increased parallel to the temperatures. In addition, loss of turgidity values were higher in the yield stage than those in the first bloom stage. Considering the POX activities, the SPOX activity was greater in the first bloom stage than in the yield stage in all cultivars. Moreover, the highest and the lowest enzyme activity were detected in cvs. Yaren and Pembe, respectively. In contrast to S-POX activity, the CWB-POX activity was greater in the yield stage than in the first bloom stage in cvs. Çaltı and Pembe. The highest CWB-POX activity was detected in cv. Çaltı while the lowest activity was detected in cv. Pembe. Data also indicated that, generally CWB-POX activity was high in the samples in response to heat stress treatments

Keywords:

Tomato, heat stress, ion leakage, loss of turgidity, peroxidase,

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Anderson, J.A. 2002. Catalase activity, hydrogen peroxide content and thermotolerance of pepper leaves, Scientia Hort., 95: 277–284.
Andrews J., Malone, M., Thompson, D.S., Ho, L.C., Burton, K.S. 2000. Peroxidase isoenzyme patterns in the skin of maturing tomato fruits. Plant Cell Environ 23:415-422.
Arora, R., Wisniewski, M.E., Scorza, R. 1992. Cold acclimation in genetically related (sibling) deciduous and evergreen peach (Prunus persica [L.] Batsch) I: Seasonal changes in cold-hardiness and polypeptides of bark and xylem tissues. Plant Physiol 99:1562-1568.
Arora, R., Pitchay, D. S. and Bearce, B. C. 1998. Water-stress induced heat tolerance in geranium leaf tissues: A possible linkage through Plantarum, 103: 24-34. Physiologia uygulamasının keklik barbunya çeşidinin bazı fizyolojik özelliklerine etkisi. İç Anadolu Bölgesi 1. Tarım ve Gıda Kongresi, Bildiriler, Cilt I, Bitkisel Üretim, s:278-283.
Barr, H.D. and Weatherley, P.E., 1962. A re- examination of the relative turgidity technique for estimating water deficit in leaves. Aust. J. Biol.Sci., 15: 413-428.
Benkeblia, N. and Shiomi, N. 2004. Chilling effect on the soluble sugars, respiration rate, total phenolics, peroxidase activity and dormancy of onion bulbs. Scientia Agricola 61:281-285.
Cansev, A. 2012. Physiological effects of high temperature treatments on leaves of olive cv. Gemlik. Plant Archives., 12(1):521-525.
Cansev, A. and Kesici, M. 2013. Changes in antioxidant enzyme activities during cold- acclimation in sweet cherry cultivars grafted on different rootstocks. Journal of Food, Agriculture & Environment Vol.11 (1): 522- 527.
Chaitanya, K.V., Sundar, D., Masilamani, S., Reddy, A.R. 2002. Variation in heat stress-induced antioxidant enzyme activities among three mulberry cultivars. Plant Growth Regul., 36: 175-180.
Chen, T.H.H., Shen, Z.Y., Lee, P.H., 1982. Adaptability of crop plants to high temperature stress. Crop Sci., 22: 719–725.
Else, M., Atkinson, C. 2010. Climate change impacts on UK top and soft fruit production. Outlook Agr 39:257-262.
Gaspar, T.H., Penel, C.L., Thorpe, T. and Grappin, H. 1982. Peroxidases a survey of their biochemical and physiological roles in higher plants. Unıversıté De Genève Press., Genève. pp. 10-60.
Gulen, H. and Eris, A. 2003. Some physiological changes in Ananassa cv. ‘Camarosa’) plants under heat stress. Journal of Horticultural Science & Biotechnology, 78: 894-898. (Fragaria ×
Gulen, H. and Eris, A. 2004. Effect of heat stress on peroxidase activity and total protein content in strawberry plants. Plant Science, 166: 739- 744.
Gupta N.K., Agarwal, S., Agarwal, V.P., Nathawat N.S., Gupta, S., Singh, G. 2013. Effect of short-term physiology system in wheat seedlings. Acta Physiol Plant, 35:1837–1842. on growth, defence
Hasanuzzaman, M., Nahar, K., Alam, Md. M., R., Roychowdhury, Physiological, Biochemical, and Molecular M. 2013. Mechanisms of Heat Stress Tolerance in Plants. Int. J. Mol. Sci. 14: 9643-9684.
Kesici, M., Gulen, H., Ergin, S., Turhan, E., Ipek, A. and Koksal, N. 2013. Heat-stress tolerance of some strawberry (Fragaria × ananassa) cultivars. Agrobotanici Cluj-Napoca, 41(1):238-243. Horti
Kumar, S., Sairam, R.K., Prabhu, K.V. 2013. Physiological traits for high temperature stress tolerance in Brassica juncea. Indian Journal of Plant Physiology, 18(1): 89-93. Responses Levitt, J. 1980. of plants to environmental stresses. Vol I., Academic Press, London and New York.
Mansour, A., Ismail, H.M. Ramadan, M.F. Gyulai, G. 2009. Variations in tomato (Lycopersicon esculentum) cultivars grown under heat stress. Journal für Verbraucherschutz und Lebensmittelsicherheit, 4:118-127.
Mazorra, L.M., Nunez, M., Hechavarria, M., Coll, F. Sanchez-Blanco, M.J. 2002. Influence of brassinosteroids on antioxidant enzymes activity in temperatures, Biol. Plant., 45: 593-596.
Mika, A., Boenisch, M.J., Hopff, D., Lüthje, S. 2010. Membrane-bound guaiacol peroxidases are regulated by methyl jasmonate, salicylic acid, and pathogen elicitors. J. Exp. Bot. 61: 831–841.
Prasad, T. K., Anderson, M. D. and Stewart, C. R. 1995. Localization and characterization of peroxidases in the mitochondria of chilling- acclimated maize seedlings. Plant Physiol. 108:1597–1605.
Ros-Barceló, A., Muñoz, R. and Sabater, F. 1988. Lupin peroxidases III. Subcellular location of membrane-bound acidic isoperoxidases. Plant Physiol. Biochem. 26:575–583.
Verma K, Shekhawat GS, Sharma A, Mehta SK, Sharma V. 2008. Cadmium induced oxidative stress and changes in soluble and ionically bound cell wall peroxidase activities in roots of seedling and 3-4 leaf stage plants of Brassica juncea (L.) czern. Plant Cell Rep. 2008 Jul;27(7):1261-9.
Vicuna, D. 2005. The role of peroxidases in the development of plants and their responses to abiotic stresses. Doctoral Thesis. Paper 15. http://arrow.dit.ie/sciendoc/15.
Wahid, A., Gelani, S., Ashraf, M., Foolad, M.R. 2007. Heat tolerance in plants: An overview. Environmental and Experimental Botany 61: 199–223.
Wang, Y. Wisniewski, M., Meilan, R., Cui, M. and Fuchigami, L. 2006. Transgenic tomato (Lycopersicon esculentum) overexpressing cAPX exhibits enhanced tolerance to UV-B and heat stress. Journal of Applied Horticulture, 8(2): 87-90.
Yin, H., Chen, Q. Yi, M. 2008. Effects of short-term heat stress on oxidative damage and responses of antioxidant system in Lilium longiflorum. Plant Growth Regulation, 54 (1): 45–54.