Rezan A. BAYAT, Şebnem KUŞVURAN, Şebnem ELLİALTIOĞLU, A. Sülün ÜSTÜN

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Tuz Stresi Altındaki Genç Kabak (Cucurbita pepo L. ve C. moschata Poir.) Bitkilerine Uygulanan Prolin’in, Antioksidatif Enzim Aktiviteleri Üzerine Etkisi

Prolin, NaCl stresi altındaki bitkilerde protein bütünlüğünün sağlanması ve enzimlerin aktive edilmesinde görev yapan önemli bir ozmoregülatördür. Stres faktörleri, bitkilerdeki içsel prolin miktarında artışa neden olmaktadır. Dışsal prolin uygulamalarının da tuz stresine toleransta olumlu etki yaptığı bilinmektedir. Burada sunulan çalışmada tuza tolerans seviyeleri önceden belirlenmiş, birisi orta düzeyde tolerant (A-19, C. moschata Poir.), diğeri duyarlı (Ç-1, C. pepo L.) olan iki yerel kabak çeşidine yapılan dışsal prolin uygulamasının, bitkilerdeki antioksidatif enzimlerin seviyesi üzerine etkisi araştırılmıştır. Kabak tohumları vermikulit ortamında çimlendirilmiş ve 2 gerçek yapraklı aşamada hidroponik kültüre aktarılmıştır. Bir hafta sonra bitkiler ‘Kontrol’ ‘100 mM tuz (NaCl)’, ‘100 mM tuz (NaCl) + 5 mM prolin’ ve ‘100 mM tuz (NaCl) + 10 mM prolin’ olmak üzere 4 farklı uygulamaya tabi tutulmuştur. Stresin 10. gününde sürgünün ucundan geriye doğru 2-4. yapraklardan hazırlanan örnekler, süperoksit dismutaz (SOD), katalaz (CAT), askorbat peroksidaz (APX) ve glutayon redüktaz (GR) enzim aktiviteleri bakımından değerlendirilmiştir. Genel olarak prolin uygulamaları, kabak yapraklarının enzim aktivitelerinde kontrole göre artışa neden olmuştur. Bu etki, tuza toleransı daha yüksek olan A-19 genotipinde daha belirgin olmuştur. Dışsal prolin uygulamasının kabak bitkisinde tuza toleransın artmasında olumlu etki yaptığı, bu etkiyi antioksidatif enzim sistemini harekete geçirerek pekiştirdiği izlenimi edinilmiştir.
Anahtar Kelimeler:

Cucurbita pepo, C. moschata, Tuzluluk, Prolin, SOD, CAT, GR, APX

Effects of Proline Application on Antioxidative Enzymes Activities in the Young Pumpkin Plants (Cucurbita pepo L. and C. moschata Poir.) under Salt Stress

Proline is known to play an important role as an osmolyte which maintains of integrity proteins and activated of antioxidative enzymes in plants subjected to NaCl stress. The stress factors are caused increasing to internal proline amount in plants. Exogenous proline application has been known to positive effect in salt tolerance. The effect of exogenous proline application on antioxidative enzymes activity was investigated in this study, by using the semi-salt-tolerant (A-19, C. moschata Poir.) and the salt-sensitive (Ç-1, C. pepo L.) pumpkin genotypes. Pumpkin seeds were germinated in vermiculite and the seedlings at two true-leaf stage were transferred to hydroponic culture (Hoagland solution). One week after transplanting the plants were subjected to four different applications (Control, 100 mM salt (NaCl), 100 mM salt (NaCl) + 5 mM proline, and 100 mM salt (NaCl) + 10 mM proline). Superoxide dismutase (SOD), catalaz (CAT), ascorbate peroxidase (APX) and glutathione reductase (GR) enzymes activity measurements and evaluations have been completed after 10 days of salt application. Antioxidant enzymes activities increased in the salt- and proline-treated plants very effectively compared to control plants. However, this effect was clearer in the A-19 salt tolerance genotype than the C-1 sensitive genotype. Exogenous proline was found useful in protecting the adverse effects of salt stress on plants and this effect may be responded with enzymatic defense systems against salt induced oxidative stress.
Keywords:

Cucurbita pepo, C. moschata, Salinity, Proline, SOD, CAT, GR, APX,

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Türk Tarım ve Doğa Bilimleri Dergisi-Cover
  • ISSN: 2148-3647
  • Yayın Aralığı: Yılda 4 Sayı
  • Başlangıç: 2014
  • Yayıncı: Prof. Dr. Mevlüt AKÇURA
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