Alparslan KOÇAK, Gülden KOÇAK, İlyas DENİZ

Türkiye’ye Ait Bazı Geofit Taksonlarının Moleküler Filogenisi, Sistematik Yaklaşımlar

Bu çalışmada Kulp-Diyarbakır bölgesindeki doğal habitatlarından toplanan geofitlerin bazı üyeleri arasındaki evrimsel ilişkileri belirlemek için nükleer DNA (nrDNA) bölgesi (internal transkribed spacer, ITS) ve kloroplast DNA(cpDNA) bölgesinin (intergenic spacer, trnL-F) moleküler filogenetik analizi gerçekleştirdik. İki farklı DNA kaynağı kullanılarak yapılan maximum likelihood (en büyük olabilirlik kestirimi) yöntemi kullanılarak çizilen filogenetik ağaçlar bazı farklılıklar göstermesine rağmen monokotil petaloid taksonların dikotiledon geofit taksonlarından ayrıldığı görülmüştür. Ayrıca her iki moleküler marker kullanılarak gerçekleştirilen filogenetik analizlerde Serapias, Anacamptis, Cephalanthera, Hymanthoglossum ve Dactylorhiza cinslerinin Orchis içerisine dahil olduğu görülmüştür. Bu nedenle, bu cinse ait alt türlerin akrabalık ilişkileri bizim sonuçlarımıza göre yeniden değerlendirilmelidir. Dikotil geofitler, Flora of Turkey'deki klasik sistematik düzene göre moleküler filogeni göstermekte ve bu taksonların tür içi ve türler arası ayrımlarının doğruluğunu ortaya koymaktadır.

Anahtar Kelimeler:

Geofit, ITS, trnL-F, moleküler filogeni

Molecular Phylogeny of Some Geophytes Taxa from Turkey; Systematic Approaches

To determine the evolutionary relationships among some members of geophytes collected from their natural habitat in Kulp-Diyarbakır we carried out molecular phylogenetic analysis of one nuclear DNA (nrDNA) region (internal transcribed spacer, ITS) and one chloroplast DNA (cpDNA) region (intergenic spacer region of trnL-F). Despite phylogenetic analysis using maximum likelihood done by two different DNA source show some differences it was identified that monocotyl petaloid taxa differ from dicotyledone geophyte ones. Also both phylogenetic trees reveal that Serapias, Anacamptis, Cephalanthera, Himanthoglossum and Dactylorhiza are nested in Orchis. Therefore the infraspecific relationships of these genera should be re-evaluated according to our molecular phylogenetic study results. Dicotyl geophyts show molecular phylogeny in accordance with the classical systematic order in Flora of Turkey and reveal the accuracy of interspecific and infraspecific distinctions of these taxa.

Keywords:

Geophytes, ITS, trnL-F, Molecular phylogeny,

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