Mikrodalga ile Lotus corniculatusL. var. corniculatusBitkisininUçucu Yağ Analizi ve Antimikrobiyal Aktivitesi

Bu çalışmada,Lotus corniculatusL. var. corniculatusbitkisininuçucu yağı,mikrodalga aparatlı destilasyon ile elde edilmiştir. Elde edilen uçucu yağın kimyasal bileşimi GC/MS, GC/FID cihazı ile aydınlatılmış ve Minimum İnhibisyon Konsantrasyon yöntemi (MIC)kullanılarak antimikrobiyal aktivitesi tayin edilmiştir.Uçucu yağ analizinde,bitkinin % 80.24’lük kısmı tanımlanmış olup, toplam 56 bileşik yapısı aydınlatılmıştır. Ana bileşen sınıfı olarak %30.60 oranında terpen ve terpen benzeri bileşikler, %16.89 oranında hidrokarbonlar, %12.49 aldehitlerden oluştuğu tespit edilmiştir. β-ionon(%4.83), (2E,4E) dekadienal(%4.13), β-pinen (%3.76), karahanoenon (%3.53) bileşikleri uçucu yağ içinde en fazla bulunan bileşikler olarak tespit edilmiştir.Lotus corniculatusbitkisinden ekstrakte edilen uçucu yağın hekzan çözücüsü içinde 51600 μg/mL konsantrasyonunda hazırlanan stok çözeltisinin; Escherichia coli (E. coli) ATCC35218, Yersinia pseudotuberculosis (Y. pseudotuberculosis) ATCC911,Pseudomonas aeruginosa (P. aeruginosa) ATCC43288,Enterococcus faecalis (E. faecalis) ATCC29212, Staphylococcus aureus (S. aureus) ATCC25923, Bacillus cereus (B. cereus) 709 Roma,Mycobacterium smegmatis (M. smegmatis) ATCC607Gram pozitif,Gram negatif bakterilere veCandida albicans (C. albicans)ATCC60193 maya mantarınakarşı antimikrobiyal aktivite testi yapılmışve uçucu yağın sadece maya mantarı üzerinde 645 μg/mL etkin değer gösterdiği tespit edilmiştir.

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