Erzurum Olur Yöresinden Toplanan Berberis vulgaris L. Meyvelerinin Antioksidan Aktivitelerinin Belirlenmesi

Bu çalışma Berberis vulgaris L. meyve ekstraklarında toplam fenolik ve flavonoid madde miktarı ve antioksidan aktivitelerinin belirlenmesi amacıyla yapılmıştır. Folin-Ciocalteu ve alüminyum nitrat yöntemleri kullanılarak toplam fenolik ve flavonoid madde miktarları belirlendi. Hekzan ve metanol ekstraktında fenolik madde miktarı sırasıyla 33,64±1,86 mg GAE g-1 ve 85,98±1,59 mg of GAE g-1, flavonoid madde miktarı ise 94,74±2,59 mg KE g-1 ve 64,74±1,86 mg KE g-1 olarak belirlendi. DPPH• radikal giderme aktivitelerinin, fenolik madde miktarının çok olduğu metanol ekstraklarında, Fe3+-Fe2+ indirgeme kapasitesinin ise flavonoid madde miktarı fazla olan hekzan ekstraklarında daha yüksek olduğu belirlenmiştir. Bulduğumuz sonuçlara göre B. vulgaris’in gıda takviyesi ve doğal antioksidan kaynağı olarak değerlendirilebileceği kanaatine varılmıştır.

Anahtar Kelimeler:

Berberis vulgaris L., antioksidan, DPPH, FRAP

This study was carried out to determine the total amount of phenolic and flavonoid substances and antioxidant activities in Berberis vulgaris L. fruit extracts. Total phenolic and flavonoid substances were determined by using Folin-Ciocalteu and aluminum nitrate methods. The amount of phenolic substance in the hexane and methanol extract was determined as 33.64±1.86 mg GAE g-1 and 85.98±1.59 mg of GAE g-1 respectively, and the amount of flavonoid substance was determined as 94.74±2.59 mg QE g-1 and 64.74±1.86 mg QE g-1 respectively. It was determined that DPPH• radical scavenging activities were higher in methanol extracts with a high amount of phenolic substances, while the reducing capacity of Fe3+-Fe2+ was higher in hexane extracts with a higher amount of flavonoid substances. According to the results we found, it was concluded that B. vulgaris can be considered as a food supplement and a source of natural antioxidants.

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