Bezelyede Enzim Aktiviteleri Üzerine Soğuk Stresinin Etkileri

Araştırma “Tesadüf Parsellerinde iki Faktörlü Faktöriyel Deneme” desenine göre üç tekerrürlü olarak kurulmuştur ve Selçuk Üniversitesi, Ziraat Fakültesi, Tarla Bitkileri Bölümü tam kontrollü araştırma serası ve laboratuvarlarında 2015 yılında yürütülmüştür. Bu araştırma ile soğuk stresine dayanıklı uygun bezelye genotipleri belirlenmiştir. Ayrıca soğuk stresine karşı bitkiler tarafından oluşturulan biyokimyasal, fiziksel özellikler veya fiziksel savunma mekanizmaları ortaya konulmaya çalışılmıştır. Araştırma sonuçlarına göre; soğuk zararı bakımından Melrose, Şahin, Granger, 4053 x Melrose, 4053 x Hadim, Şahin x Hadim ve 3057 x Melrose, peroksidaz içeriği incelendiğinde 3057 x Melrose, 4053 x Melrose, 3029 x Melrose, 3029 x Granger ve 4053 x Hadim, süperoksit dismutaz içeriği incelendiğinde 3053 x Melrose, Şahin x Hadim, 4053 x Melrose, Şahin x Melrose ve 3029 x Melrose ve prolin içeriği incelendiğinde 3031 x Granger, 3055 x Melrose, Ultrillo, 3057 x Hadim ve Şahin x Hadim genotipleri ilk sıralarda yer almıştır. Sonuç olarak, soğuk stresinin bezelye genotiplerinin yapraklarındaki enzim aktiviteleri üzerine etkileri ele alındığında; genotipler içerisinde en dayanıklı olarak 3031 x Granger ve 3055 x Melrose genotipleri ön plana çıkan bu genotipler daha sonra yapılacak olan soğuğa dayanıklılık ıslah çalışmalarında kullanılabilir.

Anahtar Kelimeler:

POX, Prolin, Soğuğa Tepki

Effects of Cold Stress on Enzyme Activities in Peas

This research was conducted in three replications according to the ''Two Factor Factorial Experiment in Random Plots'' design and Selcuk Unıversıty, Faculty of Agriculture, Department of Field Crops, in a fully controlled research greenhouse and laboratories in 2015. Pea genotypes resistant to cold stress were determined, and biochemical, physical properties or physical defense mechanisms created by plants against cold stress were sought. According to the results of the research, if cold harm was examined, Melrose, Sahın, Granger, 4053 x Melrose, 4053 x Hadım, Sahın x Hadım and 3057 x Melrose; if peroxidase content was examined 3057 x Melrose, 4053 x Melrose, 3029 x Melrose, 3029 x Granger and 4053 x Hadım, when superoxıdase content was examined, 3053 x Melrose, Sahın x Hadım, 4053 x Melrose, Sahın x Melrose and 3029 x Melrose and when proline content examined 3031 x Granger, 3055 x Melrose, Ultrıllo, 3057 x Hadım and Sahın x Hadım the genotypes were first. As a result, when the effects of cold stress on the enzyme activities in the leaves of pea genotypes are considered, the most durable genotypes are 3031 x Granger and 3055 x Melrose. These come forward as genotypes that can be used in subsequent studies to breed for cold resistance.

Keywords:

Cold stress, Cold Reaction,

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