Asipimoksun DPPC Model Membranlar Üzerindeki Etkilerinin Kalorimetrik İncelenmesi

Model membranlar, biyolojik membranları taklit eden temel sistemler olarak yaygın şekilde kullanılmaktadır. Bu çalışmada, dipalmitoil fosfatidilkolin (DPPC) ile lipit düşürücü ilaç asipimoks arasındaki fiziksel etkileşim, farklı asipimoks konsantrasyonları ve sıcaklığa bağlı olarak Diferansiyel Tarama Kalorimetri (DSC) tekniği kullanılarak araştırıldı. DPPC içeren model membranların faz geçiş sıcaklığı, faz geçiş entalpisi ve faz geçiş eğrisi yarı yükseklik genişliğindeki değişimler değerlendirildi. DSC sonuçlarına göre saf DPPC model membranlara asipimoks ilavesi ile 41 °C civarındaki ana faz geçiş sıcaklık eğrisi biraz daha yüksek sıcaklıklara kayarken, 35 °C civarındaki geçiş öncesi sıcaklık eğrisi kaybolmadı. Ayrıca, artan asipimoks konsantrasyonları, model membran DPPC'nin DSC termogramlarında hafif bir genişlemeye neden oldu. İlaçların biyomembranlardaki farmakolojik aktivitelerinin anlaşılması onların hücre içi aktivileri açısından çok önemli olduğu için, lipit düşürücü ilaç asipimoks ile lipitler arasındaki etkileşimin araştırılması, asipimoksun moleküler düzeydeki biyolojik etkilerine katkı sağlayabilir.

Anahtar Kelimeler:

Asipimoks, Model Membranlar, DPPC, DSC, İlaç-Membran Etkileşimi, Acipimox, Model Membranes, Drug-Membrane Interaction

A Calorimetric Investigation of the Effects of Acipimox on DPPC Model Membranes

Liposomes or model membranes are widely used basic systems which mimic biological membranes. In this study, the physical interaction between dipalmitoyl phosphatidylcholine (DPPC) and the lipid-lowering drug acipimox was investigated using Differential Scanning Calorimetry (DSC) technique depending on different acipimox concentrations and temperature. The changes in the phase transition temperature, phase transition enthalpy and phase transition curve half-height width of the model membranes were taken into account. According to the DSC results, with the addition of acipimox into pure DPPC model membranes, the main phase transition temperature curve, which is around 41 °C, shifted to slightly higher temperatures, while the pre-transition temperature curve, which is around 35 °C, did not disappear. Moreover, increasing acipimox concentrations caused a slight broadening of the DSC thermograms of the model membrane DPPC. Since it is very important to understand the pharmacological activity of drugs in biomembranes, the investigation of the interaction between lipid-lowering drug acipimox and lipids may contribute to the biological effects of acipimox at the molecular level.

Keywords:

Acipimox, Model Membranes, DPPC, DSC, Drug-Membrane Interaction,

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