Yerli Sığır Irklarında TLR2 -EcoRV, NOD2- BsaHI ve IFNγ- HphI Allelerinin Frekans Dağılımının İncelenmesi

Değişen iklim koşulları ve artan nüfus olası risklere karşı önlem alınması ihtiyacına yol açmıştır. Hayvansal üretimi de içine alan tarımsal alanda çalışan araştırıcılar hastalıklara ve çevresel strese dayanıklı çeşit ve türler bulma ve bunları geliştirme üzerine yoğunlaşmışlardır. Hastalık ve çevresel strese dayanıklılığı etkileyen gen bölgelerinin tespit edilmesi ile bu özellikler bakımından seleksiyon yapılması sağlanabilecektir. Bu nedenle gerçekleştirilen çalışmada beş yerli sığır ırkında genotipik yapı daha önce paratuberculosis ile ilişkili olduğu bildirilmiş TLR2, NOD2 ve IFNγ genlerine ait SNP’ler (sırasıyla EcoRV, BsaHI ve HphI enzimleri kullanılarak) bakımından PCR-RFLP yöntemi ile incelenmiştir. Yerli Güney Sarısı (NSY= 29), Güneydoğu Anadolu Kırmızısı (EAR=35), Boz Irk (AG=36), Güneydoğu Anadolu Kırmızısı (SAR=34), Yerli Kara (NB=35) ırklarından toplam 169 hayvan analiz edilmiştir. Allel ve genotip frekansları PopGene32 programı ile hesaplanmış, populasyonların Hardy-Weinberg dengesinde olup olmadıkları Khi-kare testi ile kontrol edilmiştir. TLR2- EcoRV ve NOD2- BsaHI lokuslarında iki allel (sırasıyla C ve A, C ve T) ve üç genotip belirlenirken, IFNγ- HphI lokusu G alleli bakımından monomorfik bulunmuştur. TLR2- EcoRV lokusunda C alleli NB dışında tüm ırklarda, NOD2- BsaHI lokusunda tüm ırklarda C alleli predominanttı. NOD2- BsaHI lokusu bakımından tüm ırklar HW dengesinde bulunmuş ancak TLR2- EcoRV lokusu bakımından NSY ve SAR ırkları HW’den sapma göstermiştir.

Investigation of TLR2 -EcoRV, NOD2- BsaHI ve IFNγ- HphI allele frequency distribution among Turkish Native Cattle Breeds

Changing climatic conditions and growing population size lead take precautions against potential risks. Researchers who study in the agricultural field, including animal production, focus on finding out variants and breeds resistant to diseases and environmental stress. Detecting gene regions that affect resistance to diseases and environmental stress might increase the performance of the selection in favor of these traits. Due to these reasons in this study, five Turkish native cattle breeds were investigated for SNPs belonging to TLR2, NOD2, and IFNγ (using EcoRV, BsaHI ve HphI enzymes, respectively) reported to be associated with paratuberculosis previously. In total, 169 animals were analyzed from Native Southern Yellow (NSY= 29), East Anatolian Red (EAR=35), Anatolian Grey (AG=36), South Anatolian Red (SAR=34), and Native Black (NB=35). The genotype and allele frequencies were calculated with the PopGene32 program, and Khi square test was performed to determine whether the populations were in Hardy-Weinberg equilibrium (HWE). While at TLR2- EcoRV and NOD2- BsaHI loci, two alleles (A and C, C and T, respectively), IFNγ- HphI locus was found to be monomorphic for the G allele. For TLR2- EcoRV locus, the C allele was found as predominant except NB breed, and the C allele was predominant for all breeds at NOD2- BsaHI locus. However, in all breeds, NOD2- BsaHI locus was at HWE, for TLR2- EcoRV NSY and SAR breeds showed derivation from HWE.

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