Transport Aşamasında Asya Kedi Balıklarının (Pangasius hypophthalmus) Histolojilerinde ve Stres Parametrelerindeki Değişimiler

Asya kedi balıklarının Pangasius hypophthalmus transportasyonu sırasında, stres parametrelerini (glikoz, kortizol, sodyum ve klorür) ve histolojik değişimleri (solungaç, karaciğer, böbrek ve dalak) araştırılmıştır. Bu çalışmada üç farklı deneme yapılmıştır. Bunlar sırasıyla tricaine içermeyen, 40 mg L-1 tricaine ve 60 mg L-1 tricaine içeren gruplardır. Her deneme dört bölümden oluşmuştur. Bunlar kontrol (C), transfer sonrası (T), transport (TP) ve iyileşme (24 h)’dir. Her aşamadan sonra balıklardan kan alınmıştır ve sonra solungaç, karaciğer, böbrek ve dalak örnekleri histolojik inceleme için toplanmıştır. Tricaine içermeyen transport da, glikoz 50-60 mg dL-1’den 70-80 mg dL-1’ye çıkmıştır ve kortizol 30 ng dL-1’den 90 ng dL-1’ye yükselmiştir. Sodyum 127-132 meq L-1’den 138 meq L-1’ye yükselmiştir. Klorür 103 meq L-1’den 107 meq L-1’ye değişmiştir. 40 mg L-1 tricaine ile transportun yapıldığı ikinci denemede, glikoz 50-65 mg dL-1’den 80-90 mg dL-1’ye yükselmiştir ve kortizol 60-70 ng dL-1’den 35-45 ng dL-1’ye düşmüştür. Sodyum 130-140 meq L-1’den 125 meq L-1‘ye ve klorür 110 meq L-1’den 102 meq L-1 ‘ye düşmüştür. 60 mg L-1 tricaine ile transportun yapıldığı ikinci denemede, glikoz 40-55 mg dL-1'’den 25-30 mg dL-1’ye ve kortizol 35-40 ng dL-1’den 25 ng dL-1’ye düşmüştür. Sodyum 126-128 meq L-1’den 119-122 meq L-1’ye ve klorür 110 meq L-1’den 104-106 meq L-1’ye düşmüştür. Deneme sonunda, tricaine kullanımının Asya kedi balıkları transportunun pozitif etkileri sahip olduğunu fark edilmiştir.

Alterations of Stress Parameters and Histology of Asian Catfish (Pangasius hypophthalmus) in Transport

Stress parameter changes (cortisol, glucose, sodium and chloride) and histological alterations (gills, liver, kidney, and spleen) in Asian Catfish (Pangasius hypophthalmus) in transport were investigated. In this study, three separated trials were fulfilled. These were without tricaine, 40 and 60 mg L-1 of tricaine, respectively. Each trial consisted of 4 stages, control (C), transfer (AT), transport (TP) and recovery (24 h). After each stage, blood samples were taken and then, gills, liver, kidney, and spleen samples were collected for histological examination. In the transport without tricaine, the fish glucose increased from 50-60 mg dL-1 to 70-80 mg dL-1 and cortisol increased from 30 ng dL-1 to 90 ng dL-1 in. Sodium was 127-132 meq L-1 in the AT and TP, while 138 meq L-1 in the C and recovery. Cloride changed 103 meq L-1 in the TP, while approximately 107 meq L-1 in C, AT and recovery in the transport without tricaine. In the second trial, the fish glucose increased from 50-65 mg dL-1 to 80-90 mg dL-1 cortisol decreased from 60-70 ng dL-1 to 35-45 ng dL-1 in the transport with 40 mg L-1 of tricaine. Sodium was 130-140 meq L-1 in the C and recovery, while 125 meq L-1 in the AT and TP. Chloride was about 110 meq L-1 in the C, AT and recovery, while 102 meq L-1 in the TP in the transport with 40 mg L-1 of tricaine. In the third trial, the fish glucose was 40-55 mg dL-1 in the C, AT and TP, while 25-30 mg dL-1 in the recovery. Cortisol was 35-40 ng dL-1 in the C and AT, while 25 ng dL-1 in the TP and recovery. Sodium was 126-128 meq L-1 in the C, AT and recovery, while about 119-122 meq L-1 in the TP. Chloride was 110 meq L-1 in the C, AT and recovery, while 104-106 meq L-1 in the TP in the transport with 60 mg L-1 of tricaine. The end of the experiment, it is realized that the usage of the tricaine had positive effects on Asian catfish transport.

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