Zahide Bilgin, Gülay Merve Bayrakal, Emek Dümen, Gözde Ekici

İstanbul’da Tüketilen Salata ve Mezelerde Toxoplasma gondii, Listeria monocytogenes ve Staphylococcus aureus'un Prevalans ve PCR Duyarlılık Karşılaştırması

Bu çalışma, İstanbul'un çeşitli semtlerinde bulunan perakendecilerden toplanan 100 adet salata ve meze örneğinde (50 salata ve 50 meze örneği) Toxoplasma gondii, Listeria monocytogenes ve Staphylococcus aureus insidansını araştırmak amacıyla yapılmıştır. Toxoplasma gondii'nin analizi için sadece PCR prosedürleri kullanılırken, Listeria monocytogenes ve Staphylococcus aureusiçin geleneksel mikrobiyolojik yöntemler ve PCR prosedürleri kullanıldı. Ayrıca tüm pozitif örnekler için PCR spesifitesi ve duyarlılığı hesaplandı. Elde edilen sonuçlara göre 9 (%9) örnekte Listeria monocytogenes, 36 (%36) örnekte Staphylococcus aureus tespit edildi, tüm örneklerde Toxoplasma gondii negatif olduğu gözlendi. PCR duyarlılığı sonuçlarının, Listeria monocytogenes ve Staphylococcus aureus için oldukça spesifik ve doğru olduğu tespit edildi. Salata ve mezelerin, zayıf hijyen koşulları altında işlem görmesinin tüketicinin sağlığını mikrobiyolojik olarak tehdit edebileceği sonucuna varıldı.

Prevalence and PCR Sensitivity Comparison of Toxoplasma gondii, Listeria monocytogenes and Staphylococcus aureus in Salads and Appetizers Consumed in Istanbul

This study was conducted to investigate incidence of Toxoplasma gondii, Listeria monocytogenesand Staphylococcus aureus in 100 samples of salad and appetizers (50 salad and 50 appetizersamples) collected from retailers located various districts of Istanbul. While only PCR procedureswere used for the analysis of Toxoplasma gondii, conventional microbiological methods and PCRprocedures were used for analysis of Listeria monocytogenes and Staphylococcus aureus. AlsoPCR specify and sensitivity for all the positive samples were calculated. According to the results,9 (9%) samples had Listeria monocytogenes, 36 (36%) samples had Staphylococcus aureus, allthe samples were negative for Toxoplasma gondii. It was noted that PCR sensitivity results werequite specific and accurate for both Listeria monocytogenes and Staphylococcus aureus. It wasconcluded that salad and appetizers may seriously threat consumers’ health microbiologically ifthey are processed under poor hygienic conditions.

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