Roumiana Dimova IVANOVA VASSİLEVSKA, Lydia SHTEREVA, Ira STANCHEVA, Maria GENEVA

Drought Stress Responses of Sunflower Germplasm Developed after Wide Hybridization

Response of sunflower germplasms viz. cultivated sunflower H. annuus and two breeding lines H. annuus x T. rotundifolia and H. annuus x V. encelioides developed after wide hybridization were used for identification of drought tolerant sunflower genotypes at the seedling growth stage. Three water stress levels of zero (control), -0.4, and -0.8 MPa were developed using polyethyleneglycol-6000 (PEG-6000). Physiological and biochemical stress determining parameters such as root and shoots length, fresh weight, antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPO), ascorbate peroxidase (APX) and antioxidant metabolite content (total antioxidant capacity, total phenols and total flavonoids content) were compared between seedlings of all three genotypes. Results revealed that sunflower genotypes have similar responses at two osmotic potentials for shoot and root length and fresh weight. The data also showed that drought stresss could induce oxidative stress, as indicated by the increase level of ascorbate peroxidase and guaiacol peroxidase at -04 MPa in H. annuus cv 1114. Although the activity of ascorbate peroxidase and guaiacol peroxidase was differentially influenced by drought, the changes of antioxidant enzyme activities such as catalase, superoxide dismutase, guaiacol peroxidase, and ascorbate peroxidase subjected to drought stress follow a similar pattern in both breeding lines, indicating that similar defense systems might be involved in the oxidative stress injury in sunflowers. Increase in content of phenols and flavonoids were detected for all three genotypes under stress, which showed that these were major antioxidant metabolites in scavenging cellular H2O2.

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Abu-Jdayil B, Mohameed H. 2002. Experimental and modeling studies of flow properties of concentrated yogurt as affected by the storage time, J Food Eng, 52, 359-365.

Ahmad S, Ahmad R, Ashraf MY, Ashraf M, Waraich EA. 2009. Sunflower (Helianthus annuus L.) response to drought stress at germination and seedling growth stages. Pak. J. Bot., 41:647- 654.

Alscher RG, Donahue JL, Cramer CL. 1997. Reactive oxygen species and antioxidants: relationships in green cells. Physiol. Plantarum, 100(2):224-233.

Alscher RG, Erturk N, Heath LS. 2002. Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J. Exp. Bot., 53(372):1331-1341.

Anjum SA, Xie XY, Wang LC, Saleem MF, Man C, Lei W. 2011. Morphological, physiological and biochemical responses of plants to drought stress. Afr. J. Agricul. Res., 6(9):2026-2032.

Ashraf M. 2010. Inducing drought tolerance in plants: Recent advances. Biotechnol. Adv., 28:169-183.

Ashraf M, Foolad M. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environ. Exp. Bot., 59(2):206-216.

Bakalova S, Nikolova A, Nedeva D. 2004. Isoenzyme profiles of peroxidase, catalase and superoxide dismutase as affected by dehydration stress and ABA during germination of wheat seeds. J. Plant Physiol., 30(1):64-77.

Baloglu MG, Kavas M, Aydin G, Öktem HA, Yücel AM. 2012. Antioxidative and physiological responses of two sunflower (Helianthus annuus) cultivars under PEG-mediated drought stress, Turk. J. Bot., 36:707-714.

Beers Jr RF, Sizer IW. 1952. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J. Biol. Chem., 195:133-140.

Benavente-Garcıa O, Castillo J, Lorente J, Ortuno A, Del Río JA. 2000. Antioxidant activity of phenolics extracted from Olea europaea L. leaves. Food Chem., 68:457-462.

Bradford MM. (976. A rapid and sensitive method for the estimation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem., 72:248-254.

Breton C, Gil A, Wargnier J, Serieys H, Berville A. 2012. Transfer of architectural traits from perennial Helianthus mollis Lam. to sunflower (H. annuus L.) and localization of introgression. Euphytica, 186:557-572.

Breton C, Serieys H, Berville A. 2010. Gene transfer from wild Helianthus to sunflower: topicalities and limits. Oléagineux, Corps Gras, Lipides, 17:104-114.

Cakmak I, Strbac D, Marschner H. 1993. Activities of hydrogen peroxide-scavenging enzymes in germinating wheat seeds. J. Exp. Bot., 44(1):127-132.

Claeys H, Inzé D. 2013. The agony of choice: how plants balance growth and survival under water-limiting conditions. Plant Physiol., 162(4):1768-1779.

Csiszár J, Gallé Á, Horváth E, Dancsó P, Gombos M, Váry Z, Tari I. 2012. Different peroxidase activities and expression of abiotic stress-related peroxidases in apical root segments of wheat genotypes with different drought stress tolerance under osmotic stress. Plant Physiol. and Bioch., 52:119-129.

Diaz-Zorita M, Duarte G, Plante E, Fernandez M. 2003. El cultivo de girasol. Asociacion Argentina de Girasol:10. Bs. As, Argentina. doi: 10.1002/9783527665334.fmatter

Faure N, Serieys H, Berville A, Cazaux E. 2002. Occurrence of partial hybrids in wide crosses between sunflower (Helianthus annuus) and perennial species H. mollis and H. orgyalis. Theor. Appl. Genet., 104:652-660.

Foyer CH, Noctor G. 2005. Oxidant and antioxidant signaling in plants: a re-evaluation of the concept of oxidative stress in a physiological context. Plant Cell Environ., 29:1056-107.

Giannopolitis CN, Ries SK. 1977. Superoxide dismutases I. Occurrence in higher plants. Plant physiol., 59(2):309-314.

Griveau Y, Serieys H, Cleomene J, Belhassen E. 1998. Field evaluation of sunflower genetic resources in relation to water supply. Czech. J. Genet .Plant Breed., 34:11-16.

Gunes A, Pilbeam DJ, Inal A, Coban S. 2008. Influence of silicon on sunflower cultivars under drought stress, I: Growth, antioxidant Communications in Soil Science and Plant Analysis, 39(13- 14):1885-1903. and lipid peroxidation.

Guo Z, Ou W, Lu S, Zhong Q. 2006. Differential responses of antioxidative system to chilling and drought in four rice cultivars differing in sensitivity. Plant Physiol. and Biochem., 44(11):828-836.

Hegarty MJ, Barker GL, Brennan AC, Edwards KJ, Abbott RJ, Hiscock SJ. 2008. Changes to gene expression associated with hybrid speciation in plants: further insights from transcriptomic studies in Senecio. Phil. Trans. R. Soc. B., 363:3055-3069 doi:10.1098/rstb.2008.0080.

Hoagland DR, Arnon D. 1950. The water-culture method for growing plants without soil, California Agricultural Experiment Station Circular, 347:1-32.

Imlay JA. 2003. Pathways of oxidative damage. Annu. Rev. Microbiol., 57(1):395-418.

Kahkonen MP, Hopia AI, Vuorela HJ, Rauha JP, Pihlaja K, Kujala TS. 1999. Antioxidant activity of plant extracts containing phenolic compounds. J. Agric. Food Chem., 47:3954-3962.

Kaya MD, Okçu G, Atak M, Çikil Y, Kolsarici Ö. 2006. Seed treatments to overcome salt 17 and drought stress during germination in sunflower (Helianthus annuus L.), Eur. J. Agron., 24:291-295.

La Duke JC. 1982. Revision of Tithonia. Rhodora, p.453-522.

Lin CC, Kao CH. 2000. Effect of NaCl stress on H2O2 metabolism in rice leaves. Plant Growth Regul., 30(2):151-155.

Marcinska I, Czyczylo-Mysza I, Skrzypek E, Filek M, Grzesiak S, Grzesiak MT, Quarrie SA. 2013. Impact of osmotic stress on physiological and biochemical characteristics in drought- susceptible and drought-resistant wheat genotypes. Acta Physiol. Plant., 35(2):451-461.

McKersie BD, Leshem YY. 1994. Stress and Stress Coping in Cultivated Plants. Kluwer Academic Publishes, Dordrecht. pp. 256

Meloni DA, Oliva MA, Martinez CA, Cambraia J. 2003. Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ. Exp. Bot., 49(1):69-76.

Michel B., Kaufmann M. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiol., 51(5):914-916.

Mittler R. 2002. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci., 7:405-410.

Nakano Y, Asada K. 1981. Hydrogen peroxide is scavenged by ascorbate-specific peroxidases in spinach chloroplasts. Plant Cell Physiol., 22:867-880.

Neill S, Desikan R, Hancock J. 2002. Hydrogen peroxide signalling. Curr. Opin. Plant Biol., 5(5):388-395.

Oraki H, Khajani FP, Aghhlikhana M. 2012. Effect of water deficit stress on proline contents, soluble sugars, chlorophyll and grain yield of sunflower (Helianthus annuus L.) hybrids, Afr. J. Biotech., 11:164-168.

Pan Y, Wu LJ, Yu ZL. 2006. Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regul., 49(2-3):157-165.

Petcu E, Pâcureanu JM. 2011. Developing drought and broomrape resistant sunflower 17 germplasms utilizing wild Helianthus species. Helia, 34:1-8

Pfeffer H, Dannel F, Römheld V. 1998. Are there connection between phenol metabolism, ascorbate metabolism and membrane integrity in leaves of boron-deficient sunflower plants? Physiol. Plantarum, 104:479-485.

Pietta PG. 2000. Flavonoids as antioxidants. J. Nat. Products, 63:1035-1042.

Plewa MJ, Smith SR, Wagner ED. 1991. Diethyldithiocarbamate suppresses the plant activation of aromatic amines into mutagens by inhibiting tobacco cell peroxidase. Mutation Res., 247:57-64.

Rauf S. 2008. Breeding sunflower (Helianthus annuus L.) for drought tolerance, Commun. Biom. 16 Crop Sci., 3:29-44.

Reyes-Valdés MH, Gómez-Martínez M, Martínez O, Godínez FH. 2005. Intergeneric hybrid between cultivated sunflower (Helianthus annuus L.) and Tithonia rotundifolia (Mill) Blake. Helia, 28 (43):61-68.

Riesenberg RH, Willis JH. 2007. Plant speciation. Science, 317:910-914.

Rönicke S, Hahn V, Horn R, Grone I, Brahm L, Schnabl H, Friedt W. 2004. Interspecific hybrids of sunflower as a source of Sclerotinia resistance. Plant Breed., 123:152-157.

Scoric D. 2009. Sunflower breeding for resistance of abiotic stresses. Helia, 32:1-16.

Singh D, Malik CP. 2012. Assesment of genetic diversity in Verbesina encelioides populations using randomly amplified polymorphic DNA (RAPD) markers. Intern J Scientific & Technol. Res., 1(4):1-8.

Stancheva I, Geneva M, Yonova P, Markovska YU. 2011. Accumulation of Cd, Pb and Zn in Tribulus terrestris L.grown on industrial polluted soil and plant antioxidant response. Adv. Environ. Biol., 5:300-306.

Tepe B, Sokmen M, Akpulat HA, Sokmen A. 2006. Screening of the Antioxidant Potentials of Six Salvia species from Turkey. Food Chem., 95:200-204.

Turhan H, Baser I. 2004. In vitro and in vivo water stress in sunflower (Helianthus annuus L.). Helia, 27 (40): 227-236.

Tuteja N, Gill SS, Tuteja R. (eds), 2012. Front Matter, in Improving Crop Productivity in Sustainable Agriculture, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim, Germany.

Urbanek H, Kuzniak-Gebarowska E, Herka K. 1991. Elicitation of defense responses in bean leaves by Botrytis cinerea polygalacturonase. Acta Physiol. Plant., 13:43-50.

Vassilevska - Ivanova R, Tcekova Z, Lidansky T. 1999. Intergeneric hybridization of cultivated sunflower Helianthus annuus L. with Tithonia rotundifolia Blake. Compt. Rend. ABS, 52 (1-2): 93 - 96

Vassilevska-Ivanova R, Lidansky T, Tcekova Z. 2000. Observations on the seedling growth in some wild Helianthus genotypes. Compt. Rend. Acad. Bulg. Sci., 53:77-80.

Vassilevska - Ivanova R, Tcekova Z. 2002. An attempt for hybridization Helianthus x Verbesina. Compt. Rend ABS, 55 (9): 99 - 104.

Vassilevska-Ivanova R, Naidenova N. 2005. Intergeneric hybridization between cultivated sunflower and Asteraceae genera: Helianthus annuus x Echinacea purpurea. Compt. Rend. ABS, 58 (6): 729 - 734.

Vassilevska-Ivanova R, Kraptchev B, Stancheva I, Geneva M. 2013. A compact sunflower line produced after cross Helinathus annuus × Verbesina encelioides. Cent. Eur. J. Biol., 8 (5): 492- 498.

Vassilevska-Ivanova R, Kraptchev B, Stancheva I, Geneva M, Iliev I, Georgiev G. 2014a Utilization of related wild species (Echinacea purpurea) for genetic enhancement of cultivated sunflower (Helianthus annuus L.). Turk. J. Agric. For., 38 (1): 15-22.

Vassilevska-Ivanova R, Shtereva L, Kraptchev B, Karceva T. 2014b. Response of sunflower (Helianthus annuus L) genotypes to PEG-mediated water stress. Cent. Eur. J. Biol., 9 (12):1206- 1214.

Vassilevska-Ivanova R, Kraptchev B, Shtereva L. 2015. An intergeneric hybrid line produced after cross Helianthus annuus x Echinacea purpurea. Genet. Resour. Crop Evol. ISSN 0925- 9864 DOI 10.1007/s10722-015-0281-z.

Wagner WL, Herbst DR, Sohmer SH. 1990. Manual of the Flowering Plants of Hawaii, Volume 1. University of Hawaii Press, Bishop Museum Press.

Xiao XW, Xu X, Yang F. 2008. Adaptive Responses to Progressive Drought Stress in Two Populus cathayana Populations. Silva Fennica, 42(5):705-719.

Zhishen J, Mengcheng T, Jianming W. 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chem., 64:555-559.

Zlatanov M, Angelova-Romova M, Antova G, Ivanova E, Damyanova B, Momchilova S, Marecov I. 2008. Phosholipid composition in oils from Bulgarian varieties of sunflower. Plovdiv University ,,Paisii Hilendarski" - Bulg Sci Papers - Chemisry, 36 (5): 105-110.