Bal Arılarının Koşullu Şartlandırılmasında Kullanılan Doygun Atmosfer Spreyi ve Dokunma Yöntemlerinin Dil Çıkarma Refleksi (PER) Üzerine Etkileri
Günümüzde bal arılarında dil çıkartma (PER) ve iğne çıkartma (SER) refleksleri gözlemlenerek koşullu şartlandırma araştırmaları yapılmaktadır. Araştırmalar sonucu narkotik maddeler, patlayıcılar, hastalıklar ve manyetik alanlar gibi olguların tespitinde bal arılarının biyo-sensör olarak şartlandırılarak kullanılabileceği gösterilmiştir. Bal arıları ile yapılan koşullu şartlandırma çalışmalarında doygun atmosfer spreyi ve dokunma yöntemleri dil çıkartma refleksi (PER) tespitinde yoğun olarak kullanılmaktadır. Bal arılarının farklı ırk ve ekotipleri ile yapılan bu çalışmada; doygun atmosfer spreyi ile şartlandırma yöntemi için, şartlandırma kabini içerisindeki işçi arılara aromatik bitki özütü içeren 50 cc atmosferik koku; yaklaşık 3 saniye içerisinde 5 cm mesafeden baş hizasına enjekte edilerek tanıtılmış ve arkasından 1/1 şeker-su (w/w) karışımı şurup ile ödüllendirilmişlerdir. Dokunma ile şartlandırma denemesinde ise yağlı aromatik bitki özütüne daldırılan ahşap kürdan işçi arıların antenlerine dokundurularak arkasından 1/1 şeker-su (w/w) karışımı şurup ile ödüllendirilmişlerdir. 15 dakika ara ile üç kez tekrar edilerek koşullu şartlandırılan bal arılarının şartlanma sonrası hatırlama oranları dil çıkartma refleksi (PER) davranışı gözlemlenerek şartlanma gösterip göstermedikleri tespit edilmiştir. Araştırmada kullanılan bal arısı ırk ve ekotiplerinin her iki yöntemle de şartlandıkları gözlemlenmiştir. Bal arılarının koşullu şartlanma uygulaması sonrası hatırlamada; dokunma uygulamasının doygun atmosfer spreyi uygulamasına göre daha fazla şartlanma ve hatırlanma oranına sahip olduğu tespit edilmiştir.
The Effect of Saturated Atmosphere Spray and Touching Methods Used Conditioning of Honey Bee’s on Proboscis Eject Reflections (PER)
It has been done conditioning experience by observing proboscis eject reflections (PER) and stingejections (SER) on honey bees. Today, many scientists have showed that honey bees can be usedby conditioning for the determination of events such as narcotics, mines, diseases, magnetic fieldsin the experiment (test). Saturated atmosphere spray and touching methods have been mostly usedfor studying of proboscis eject reflections (PER). This study was done with different honey beeraces and ecotypes and in this study, aromatic plant extract containing 50 cc of atmospheric scentwere used for saturated atmosphere sprey's conditioning method. The worker bees in theconditioning chamber have been presented by injecting to head level from the 5 centimeterdistance approximately in 3 seconds and then they have been rewarded with a syrup mixture ofcontaining a split a (1/1 w/w) sugar and water. In the touching conditioning method, by thetouching of worker – bee, antennas with a wooden toothpick dips to oil aromatic plant extract. Asingle toothpick has been used for every worker bee that has been recorded whether it has showedor not proboscis eject reflections (PER) by repeating tree times of these processes with a quarterbreaks. In the experiment, it has been observed that all the races and ecotypes of honey bees usedin the experiment have showed conditioning in both two methods in the statistical analysis of therecorded data. An important difference has been observed in the conditioning and rememberingamong the application methods. At the end of the experiment, it has been observed that touchingapplication has more conditioning and remembering rates than saturated spray application inconditioning and remembering. In statistical meaning, these two applications have occurred in twodifferent groups.
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- Abramson CI. 1990. Invertebrate Learning A Laboratory Manual
and Source Book. Washington DC: American Psychological
Association.
- Abramson CI, Boyd BJ. 2001. An Automated Apparatus For
Conditioning Proboscis Extension in Honeybees (Apis
Mellifera ). Journal Of Entomological Science, 36: 78-92.
- Abramson CI, Mixson TA, Çakmak I, Place AJ, Wells H. 2008.
Pavlovian Conditioning Of The Proboscis Extension Reflex
in Harnessed Foragers Using Paired Vs. Unpaired And
Discrimination Learning Paradigms: Tests For Differences
Among Honeybee Subspecies in Turkey. Apidologie; 39:
428-435
- Abramson CI, Wells H, Bozic J. 2007. A Social İnsect Model For
The Study Of Ethanol İnduced Behavior: The Honey Bee. In:
Yoshida R (Ed) Trends İn Alcohol Abuse And Alcoholism
Research. Nova Science, Hauppauge,; Pp 197–218
- Anonymous. 2014 FAO statistic http://www.fao.org/
faostat/en/#data/QA[Access:21. July.2017]
- Avarguès-Weber A, Deisig N, Giurfa M. 2011. Visual Cognition
İn Social İnsects. Annu Rev Entomol; 56: 423–443
- Bernadou A, Démares F, Couret-Fauvel T, Sandoz JC, Gauthier
M. 2009. Effect Of Fipronil On Side-Specific Antennal
Tactile Learning İn The Honeybee. J Insect Physiol; 55:
1099–1106
- Chen L, Zhang S, Srinivasan MV. 2003. Global Perception İn
Small Brains: Topological Pattern Recognition İn Honey
Bees. Proc Nat Acad Sci U S A. 100: 6884–6889
- Dacher M, Gauthier M. 2008. Involvement Of NO-Synthase And
Nicotinic Receptors İn Learning İn The Honey Bee. Elsevier.
2008; 200-2007.
- Dyer AG, Spaethe J, Prack S. 2008. Comparative Psychophysics
Of Bumblebee And Honeybee Colour Discrimination And
Object Detection. J Comp Physiol A 194: 617–627
- Erber J, Kierzek S, Sander E, Grandy K. 1998. Tactile Learning
İn The Honeybee. J Comp Physiol A; 183: 737–744
- Faber T, Joerges J, Menzel R. 1999. Associative Learning
Modifies Neural Representations Of Odors İn The İnsect
Brain. Nature Neurosci; 2: 74–78
- Frost EH, Shutler D, Hillier NK. 2013. Effects Of Fluvalinate On
Honey Bee Learning, Memory, Responsiveness To Sucrose,
And Survival. The Journal Of Experimental Biology, 216:
2931-2938.
- Fröhlich B, Riederer M, Tautz J. 2000. Comb-Wax
Discrimination By Honeybees Tested With The Proboscis
Extension Reflex. J Exp Biol; 203:1581–1587
- Getz WM, Smith KB. 1987. Olfactory Sensitivity And
Discrimination Of Mixtures İn The Honeybee Apis Mellifera.
J Comp Physiol A; 160: 239–245
- Giurfa M. 2003. Cognitive Neuroethology: Dissecting NonElemental Learning İn A Honeybee Brain. Curr Opin
Neurobiol; 13: 726–735
- Giurfa M. 2007. Behavioral And Neural Analysis Of Associative
Learning İn The Honeybee: A Taste From The Magic Well. J
Comp Physiol A; 193: 801–824
- Giurfa M, Zhang S, Jenett A, Menzel R, Srinivasan MV. 2001.
The Concepts Of ‘Sameness’ And ‘Difference’ İn An İnsect.
Nature; 410: 930–933
- Giurfa M. 2007. Behavioural And Neural Analysis Of
Associative Learning İn The Honey Bee: A Taste From The
Magic Well. Journal Of Comparative Physiology; A193: 801-
824. Http://Dx.Doi.Org/10.1007/S00359-007-0235-9
[Access:17.july.2017]
- Giurfa M, Malun D. 2004. Associative Mechanosensory
Conditioning Of The Proboscis Extension Reflex İn
Honeybees. Learning Memory; 294-302.
- Giurfa M, Hammer M, Stach S, Stollhoff N, Müller-Deising N,
Mizyrycki C. 1999. Pattern Learning By Honey Bees:
Conditioning Procedure And Recognition Strategy. Animal
Behaviour; 57: 315-324.
- Laska M, Galizia CG, Giurfa M, Menzel R. 1999. Olfactory
Discrimination Ability And Odor Structure–Activity
Relationships İn Honeybees. Chem Senses; 24: 429–438
- Menzel R, Shmida A. 1993. The Ecology Of Flower Colours And
The Natural Colour Vision Of İnsect Pollinators: The Israeli
Flora As A Study Case. Biol Rev; 68: 81–120
- Menzel R, Müller U. 1996. Learning And Memory İn Honeybees:
From Behavior To Neural Substrates. Annual Review Of
Neuroscience,; 19: 379-404. Doi:10.1146/Annurev.
Ne.19.030196.002115
- Niggebrügge C, Leboulle G, Menzel R, Komischke B, Hempel
De Ibarra N. 2009. Fast Learning But Coarse Discrimination
Of Colours İn Restrained Honeybees. J Exp Biol; 212: 1344–
1350
- Scheiner R, Kuritz-Kaiser A, Menzel R. 2005. Sensory
Responsiveness And The Effects Of Equal Subjective
Rewards On Tactile Learning And Memory Of Honeybees.
Learning Memory; 626-635.
- Stevanus Rio T, Martin G. 2013. Rules And Mechanisms Of
Punishment Learning İn Honey Bees: The Aversive
Conditioning Of The Sting Extension Response. The Journal
Of Experimental Biology; 216, 2985-2997 Published By The
Company Of Biologists Ltd Doi:10.1242/Jeb.086629
- Zhang SW, Lehrer M, Srinivasan MV. 1999. Honeybee Memory:
Navigation By Associative Grouping And Recall Of Visual
Stimuli. Neurobiol Learn Mem; 72:180–201