Allium Testi ile Floksinin Genotoksik Etkisinin Değerlendirilmesi

Floksin, gıda boyası olarak kullanılmaktadır. Bu çalışmada, kırmızı renkli olan floksinin Allium cepa L. kök ucundaki genotoksik etkisi araştırılmıştır. A. cepa L. meristematik kök ucu hücreleri, on farklı dozda floksin ile muamele edilmiştir. Bu şekilde EC50 değeri belirlenmiştir. Daha sonra kök uçlarına EC50/2, EC50 ve EC50×2 dozlarında floksin uygulanmıştır. Floksin ile muamele süresi 24, 48 ve 72 saat olarak belirlenmiştir. Araştırmanın sonucunda, A. cepa kök ucu mitotik döngüsündeki hücrelerde floksinin kromozomal aberasyonlara neden olduğu ortaya konmuştur. Mitotik hücrelerde gözlenen kromozomal aberasyonlar arasında metafazda ekvatoral plak kayması, gecikmeli kromozom, bozulmuş iğ iplikleri, kromozom yapışkanlığı, C-mitoz, kutup kayması bulunmaktadır. Kök uçlarına uygulanan floksin dozu artışına bağlı olarak, % kromozomal aberasyon indeksinin arttığı belirlenmiştir. En yüksek % kromozomal aberasyon indeksinin ise, EC50×2 dozunun 72 saat süresince uygulanması ile meydana geldiği ortaya konmuştur. Araştırmanın sonucuna göre, floksinin A. cepa kök ucunda genotoksik etkisi olduğu saptanmıştır

Evaluation of Genotoxic Effect of Phloxine by Allium Test

Phloxine is used as a food dye. In this study, genotoxicity of phloxine at the root tip of Allium cepa L. was investigated. A. cepa L. meristematic root tip cells were treated with ten different doses of phloxine. In this way, the EC50 value was determined. Then, phloxine was applied to root tips at EC50/2, EC50 and EC50×2 doses. Treatment time was determined as 24, 48 and 72 hours. As a result, it was revealed that phloxine caused chromosomal aberrations in cells in mitotic cycle at the root tip of A. cepa. There are equatorial plate shifting in metaphase, laggard chromosome, disturbed spindle, chromosome stickiness, C-mitosis, polar shifting among the observed chromosomal aberrations. It was stated that the % chromosomal aberration index (CAI) increased depending on concentration increase. It has been demonstrated that the highest % chromosomal aberration index occurred at the EC50×2 dose for 72 hours. According to the research, it was revealed that phloxine has a genotoxic effect on the root cells of A. cepa. For this reason, it can be emphasized that care should be taken in its use in foods

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