Özlem AYDEMİR, Hüseyin Agah TERZİ, Mehmet KÖROĞLU, Gupse TURAN, Mustafa ALTINDİŞ, Engin KARAKEÇE

Servikal örneklerde HPV-DNA Pozitiflik Oranı ve Sitopatolojik Sonuçların Değerlendirilmesi

Amaç: Servikal kanser tüm dünyada kadınlar arasında en yaygın görülen ikinci kanser türüdür. Human papilloma virus HPV servikal kanser ile ilişkisi gösterilmiş majör etiyolojik ajandır. HPV’nin bugüne kadar 200’den fazla türü belirlenmiş ve bunlardan 40 tanesinin genital sistemde enfeksiyon yaptığı bilinmektedir. Bu çalışmanın amacı; HPV DNA araştırılması için laboratuvarımıza gönderilen servikal örneklerde HPV DNA varlığını ve HPV genotiplerini belirlemek, aynı zamanda HPV pozitif hastalarda gelişen sitopatolojik değişiklikleri incelemektir.Yöntem: 01 Ocak 2015 - 30 Ocak 2018 tarihleri arasında Kadın Hastalıkları ve Doğum polikliniğine başvuran 20-66 yaş arası hastalardan alınan 1068 servikal sürüntü örneği çalışmaya dahil edildi. Alınan örneklerde DNA izolasyonu için QIAamp®DNA Mini Kit Qiagen, Almanya , PCR ve pyrosequencing yöntemi ile HPV tiplendirme aşamasında HPVsign® Q24 complate Diatech Pharmocogenetics, İtalya kiti kullanıldı.Bulgular: 1068 örneğin 226 %21,1 tanesinde HPV DNA pozitif olarak saptandı. HPV DNA pozitif bulunan örneklerin 141’inde %62.3 yüksek riskli tipler tek başına saptandı. HPV DNA pozitif bulunan örneklerin içinde 73 %32,3 hastada tek başına HPV tip 16 saptanırken 37 types were found in 37 16.3% patients and other types were found in 116 patients. HPV DNA positivity was highest in the 20-30 age group 26.3% . Cytology positivity was found in 20 8.8% of the patients with HPV DNA positivity. Of these patients, 13 had LSIL 65% , 4 20% had HGSIL, 1 5% had ASCUS, 2 10% had HGSIL / seviks CA.Conclusion: This is the first comprehensive study conducted in our region. The rate of HPV DNA positivity detected in cervical swab specimens and the frequency of HPV type 16 were found to be somewhat higher than the rates found in other studies in our country. More extensive work is needed to determine the type of HPV DNA and HPV type prevalence and the types of HPV that should be used in our country

Anahtar Kelimeler:

Human papilloma virus, servikal kanser, prevalans

Evaluation of HPV-DNA Positivity Rate and Cytopathologic Results in Cervical Specimens

Objective: Cervical cancer is the second most common cancer type among women all over the world. Human papilloma virus HPV is the major etiologic agent associated with cervical cancer. More than 200 species of HPV have been identified so far and 40 of them are known to infect the genital system. The aim of this study was to determine the presence of HPV DNA and HPV genotypes in the cervical samples sent to our laboratory for HPV DNA investigation and to investigate the cytopathological changes in HPV positive patients.Methods: A sample of 1068 cervical swabs taken from patients between the ages of 20 and 66 applied to the Obstetrics and Gynecology Clinic between 01 January 2015 and 30 January 2018 were included in the study. HPVsign® Q24 complate kit Qiagen, Germany was used for HPV typing with QIAamp® DNA Mini Kit PCR Diatech Pharmocogenetics, Italy and pyrosequencing for DNA isolation.Results: HPV DNA was detected as positive in 226 21.1% samples of 1068 samples. In 141 62.3% of the HPV DNA positive specimens, high-risk types were detected alone. Among HPV DNA positive samples, HPV type 16 was found in 73 32.3% patients alone, multiple ailesine ait, zarfsız, 55 nm çapında, ikozahedral Human Papillomavirus HPV Papillomaviridae types were found in 37 16.3% patients and other types were found in 116 patients. HPV DNA positivity was highest in the 20-30 age group 26.3% . Cytology positivity was found in 20 8.8% of the patients with HPV DNA positivity. Of these patients, 13 had LSIL 65% , 4 20% had HGSIL, 1 5% had ASCUS, 2 10% had HGSIL / seviks CA.Conclusion: This is the first comprehensive study conducted in our region. The rate of HPV DNA positivity detected in cervical swab specimens and the frequency of HPV type 16 were found to be somewhat higher than the rates found in other studies in our country. More extensive work is needed to determine the type of HPV DNA and HPV type prevalence and the types of HPV that should be used in our country

Keywords:

Human papilloma virus, cervical canser, prevalence,

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