Fatma ÖZTÜRK KÜP, Burçin KOÇAK, Ali Tuğrul AKIN, İsrafil DOĞANYİĞİT, Aslı OKAN, Emin KAYMAK, Züleyha DOĞANYİĞİT

Lipopolisakkarit’in neden olduğu bağırsak toksisitesine karşı biyosentetik gümüş nanopartiküllerin etkisi

Amaç: Nanopartiküller, sahip oldukları üstün fiziksel özellikleri nedeniyle biyoteknoloji, farmakoloji, tıp, sensörler, bilişim ve iletişim, elektronik, savunma, tekstil, makine ve inşaat sanayileri de dahil olmak üzere bir çok alanlarda kullanılmaya başlanmıştır. Biyolojik sentez yöntemleri toksik kimyasallar kullanılmadığından dolayı farmasötik ve diğer biyomedikal uygulamalar için çevre dostu özellikleri nedeniyle son yıllarda yaygın olarak kullanılmaktadır. Mikroorganizmalar ve bitki ekstraktları ile nanopartiküllerin biyolojik sentezi ile ilgili çalışmalar günümüzde hızla artmıştır. Bitki orijinli polifenolik bileşikler son zamanlarda çalışmalarda yoğun şekilde kullanılmaktadır. Bu çalışmada lipopolisakkarit LPS ile oluşturulan bağırsak hasarı ve apoptozis üzerine üzüm çekirdeği ekstresi ile hazırlanan biyosentetik gümüş nanopartiküllerin etkisinin araştırılması amaçlandı. Yöntem: Çalışmada 80 adet Wistar albino türü yetişkin erkek sıçanlar; kontrol grubu, LPS uygulama grubu, üzüm çekirdeği ekstresi grubu, gümüş nanopartikül AgNP grubu, Ag iyonu grubu ve LPS+ Ag iyonu grubu, LPS+ AgNP grubu, LPS+ üzüm çekirdeği özütü grupları olmak üzere rastgele sekiz eşit gruba ayrıldı. Hayvanlardan alınan bağırsak dokularında histopatolojik değerlendirme ve TUNEL yöntemi ile apoptotik hücre sayıları değerlendirildi. Bulgular: Çalışmanın sonucunda LPS uygulamasının bağırsaklarda nekrotik villuslarda lümene dökülme, villuslarda dejeneratif değişiklikler ve kanamaya neden olduğu, Ag iyonu, üzüm çekirdeği özütü ve AgNP’lerin LPS ile birlikte uygulanması ile bu değişikliklerin hafiflediği görülmüştür. Ayrıca LPS apoptotik hücre sayısını da diğer gruplara göre anlamlı derecede artırmış ve Ag iyonu, üzüm çekirdeği ekstresi ve AgNP’lerin LPS ile birlikte uygulanması ise apoptotik hücre sayısı üzerine istatistiksel olarak olumlu etki göstermiştir. Sonuç: Sonuç olarak biyosentetik AgNP’lerin LPS’nin neden olduğu toksisiteyi azaltacak potansiyele sahip olduğunu söyleyebiliriz. Bu çalışma bundan sonraki çalışmalara ışık tutacak niteliktedir.

Anahtar Kelimeler:

LPS, biyosentetik gümüş nanopartikül, üzüm çekirdeği ekstresi, bağırsak, TUNEL

The effect of biosynthetic silver nanoparticles against intestinal toxicity caused by lipopolysaccharide

Objective: Nanoparticles have been used in many fields including biotechnology, pharmacology, medicine, sensors, informatics and communications, electronics, defense, textile, machinery and construction industries due to their superior physical properties. Biological synthesis methods have been widely used in recent years due to their environmentally friendly properties for pharmaceutical and other biomedical applications since toxic chemicals are not used. Studies on the biological synthesis of microorganisms and plant extracts and nanoparticles have increased rapidly today. Plant origin polyphenolic compounds have been used extensively in recent studies. In this study, it was aimed to investigate the effect of biosynthetic silver nanoparticles prepared with grape seed extract on intestinal damage and apoptosis caused by lipopolysaccharide LPS . Methods: In the study, 80 rats of Wistar albino were male rats; control group, LPS group, grape seed extract group, silver nanoparticle AgNP group, Ag ion group and LPS + Ag ion group, LPS + AgNP group, LPS + grape seed extract groups were randomly divided into eight groups. Apoptotic cell counts were evaluated by histopathological evaluation and TUNEL method in intestinal tissues from animals. Results: As a result of the study, it has been observed that LPS application causes lumen pouring in necrotic villi in the intestines, degenerative changes and bleeding in villi, and these changes are alleviated with the application of Ag ion, grape seed extract and AgNPs together with LPS. In addition, LPS significantly increased the number of apoptotic cells compared to other groups, and the application of Ag ions, grape seed extract and AgNPs together with LPS showed a statistically positive effect. Conclusion: As a result, It can be said that biosynthetic AgNPs have the potential to reduce the toxicity caused by LPS. This study will shed light on future studies.

Keywords:

LPS, biosynthetic silver nanoparticle, grape seed extract, intestine, TUNEL,

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