Daminozit'in tavşan karaciğer glutatyon-s-transferaz aktivitesine etkisinin araştırılması

Çalışmada tavşan karaciğerinden izole edilen Glutatyon- S - Transferaz'ın etkili bir pestisit olan daminozit ile etkileşimi incelenmiştir. Glutatyon -S-Transferaz enzimi in vitro şartlarda değişik dozlarda daminozit ile muamele edilerek, reaksiyonları gözlenmiştir. Bulgular 1-klora 2,4 dinitro benzen (CDNB) substrat olarak kullanıldığında elde edilen aktivite ile karşılaştırılmıştır. Regresyon ve korelasyon analizi yapılmış ve sonuçlar spesifik aktivite olarak verilmiştir.

An investigation on effect of daminozide to the glutation-s-transferase activity in rabbit liver

We analyzed the interaction between glutathion-S-transferase, which is isolated from the liver of rabbits, and daminozide which is an effective pesticide. Glutathion-S-transferase enzymes were treated with daminozide in vitro conditions at various doses, and the reactions were recorded. The observed activities were compared with the activity of 1-chloro 2, 4 dinitrobenzen substrate and a regression analysis was performed to investigate the correlation. The result were expressed as specific activities.

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  • 1.Nickell LG. Plant growth regulating Chemicals Vol 2. CRC Pres. Inc Boca Ratr ISBN 08493-5009 (6) Florida USA1983
  • 2.Kvien C.S, De Palma R A, Raczynski A R; Effect of soil and foliar daminozide application on residüe levels in peanut J Agric Food Chem 1989; 37: 200-3
  • 3.Mol HG, Van Dam RC, Vreeken RJ, Steijger OM. Determination of daminozide in apple leaves by liquid chromatography-mass spectrometry. J Chromatogr A 1999; 12: 833(1): 53-60.
  • 4.Mott L. Alar again: science, the media, and the public's right to know. Int J Occup Environ Health 2000; 6 (1):68-70.
  • 5.Mott L. Alar the aftermath. Science 1992; 7: 25 (5045) 665.
  • 6.Sagelsdorff P, Lutz W.K, Schlatter; DNA methylation in rat liver by daminozide, 1.1 dimethylhydrazine and dimethylnitrosamine. Fundem Appl Toxic 1988; 11: 723-730
  • 7.Murray KR, Mayes P. A, Granner D. K, Rodwell WV. Harper'ın Biyokimyası I.Böl. 1993; 60: 811-7
  • 8.Zubay G. Biochemistry. 3rd Ed. Addisson Wesley CoUSA 1983; 543.
  • 9.Boyer TD, Vessey D.A, Holcomb C, Soly N; Studies of the relatIonship between the catalitic activitiy and binding of nonsubstrate ligands by GST. Biochem J 1984; 217: 179-85.
  • 10.Habig W, Jacoby W. Methods in Enzymology 1981; 77: 213-31 .
  • 11.Habig W, Pabst M, Jacoby W. GlutatIon-S-transferases. J Biol Chem 1974; 249 (25): 7130-9.
  • 12.Kaleti G, Leder WH. Protein-Lowry handbook of micromethods for the biological sciences. New York,Van Nostrand Reinhold Co. 1974;87-88.
  • 13.Sümbüloğlu K, Sümbüloğlu V. Biyoistatistik. 4. Baskı Özdemir Yayın. Ankara.1993
  • 14.Papdopoulou-Mourkidou E. Postharvest- applied agrochemicals and their residues in fresh fruits and vegetables. J Assoc Anal Chem 1991; 74 (5): 745-6
  • 15.Korkmaz M, Çolak A, Sezgin I. Fare kemik iliği hücrelerinde in vivo olarak mikronukleus testi ile daminozitin etkisinin incelenmesi. Tr J Biology 1994
  • 16.Cabral R, Hakoi K, Hoshiya T, Hasegewa R, Ito N. Lack of carcinogenicity of daminozide, alone or in combination with its contaminant 1,1 dimethylhydrazine,in medium-term bioassay.Terat Carcinog Mutagen 1995-96 ;15 (6): 307-12.
  • 17.Hasegewa R, Cabral R,Hoshiya T, Hakoi K. Carcinogenic potential of some pesticides in a medium- term multi organ bioassay in rats. Int J Cancer. 1993; 28; 54 (3): 489-93
  • 18.Göze İ, Yelkovan I, Çınar Z; Daminozitin civcivlerdeki enzimatik etkileri ve histopatolojisi. Tr J Biology 1995; 19:217-22
  • 19.Koshkarian AO, Kocharian ES, Aloian GA, Avakian AKh. Effect of plant growth regulators, hydrazine derivates,on the state of the microzomal systems of the liver. Biull Eksp Biol Med. 1988 ; 106 (7): 40-2.
  • 20.Vessey DA, Boyer TD. Differential activation and inhibition of diferent forms of rat liver GST by the herbicides 2-4 D and 2-5 D. Toxic Appl Pharm 1984; 73: 492-9 .
  • 21.Cabral R, Hoshiya T, Hakoi K, Hasegawa R,Fukushima S, Ito N. A rapid in vivo bioassay for the carcinogenicity of pesticides. Tumor 1991; 77 (3): 185-8.
  • 22.Siliğ Y, Çelik VK, Atalay A. Daminozit uygulanmasından sonra elde edilen civcivlerde karaciğer sitoplazmik GST ve mikrozomal nitrozo dimetilamin demetilaz aktivitesindeki değişiklikler. Tr J Biology 2000; 24; 119-26.