Çeşitli dondurma yöntemlerinin HEp-2 ve tavşan böbrek hücrelerinin kriyoprezervasyonda hücre canlılığına etkisi
Bu çalışmada bovin serum albumininin, primer tavşan böbrek hücre kültürü ile HEp-2 devamlı hücre kültürlerinin kriyoprezervasyonunda hücre canlılığı üzerindeki rolü araştırıldı. Öncelikle hücrelerin yavaş, orta hızlı ve hızlı dondurma metodlarıyla kriyoprezervasyonu yapıldı. Kriyoprotektan madde olarak dimetil sülfoksit, gliserol ve Rowe karışımı kullanıldı. Hücreler %5 ve %10'luk kriyoprotektan madde ve 0, 5, 10, 20 ve 30g/mL bovin serum albumin içeren dondurma solüsyonları ile donduruldu. Deneylerde en yüksek hücre canlılığı yavaş dondurma metodunda elde edilirken, bu metotda dondurma solüsyonuna katılan bovin serum albuminin konsantrasyonuyla doğru orantılı olarak hem primer tavşan böbrek hem de HEp-2 devamlı hücrelerinin canlılığı üzerinde istatiksel olarak anlamlı bir artış meydana getirdiği tespit edildi (p0.05).
The effect of the various freezing methods on the cell viability of cryopreserved HEp-2 and rabbit kidney cells
In this study, the role of bovine serum albumin on the viability of cryo-preserved primary rabbit kidney cell- and HEp-2 continuous cell cultures was studied. Firstly, the cryopreservations of cells were done via slow, intermediate and rapid freezing methods. Dimethyl sulfoxide, glycerol and Rowe mix were used as cryoprotectant agents. The cells were frozen with the solutions containing 5% and 10% cryoprotectant agents and 0, 5, 10, 20, 30g/mL bovine serum albumin, separately. While the highest cell viability was obtained with slow freezing method, in this method, it has been determined that the concentration of bovine serum albumin in the freezing solution proportionally increased the cell viability both on primary rabbit kidney cells and HEp-2 continuous cells (p0.05).
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- ISSN: 0377-9777
- Başlangıç: 1938
- Yayıncı: Türkiye Halk Sağlığı Kurumu
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