İpek Işık GÖNÜL, Gökçen EMMEZ, Ulaş KUMBASAR, Zuhal YILDIRIM, Harun DEMİRCİ, Memduh KAYMAZ, Hakan EMMEZ

Hayvan modelinde alfa-lipoik asit önkoşullaması ile spinal kord iskemi-reperfüzyon hasarından korunma

Amaç: Bu çalışmada deneysel bir iskemi-reperfüzyon yaralanması modelinde alfa-lipoik asit ile önkoşullamanın nöronal hasar üzerinde koruyucu etkisi olup olmadığı araştırıldı. Çalışma planı: On sekiz erişkin erkek Yeni Zelanda tavşanı (2.4-3.5 kg) eşit olarak sham, kontrol ve tedavi gruplarına ayrıldı. Kontrol ve tedavi gruplarında abdominal aort renal artere proksimal olarak 1 cm aşağıdan ve bifürkasyona distal olarak 1 cm yukarıdan anevrizma klipsleri kullanılarak 30 dakika tıkandı. Tedavi grubuna aortik kros-klemplemeden 20 dakika önce intraperitoneal 100 mg/kg lipoik asit uygulandı. Hayvanlar ameliyattan 48 saat sonra sakrifiye edildi ve L2 ve L5 arasındaki spinal kord segmentleri biyokimyasal ve histopatolojik analiz için çıkartıldı. Spinal kordda glutatyon, malondialdehit, total nitrat/nitrit, ileri oksidasyon protein ürünleri, katalaz, süperoksit dismutaz ve glutatyon peroksidaz seviyeleri incelendi. Bulgular: Alfa-lipoik asit ile önkoşullama oksidatif stresin ölçülen tüm parametrelerinde anlamlı olarak olumlu etkiler gösterdi. Dokuların histopatolojik değerlendirmesinde kontrol grubuna kıyasla tedavi grubunda anlamlı olarak azalmış nöronal dejenerasyon, aksonal hasar, mikroglial ve astrositik infiltrasyon görüldü. Sonuç: Bu çalışmanın sonuçları aortik kros-klemplemeden önce alfa-lipoik asit uygulamasının tavşanlarda spinal kord yaralanmasında anlamlı nöro-koruyucu etkisi olduğuna işaret etmektedir.

Protection from spinal cord ischemia-reperfusion damage with alpha-lipoic acid preconditioning in an animal model

Background: This study aims to investigate whether preconditioning with alpha-lipoic acid has any protective effect in neuronal damage in an experimental spinal cord ischemia-reperfusion injury model.Methods: Eighteen adult male New Zealand rabbits (2.4-3.5 kg) were equally divided into sham, control and treatment groups. The abdominal aorta was occluded for 30 min proximally 1 cm below the renal artery and distally 1 cm above the bifurcation using aneurysm clips in control and treatment groups. Treatment group received intraperitoneal 100 mg/kg lipoic acid 20 min before aortic cross-clamping. The animals were sacrificed 48 hours after the operation and spinal cord segments between L2 and L5 were removed for biochemical and histopathological analysis. Levels of glutathione, malondialdehyde, total nitrate/nitrite, advanced oxidation protein products, catalase, superoxide dismutase, and glutathione peroxidase were examined in spinal cord.Results: Preconditioning with alpha-lipoic acid demonstrated significantly favorable effects in all measured parameters of oxidative stress. Histopathological evaluation of the tissues also demonstrated significantly decreased neuronal degeneration, axonal damage, and microglial and astrocytic infiltration in the treatment group compared to the control group. Conclusion: The results of this study indicate that alpha-lipoic acid administration before aortic cross-clamping has significant neuroprotective effect on spinal cord injury in rabbits.

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