Pınar ERECEVİT, Güzin PIHTILI, Sevda KIRBAĞ

İnsanlarda Ciddi Hastalıklara Neden Olan Bakteri, Maya ve Dermatofit Mantarları Üzerinde Poly (DMAA-co-MMA) 'nin İnhibe Edici Etkisi

Bu çalışmada insanlarda ciddi hastalıklara neden olan bakteri, maya ve dermatofit mantarları gibi mikroorganizmalar üzerindeki poli dimetilakrilamid-ko-metilmetakrilat’ın poli P (DMAA-co-MMA) diklorometan içeren çözeltilerinin inhibitör etkilerini araştırıldı. Disk difüzyon yöntemi ile incelenen bu çözelti, Bacillus megaterium ve Klebsiella pneumoniae dışındaki tüm bakterilerin (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) ve dermatofit mantarlarının çoğalmasını engelleyerek antimikrobiyal özelliğe sahiptir. Antimikrobiyal duyarlılık verilerinde, diklorometan içinde çözünen poli P (DMAA-ko-MMA) maya ve dermatofit mantarlarının büyümesine karşı inhibe edici bir etkiye sahiptir (Candida spp. üzerinde 11.3mm / inhibisyon alanı - 12.3 mm / inhibisyon alanı) ve Epidermophyton sp. üzerinde 11.3 mm / inhibisyon alanı - Trichophyton sp. üzerinde 11.3 mm / inhibisyon alanı) (P <0.001). Disk difüzyon yöntemini güçlendiren MIC (Minimal inhibisyon konsantrasyonu) sınır değerleri bakteriler, mayalar, dermatofitlerin büyümesini engelleyen en küçük değer olarak 50-100 μL'dir (10 mL de 4500- 9000 μg). Antimikrobiyal bileşik, gelecek bu alandaki çalışmaları aydınlatmak için büyük avantaj sağlayabilir. Çalışmada kullanılan polimer, iltihaplanma ve mantar enfeksiyonlarına neden olan mikroorganizmalar ile savaşan antimikrobiyal polimerlere umut verici yeni bir katkı sağlayacaktır.

Anahtar Kelimeler:

Yeni antimikrobiyal polimerik bileşik, İnhibitör etki, Poli (DMAA-co-MMA)

The Inhibitory Effect of Poly (DMAA-co-MMA) on Bacteria, Yeast and Dermatophyte Fungi Which Cause Serious Illnesses in People

In this study, it was researched the inhibitor effects of solutions including dichloromethane of poly dimethylacrylamide-co-methylmethacrylate P(DMAA-co-MMA) on microorganisms such as bacteria, yeast and dermatophyte fungi which cause serious illnesses in people. This solution, which was examined by the disc diffusion method, has antimicrobial feature upon preventing the proliferation of all bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) and dermatophyte fungi except Bacillus megaterium and Klebsiella pneumoniae. In antimicrobial susceptibility data; poly P(DMAA-co-MMA) dissolved in dichloromethane has an inhibitory effect against the growth of yeast and dermatophyte fungi (on Candida spp. with 11.3 mm / inhibition area - 12.3 mm / inhibition area and 11.3 mm / inhibition area on Epidermophyton sp. - 11.3 mm / inhibition area on Trichophyton sp.) (P <0.001). MIC (Minimal inhibition concentration) breakpoints that strengthen the disk diffusion method are 50-100 μL (4500– 9000 μg in 10 mL) as the smallest value that inhibits the growth of bacteria, yeasts, dermatophyta. The antimicrobial compound can be of great advantage to illuminate future studies in this area. The polymer used in the study will provide a promising new addition to antimicrobial polymers that fight microorganisms that cause inflammation and fungal infections.

Keywords:

New antimicrobial polymeric compound, Inhibitory effect, Poly (DMAA-co-MMA),

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