Mehmet Akif KILINÇ, Ali RİŞVANLI, Tarık ŞAFAK, Öznur YILMAZ, Burak Fatih YÜKSEL, İbrahim ŞEKER

GEBE İNEKLERDE KAN ASPROSİN DÜZEYLERİNİN BİYOKİMYASAL PARAMETRELERLE İLİŞKİSİ

Bu çalışmada, gebe ve gebe olmayan ineklerde asprosin düzeylerinin tespit edilmesi, bazı biyokimyasal parametreler ile asprosin düzeyleri arasındaki ilişkinin belirlenmesi amaçlanmıştır. Bu amaçla 60 adet Simental ırkı inek, gebe olan (n=30) ve postpartum 15. günde (n=30) gebe olmayan inekler olmak üzere iki gruba ayrıldı ve ineklerden bir kez kan alınarak asprosin düzeyleri ve biyokimyasal parametreler ölçüldü. Ölçülen biyokimyasal parametreler Alkalen fosfataz (ALP), Amilaz, Kreatin kinaz miyokardial band (CK-MB), Kreatin kinaz N-asetil sistein (CK-NAC), Gama glutamil transferaz (GGT), Aspartat Aminotransferaz (AST), Alanin aminotransferaz (ALT), Direkt bilirubin, Total bilirubin , Kalsiyum (Ca), Kolin (CHO), Kreatin, Glikoz, Total protein, Üre, Albümin, Demir, Fosfor, Lacktat dehidrogenaz (LDH-P), High-density lipoprotein kolesterol (HDL-C), Low-density lipoprotein (LDL), Trigliserit, Magnezyum (Mg) ve Unsaturated iron binding capacity (UIBC)’dir. Asprosin düzeyleri ticari ELİSA kitleri kullanılarak, biyokimyasal parametreler ise otoanalizörde ölçüldü. Asprosin düzeyleri ve biyokimyasal parametreler hem grup için hem de gruplar arası karşılaştırılarak sonuçlar değerlendirildi. Elde edilen veriler ışığında gebe olmayan ineklerin direkt biluribin ve total biluribin miktarı gebe ineklerden yüksek, CHO, HDL-C, LDL ve asprosin miktarları ise düşük bulundu. Gebe ineklerin asprosin ile biyokimyasal parametreleri karşılaştırıldığında ise asprosin ile albümin, direkt biluribin, total biluribin ve kreatin miktarları arasında pozitif yönde korelasyon bulundu. Gebe olmayan ineklerde ise asprosin ile amilaz ve fosfor arasında negatif korelasyon, asprosin ile kreatin ve glikoz arasında pozitif yönde korelasyon gözlendi. Sonuç olarak, gebe ineklerde asprosin düzeylerinin daha yüksek olduğu ve bu hormonla biyokimyasal parametreler arasında negatif veya pozitif yönde korelasyonlar olabileceği kanaatine varıldı.

Anahtar Kelimeler:

İnek, asprosin, biyokimyasal parametreler

THE RELATIONSHIP OF BLOOD ASPROSIN LEVELS AND BIOCHEMICAL PARAMETERS IN PREGNANT COWS

In this study, it was aimed to determine asprosin levels in pregnant and non-pregnant cows and to determine the relationship between some biochemical parameters and asprosin levels. For this purpose, 60 Simmental cows were divided into two groups as pregnant (n=30) and non-pregnant cows on the postpartum 15th day (n=30), and asprosin levels and biochemical parameters were measured by taking blood from the cows once. Measured biochemical parameters Alkaline phosphatase (ALP), Amylase, Creatine kinase myocardial band (CK-MB), Creatine kinase N-acetyl cysteine (CK-NAC), Gamma glutamyl transferase (GGT), Aspartate Aminotransferase (AST), Alanine aminotransferase (ALT) ), Direct bilirubin, Total bilirubin , Calcium (Ca), Choline (CHO), Creatine, Glucose, Total protein, Urea, Albumin, Iron, Phosphorus, Lacktate dehydrogenase (LDH-P), High-density lipoprotein cholesterol (HDL-C) ), Low-density lipoprotein (LDL), Triglyceride, Magnesium (Mg) and Unsaturated iron binding capacity (UIBC). Asprosin levels were measured using commercial ELISA kits and biochemical parameters were measured in an autoanalyzer. Asprosin levels and biochemical parameters were compared both for and between groups and the results were evaluated. As a result of the data obtained, the amount of direct bilirubin and total bilirubin in non-pregnant cows was found to be higher than that of pregnant cows, while the amounts of CHO, HDL-C, LDL and asprosin were found to be lower. When asprosin and biochemical parameters of pregnant cows were compared, a positive correlation was found between asprosin and albumin, direct bilirubin, total bilirubin and creatinine levels. In non-pregnant cows, there was a negative correlation between asprosin and amylase and phosphorus, and a positive correlation between asprosin and creatine and glucose. As a result, it was concluded that asprosin levels are higher in pregnant cows and there may be negative or positive correlations between this hormone and biochemical parameters.

Keywords:

Cow, Asprosin, Biochemical parameters,

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