Necla Gürbüz, Sema Uğuralp, Feral Öztürk, Hale Kırımlıoğlu

Hipospadias Hastalarının Sünnet Derisinde Epidermal Büyüme Faktörü Reseptörünün Ekspresyonu

Amaç: Hipospadiasın etyolojisi genellikle belirsiz ve multifaktöriyeldir. Epidermal Büyüme Faktörü (EGF, Epidermal Growth Factor) akciğer ve barsak matürasyonunu hızlandırır, hücre proliferasyonunu kolaylaştırır, doku farklılaşmasını ve onkojenezi düzenler, epitel yüzeylerinin normal rejenerasyonu ve iyileşmesinde çok önemli rol oynar. Periüretral deride yetersiz EGF ekspresyonu bildirilmiştir. Çalışmamızın amacı hipospadiaslı çocukların sünnet derisindeki ve sünnet olan çocukların sünnet derisindeki EGFR ekspresyonlarını karşılaştırmaktı. Gereç ve Yöntem: Yedi sağlıklı çocuğun (sünnet sırasında alınmıştır) ve 7 hipospadiaslı çocuğun sünnet derileri epidermal büyüme faktörü reseptörü (EGFR) ekspresyonunun tespiti için boyanmıştır. Bulgular: Gruplar arasında sünnet derilerinin epidermisinde, düz kasında, stromasında, kan damarlarında ve fibroblastlarındaki EGFR ekspresyonu açısından anlamlı fark yoktu (p>0.05). Ancak hipospadias grubunda mast hücrelerinin sayısı artmıştı (p

Anahtar Kelimeler:

Hipospadias, EGF, EGFR, Mast Hücresi

Epidermal Growth Factor Receptor Expression in the Foreskin of Hypospadias Patients

Objectives: The etiology of hypospadias is often obscured and multifactorial. Epidermal growth factor (EGF) accelerates lung and intestinal maturation, promotes cell proliferation, regulates tissue differentiation, and modulates organogenesis and it plays an essential role in the normal regeneration and healing of epithelial surfaces. Inadequate EGFR expression has been reported in periüretral skin.The aim of our study is to compare the EGFR expression in the dorsal foreskin of children with hypospadias and in foreskin of children undergoing circumcision.Materials and Methods: The foreskins of 7 healthy children (obtained during circumcision) and 7 children with hypospadias were stained for epidermal growth factor receptor (EGFR) expression.Results: The EGFR expression in the epidermis, smooth muscle, stroma, blood vessels, and fibroblasts of prepuces were not significantly different between the groups (p>0,05). But the number of the mast cells were increased in the hypospadias group (p<0,05).Discussion: EGFR expression did not differ between the dorsal foreskins of children with hypospadias and foreskins of children undergoing circumcision. But the numbers of mast cells were increased in the foreskins of children with hypospadias. Further cellular studies are required to uncover the mechanism underlying the increase in mast cell number.

Keywords:

Hypospadias, EGF, EGFR, Mast Cell,

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