Saliha Şahin, Gülçin Tezcan, Cevdet Demir, Berrin Tunca, Gülşah Çeçener, Ünal Egeli

Prunella vulgaris L. VE Prunella grandiflora L.’DEN SAFLAŞTIRILAN ROSMARİNİK ASİTİN FARKLI TÜMÖR HÜCRELERİ ÜZERİNDEKİ SİTOTOKSİK AKTİVİTESİ

Bu çalışmada rosmarinik asit bileşiği, Prunella grandiflora L. ve P. vulgaris L. türlerinden elde edilen etanol ekstraktlarından saflaştırılmıştır. Saflaştırma işlemi sırasında elde edilen metanol fraksiyonlarının toplam fenol içeriği Folin-Ciocalteu yöntemi ile belirlenmiştir. Prunella L. türlerinden saflaştırılan rosmarinik asitin farklı kanser hücreleri üzerinde WST-1 (Roche Applied Sciences, Mannheim, Almanya) yöntemiyle sitotoksik doz çalışmaları yapılmıştır. Buna göre pankreas (PANC-1), prostat (PC-3), kolon (HT-29) ve meme (MDA-MB 436) kanserleri ile GBM (T98G) hücre hatları ve lenf dokularında 10-60ng arasında sitotoksik doz belirlenmiştir. Sitotoksik doz belirleme çalışmaları için 24 ve 48 saat inkübasyon süreleri çalışılmıştır. 48 saat inkübasyon süresi sonunda PC3 hücre hattı için 50ng ve PANC-1, HT-29, MDA-MB 436 ve T98G hücre hatları için 60ng rosmarinik asit uygulamasında antiproliferatif etki gözlenmiştir. Sağlıklı hücrelerde rosmarinik asitin sitotoksik etkisi gözlenmemiştir.

Anahtar Kelimeler:

Prunella L., izolasyon, rosmarinic asit, antiproliferatif, sitotoksik aktivite

CYTOTOXIC ACTIVITY OF ROSMARINIC ACID ISOLATED FROM Prunella vulgaris L. AND Prunella grandiflora L. IN DIFFERENT TUMOR CELLS

Rosmarinic acid was isolated from ethanol extractions obtained from Prunella grandiflora L. and P. vulgaris L. The total phenolic content of ethanol fractions during isolation was determined by the Folin-Ciocateu method. The cytotoxic doses of the isolated rosmarinic acid were determined by WST-1 (Roche Applied Sciences, Mannheim, Germany) cell proliferation assay. A 10-60ng cytotoxic dose was determined for pancreas (PANC-1), prostate (PC-3), colon (HT-29) and breast (MDA-MB 436) cancers and GBM (T98G) cell lines and lymphatic tissues. 24 and 48h incubation periods were applied during dose determinations. An antiproliferative effect was observed at the end of 48h incubation period with 50ng rosmaniric acid treatment in PC3 cell line and with 60ng treatment in PANC-1, HT-29, MDA-MB 436 and T98G cell lines. No cytotoxic activity of rosmarinic acid was observed in non-tumor cells

Keywords:

Prunella L., isolation, rosmarinic acid, antiproliferative, cytotoxic activity,

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