Poliglutamik asit (PGA) yüksek üretim maliyeti olan, suda çözünen ve biyobozunabilir bir polimerdir. Bu çalışmada, fizıbıl PGA üretimi için tavuk tüyü hidrolizatları (TH) fermantasyon substratı olarak kullanılmıştır. Keratinaz enzimi eldesi için, Streptomyces pactum DSM 40530 ile doğal tavuk tüyleri fermente (30L) edilmiştir. Fermantasyon çözeltisindeki enzim aktivitesi, çapraz akışlı filtrasyon ünitesi kullanılarak 8,75 kat deriştirilmiştir. Enzim aktivitesi (8×103UL-1gün-1), gram tavuk tüyü başına %75’lik bozunma için optimum değer olarak belirlenmiştir. PGA üretimi için Bacillus licheniformis 9945a kullanılmış ve 40g/L TH kullanılarak farklı kompozisyonlarda besiyeri çözeltileri hazırlanmıştır. Besiyeri-E bileşenleri olan L-glutamat, sodyum sitrat ve gliserol kullanılarak dört farklı kompozisyonda besiyeri hazırlanmıştır. Bunlar arasında en fazla PGA ve kuru hücre maddesi (KHM) üretimi, glutamik asit içermeyen bir numaralı kültürde, sırasıyla 5,4±0,4 and 8,6±0,5g/L olarak gerçekleşmiştir. Glutamik asitin yanı sıra sitrik asitin de olmadığı iki numaralı kültürde PGA ve KHM değerleri sırasıyla 3,2±0,2 ve 7,8±0,4g/L olurken, sadece TH’nin substrat olarak kullanıldığı üç numaralı kültürde yalnızca 1,9±0,2 ve 4,2+0,4g/L değerleri elde edilmiştir. Sadece gliserolün kullanılmadığı dört numaralı kültürde ise PGA ve KHM değerleri bir miktar yükselmiş ve sırasıyla 2,3±0,2 ve 5,5±0,3g/L değerlerini almıştır. Yapılan çalışma PGA üretiminin TH kullanılarak yapıldığı İlk çalışma olmuştur.

BIOPRODUCTION OF γ-POLY(GLUTAMIC ACID) USING FEATHER HYDROLYSATE AS A FERMENTATION SUBSTRATE

Polyglutamic acid (PGA) is water-soluble and biodegradable polymer with high production cost. For feasible PGA production, feather hydrolysate (FH) was used as fermentation substrate. 30L fermentation of native feather was realized to obtain keratinase enzyme using Streptomyces pactum DSM 40530. Fermentation broth was concentrated by cross-flow filtration where the enzyme activity increased by 8.75-fold and 8×103UL-1d-1 of enzyme activity was the optimum for achieving 75% degradation per gram of feather. 40g/L of FH was used with different media compositions to produce PGA using Bacillus licheniformis 9945a. Among four different cultivation where L-glutamate, tri-sodium citrate and glycerol were used as the constituents of Medium E, highest yields of γ-PGA and cell dry matter (CDM) were obtained from cultivation-1, at 5.4±0.4 and 8.6±0.5g/L, respectively, despite the culture media did not contain glutamic acid. In cultivation-2, which was not only missing glutamate but also citrate, the γ-PGA and CDM yielded 3.2±0.2 and 7.8±0.4g/L, respectively whereas it was only 1.9±0.2 and 4.2+0.4g/L when FH was used as the sole substrate in cultivation-3. When cultivation-4 was adopted where only glycerol was missing, the γ-PGA and CDM yields slightly increased to 2.3±0.2 and 5.5±0.3g/L, respectively. This is the first study that achieved the production of γ-PGA from FH.

Keywords:

γ-Poly(glutamic acid), γ-PGA, feather hydrolysate, keratinolytic activity feather,

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Trakya University Journal of Natural Sciences-Cover

ISSN: 2147-0294
Yayın Aralığı: Yılda 2 Sayı
Başlangıç: 2000
Yayıncı: Trakya Üniversitesi

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