Orhan Veli DOĞAN, Alp DOLGUN, Ertan YÜCEL, Selçuk Nazmiye KAPISIZ, Hasan Fahri KAPISIZ

Otolog perikard yamalarının gluteraldehid ile fiksasyonu

Amaç: Çalışmamızın amacı implantasyondan hemen önce ameliyathanede uygulanabilen bir fiksasyon metodu ile perikardial yamaların gerilme kuvveti ve elastikiyetlerini ölçmektir. Hastalar ve Yöntemler: Koroner bypass ameliyatı geçiren 50-70 yaşları arasındaki 60 hastadan alınan perikard yamaları çalışılmıştır. Yatay ve dikey yönde kesilen perikard yamaları %0.5, %1, %2 konsantrasyonlarındaki gluteraldehid solüsyonu ile 5, 10 20 dakika süreyle muamele edilmiştir. Perikardial kesitler uniaksial germe testine tabi tutulmuştur. Kalınlık, maksimum germe kuvveti, maksimum uzama, kopma kuvveti, kopma uzaması farklı fiksasyon grupları arasında karşılaştırılmıştır.Bulgular: Maksimum germe kuvveti, maksimum kopma kuvveti, maksimum uzama ve kopma uzaması %2 konsantrasyonundaki gluteraldehid solüsyonu ile beş dakika muamele edilen perikard yamalarında en yüksek bulunmuştur.Sonuç: Yüzde ikilik gluteraldehid solüsyonunda beş dakika süreyle muamele edilen perikardial kesitlerde uniaksial germe testi ile daha yüksek germe kuvveti ve uzaması elde edilmiştir. Klinik uygulamaya konmadan önce benzer çalışmaların biaksial germe testi ile yapılması önerilmektedir.

Glutaraldehyde fixation of autologous pericardial patches

Objectives: The aim of our study is to measure ultimate tensile strength and elongation of pericardial patches prepared by a fixation method that can be applied just in the operating room before its implantation. Patients and Methods: Pericardial patches taken from 60 patients of 50-70 years of age who underwent surgery for coronary artery bypass were studied. Pericardial patches cut in horizontal and vertical directions were treated with 0.5%, 1%, 2% concentrations of glutaraldehyde, each for 5, 10, 20-minute durations. Uniaxial tensile test was applied on pericardial samples. Thickness, tensile strength at yield, elongation at yield, tensile strength at break, elongation at break were compared between different fixation groups.Results: Ultimate tensile strength at yield and at break, elongation at yield and at break were found to be highest in pericardial patches treated with 2% glutaraldehyde concentration for five-minute duration.Conclusion: Fixation of pericardial samples by 2% glutaraldehyde concentration for five-minute duration resulted in higher tensile strength and elongation with uniaxial stretching. Further similar studies with biaxial stretching is suggested before application in clinical practice.

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