Pınar SEYMEN, Erman AYTAÇ, Feray BÖLÜKBAŞI, Fatih DEMİR, Habibe GENÇ, Hafize UZUN, Tuncay ALTUĞ, HAKKI OKTAY SEYMEN

Effects of darbepoetin alpha on brain tissue oxidative stress in experimental ethanol administration

Amaç: Eritropoetin analoğu darbepoetin alfa, yarı ömrü uzun ve in vivo yüksek aktiviteye sahip bir moleküldür. Alkolün etkili olduğu organizmada darbepoetinin etkisi henüz ortaya konulmamıştır. Bu çalışmada, deneysel etanol uygulamasında darbepoetin alfanın beyin dokusu oksidan/antioksidan denge ve nitrik oksit seviyesi üzerine etkilerini araştırdık. Hastalar ve Yöntemler: Kırk dört erişkin erkek Wistar Albino sıçan raslantısal olarak gruplara ayrıldı: %0.9 NaCl uygulanan grup (S) (n=10), %0.9 NaCl ve darbepoetin verilen grup (D) (10 μg /kg) (n=10), deneysel etanol uygulaması yapılan [2.5 g/kg (2.6 ml/kg) 2-saatlik aralıklar ile iki defa] grup (E) (n=12), etanol uygulanan ve darbepoetin verilen grup (ED) (n=12). Bulgular: ED grubu malondialdehit (MDA) değerleri E grubundan anlamlı olarak düşüktü (p

Anahtar Kelimeler:

Etanol, Oksidatif stres, Nitrik oksit, Sıçan, Wistar, Modeller, hayvan

Deneysel etanol uygulamasında darbepoetin alfanın beyin dokusu oksidatif stresi üzerine etkileri

Objectives: The hyperglycosylated erythropoietin analogue darbepoetin alpha (α) has longer half-life and higher in vivo activity. There is no data about the effects of darbepoetin-α on ethanol-induced oxidative stress. In this study, we investigated the effects of darbepoetin-α on brain tissue oxidant/antioxidant status and nitric oxide levels in experimental ethanol administration. Patients and Methods: Forty-four adult male Wistar albino rats were randomly divided into groups: salinetreated group (S) (n=10), saline and darbepoetintreated group (D) (10 μg/kg) (n=10), experimental ethanol-administered [2.5 g/kg (2.6 ml/kg) twice at 2-hr intervals] group (E) (n=12), ethanol-administered and darbepoetin-treated group (ED) (n=12). Results: Malondialdehyde (MDA) levels of ED group were significantly lower than E group (p<0.05). Glutathione (GSH) levels of ED group were significantly higher than E group (p<0.001). NO levels of ED group were significantly lower than E group (p<0.001). Conclusion: We have observed that darbepoetin-α decreases oxidants and increases antioxidants against ethanol-induced oxidative stress. Darbepoetin-α is protective in ethanol-induced organism via its antioxidant activity.

Keywords:

Ethanol, Oxidative Stress, Nitric Oxide, Rats, Wistar, Models, Animal,

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