In this study the selective determination of tannic acid (TA) was performed using a glassy carbon (GC) electrode modified with 1-Benzoyl-3-(pyrrolidine) thiourea (PrTu). PrTuwas deposited on the GC electrode in nonaqueous media by cyclic voltammetry. PrTu-GC electrode demonstrated an electrocatalytical effect on the oxidation of TA using differential pulse voltammetry (DPV) method in 0.1 M Britton Robinson (BR) buffer solution of pH 2. Under optimum conditions linear calibration graphs were obtained over the TA concentration range 2.0×10−6 to 4.2×10−5 M. The detection of limit (LOD) was found as 0.6 µM. The proposed electrode was applied to the determination of TA in tea sample with satisfactory results.