Üzeyir GÖK, Erol KELEŞ, Aykut ÖZDARENDELİ, Yasemin BULUT, Bengü ÇOBANOĞLU

Skuamöz hücreli larenks kanseri ve vokal kord nodüllü hastaların cerrahi doku örneklerinde polimeraz zincir reaksiyonuyla Epstein-Barr virüsü DNA'sı araştırması

Amaç: Larenjeal skuamöz hücreli karsinom ve vokal kordnodüllerine ait cerrahi örneklerde Ebstein-Barr virüsüarafltırıldı. Hastalar ve Yöntemler: Rastgele seçim yöntemiyle skuamöz hücreli larenks kanseri olan 22 hastanın 20 erkek,2 kadın; ort. yafl 52 ve vokal kord nodülü olan 17 hastanın 14 erkek, 3 kadın; ort. yafl 38 formalin ve parafindebekletilen cerrahi doku örnekleri polimeraz zincir reaksiyonu yöntemiyle incelendi. Olgular, hastalığın yerleflim yeri,sigara içme alıflkanlığı, süresi ve miktarı ve EBV DNAvarlığı açısından değerlendirildi. Bulgular: Larenks kanserli olguların 21’inde, vokal kordnodüllü olguların dokuzunda sigara içme öyküsü vardı.Polimeraz zincir reaksiyonu ile, kanserli olguların 11’inde %50 , vokal kord nodüllü olguların yedisinde %41.2 EBV-DNA pozitifliği saptandı. Gruplar içinde EBV-DNAvarlığı, sigara içme alıflkanlığı, süresi, bir günde tüketilensigara sayısı, hastalığın yerleflimi ve tümör evresi bakımından anlamlı farklılık görülmedi p > 0 . 0 5 .Sonuç: Bulgularımız, larenjeal skuamöz hücreli karsinompatogenezi ile EBV arasında doğrudan iliflki olmadığınıgöstermektedir; ama larengeal proliferatif hastalıkların etyolojisindeki rolü daha fazla arafltırma gerektirmektedir

Anahtar Kelimeler:

Karsinom, skuamöz hücreli/viroloji, DNA, neoplazi, DNA, viral, Herpesvirüs 4, insan/izolasyon ve purifikasyon, larenks neoplazileri/immünoloji, polimeraz zincir reaksiyonu/yöntem, vokal kord/immünoloji

Detection of Epstein-Barr virüs DNA by polymerase chain reaction in surgical specimens of patients with squamous celi carcinoma of the larynx and vocal cord nodules

Objectives: We investigated the incidence of Epstein- Barr virüs EBV infection in surgical specimens of squa- mous celi carcinoma of the larynx and vocal cord nodules.Patients and Methods: Formalin-fixed, paraffin-embedded tissue samples randomly selected from 22 patients with squamous celi carcinoma of the larynx 20 males, 2females; mean age 52 years and from 17 patients with vocal cord nodules 14 males, 3 females; mean age 38 years were examined by the polymerase chain reaction. The patients were assessed in terms of localization of the disease, smoking habits, duration of smoking, and the presence or absence of EBV DNA.Results : Twenty-one patients with laryngeal carcinoma and nine patients with vocal cord nodules were smokers.Polymerase chain reaction showed EBV DNA in 11 patients 50% with laryngeal carcinoma and in seven patients 41.2% with vocal cord nodules. No significant differences were found with respect to EBV DNA positivity, smoking habits, smoking period, the number of cigarettes consumed daily, localization of disease, and tumor stage p>0.05 .Conclusion: Epstein-Barr virüs does not seem to be directly associated with the pathogenesis of laryngeal squamous celi carcinoma, but its role in the etiology of laryngeal proliferative diseases needs more elucidation.

Keywords:

Carcinoma, squamous cell/virology, DNA, neoplasm, DNA, viral, Herpesvirus 4, human/isolation & purification, laryngeal neoplasms/immunology, polymerase chain reaction/methods, vocal cords/immunology,

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Rafferty MA, Fenton JE, Jones AS. The history, aetiolo- gy and epidemiology of laryngeal carcinoma. Clin Otolaryngol 2001;26:442-6.
Lennette ET. Epstein-Barr virus. In: Muray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, editors. Manual of clinical microbiology. 6th ed. Washington DC: ASM Press; 1995. p. 905-10.
Miller G. Epstein-Barr virus. In: Fields BN, Knipe DM, editors. Virology. 1st ed. New York: Raven Press; 1990. p. 1921-58.
Clark DR, Young LS, Proops DW. Epstein-Barr virus and epithelial cells: implications for the otolaryngolo- gist. J Otolaryngol 1988;17:1-3.
Straus SE, Cohen JI, Tosato G, Meier J. NIH conference. Epstein-Barr virus infections: biology, pathogenesis, and management. Ann Intern Med 1993;118:45-58.
Shu CH, Chang YS, Liang CL, Liu ST, Lin CZ, Chang P. Distribution of type A and type B EBV in normal individuals and patients with head and neck carcino- mas in Taiwan. J Virol Methods 1992;38:123-30.
Brichacek B, Hirsch I, Sibl O, Vilikusova E, Vonka V. Association of some supraglottic laryngeal carcinomas with EB virus. Int J Cancer 1983;32:193-7.
Shimizu H, Burns CJ. Extraction of nucleic acids: sam- ple preparation from paraffin-embedded tissues. In: Innis MA, editor. PCR strategies: key concepts for PCR. San Diego: Academic Press; 1990. p. 32-8.
Golusinski W, Olofsson J, Szmeja Z, Biczysko W, Krygier-Stojalowska A, Kulczynski B. A comprehen- sive analysis of selected diagnostic methods with respect to their usefulness in evaluating the biology of neoplastic cells in patients with laryngeal cancer. Eur Arch Otorhinolaryngol 1999;256:306-11.
Henle W, Henle GE, Horwitz CA. Epstein-Barr virus specific diagnostic tests in infectious mononucleosis. Hum Pathol 1974;5:551-65.
Kiaris H, Ergazaki M, Segas J, Spandidos DA. Detection of Epstein-Barr virus genome in squamous cell carc i n o- mas of the larynx. Int J Biol Markers 1995;10:211 - 5 .
Callaghan DJ, Conner BR, Strauss M. Epstein-Barr virus antibody titers in cancer of the head and neck. Arch Otolaryngol 1983;109:781-4.
Murono S, Yoshizaki T, Tanaka S, Takeshita H, Park CS, Furukawa M. Detection of Epstein-Barr virus in nasopharyngeal carcinoma by in situ hybridization and polymerase chain reaction. Laryngoscope 1997; 107:523-6.
Mariette X, Gozlan J, Clerc D, Bisson M, Morinet F. Detection of Epstein-Barr virus DNA by in situ hybridization and polymerase chain reaction in sali- vary gland biopsy specimens from patients with Sjogren's syndrome. Am J Med 1991;90:286-94.
Leyvraz S, Henle W, Chahinian AP, Perlmann C, Klein G, Gordon RE, et al. Association of Epstein-Barr virus with thymic carcinoma. N Engl J Med 1985;312:1296-9.
Brichacek B, Hirsch I, Sibl O, Vilikusova E, Vonka V. Presence of Epstein-Barr virus DNA in carcinomas of the palatine tonsil. J Natl Cancer Inst 1984;72:809-15.
Khabie N, Savva A, Kasperbauer JL, McGovern R, Gostout B, Strome SE. Epstein-Barr virus DNA is not increased in tonsillar carcinoma. Laryngoscope 2001; 111:811-4.
Bulut F, Saydam L, Öncel S, Kalcıoğlu MT, Aydın A, Kızılay A, ve ark. Bafl-boyun epidermoid kanseri ile Epstein-Barr virüsü arasındaki iliflki. KBB Klinikleri 2001;3:12-6.
Carlin MF, Saniga RD, Dennis N. Relationship between academic placement and perception of abuse of the voice. Percept Mot Skills 1990;71:299-304.
Kay NJ. Vocal nodules in children-aetiology and man- agement. J Laryngol Otol 1982;96:731-6.