Pelin KOÇDOR, Işıl ÇOKER, Ümit BAYOL, Deniz ALTINEL, Duygu ÜNÜVAR PURCU, Suat KAPTANER, Orhan Gazi YİĞİTBAŞI

Larengeal skuamöz hücreli karsinomalarda qRT-PCR kullanılarak VEBF ekspresyonu ölçümü, MDY değerlendirilmesi ve bunların klinikopatolojik faktörlerle ilişkisi

Amaç: Bu çalışmada, larenks skuamöz hücreli kanser LSHK dokularında kantitatif gerçek zamanlı polimeraz zincir reaksiyonu kullanılarak vasküler endotelyal büyüme faktörü VEBF düzeyleri ölçüldü, VEBF, mikrodamar yoğunluğu MDY ve klinikopatolojik faktörler arasındaki ilişki değerlendirildi.Hastalar ve Yöntemler: Eylül 2006 - Temmuz 2008 tarihleri arasında İzmir Tepecik Eğitim ve Araştırma Hastanesi Kulak Burun Boğaz Baş-Boyun Cerrahisi Kliniği’ne başvuran, 27 LSHK hastasına total veya parsiyel cerrahi uygulandı. Yirmi yedi hastanın ikisi, VEBF ekspresyonu olmadığı için çalışmaya dahil edilmedi. Çalışmaya 25 hasta 24 erkek, 1 kadın; ort. yaş 61 yıl; dağılım 43-82 yıl alındı, fakat bu hastaların 10’unda MDY seviyesi belirlenemedi.Bulgular: Amerikan Kanser Komitesi AJCC ’nin 2003 yılında tanımladığı TNM sınıflandırmasına göre; yedi hasta %28 evre 1, altı hasta %24 evre 2, dört hasta %16 evre 3, sekiz hasta %32 evre 4 idi. On üç hasta iyi derece diferansiye G1 , 12 hasta orta derece diferansiye G2 tümöre sahipti. Mikrodamar yoğunluk değerleri belirlenebilen 15 hasta içinde, median değer 48 idi dağılım 13-78; damar / 3.76 mm2 . Vasküler endotelyal büyüme faktörü seviyeleri tespit edilebilen 25 hasta arasında, median değer 0.035 damar / 3.76 mm2 dağılım 0.010-0.127 idi.Sonuç: Klinikopatolojik faktörler ve ayrıca VEBF ve MDY arasında istatistiksel olarak anlamlı bir ilişki bulunamadı. Bizim çalışmamız LSKH’den VEBF salındığını gösterdi

Anahtar Kelimeler:

Larenks skuamöz hücreli kanser, kantitatif gerçek zamanlı polimeraz zincir reaksiyonu, vasküler endotelyal büyüme faktörü

Quantifying the expression of VEGF using qRT-PCR, evaluation of MVD and their correlation with clinicopathological factors in laryngeal squamous cell carcinoma

Objectives: In this study we quantified the expression of vascular endothelial growth factor VEGF using quantitative real-time polymerase chain reaction in laryngeal squamous cell carcinoma LSCC tissues and evaluated the correlation between the level of VEGF and microvessel density MVD , and clinicopathological factors. Patients and Methods: Twenty-seven patients with LSCC undergoing total or partial laryngectomy at the Ear, Nose, and Throat and Head and Neck Surgery Department of the Izmir Tepecik Training and Research Hospital between September 2006 and July 2008. There was no VEGF expression in two patients that were excluded from the study. Twenty-five patients 24 males, 1 female; mean age 61 years; range 43 to 82 years were included in this study, but MVD levels of 10 patients could not be determined. Results: As defined by the 2003 American Joint Committee on Cancer AJCC TNM classification, seven patients 28% were stage 1, six patients 24% were stage 2, four patients 16% were stage 3, and eight patients 32% were stage 4. Thirteen patients 52% had well-differentiated G1 tumors, and twelve had moderately differentiated tumors. Among the 15 patients for whom the MVD was determined, the median value was 48, with a range 13-78; vessels / 3.76 mm2 . Among the 25 patients for whom the VEGF level was determined, the median value was 0.035 vessels / 3.76 mm2 range 0.010-0.127 . Conclusion: We could not find a statistical correlation between clinicopathological factors and either VEGF or MVD. Our study demonstrates that VEGF is expressed by LSCC.

Keywords:

Laryngeal squamous cell carcinoma, quantitative real-time polymerase chain reaction, vascular endothelial growth factor,

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