The Comparison of Sensitivity of Various Methods in The Detection of Olive Tree Viruses
The Comparison of Sensitivity of Various Methods in The Detection of Olive Tree Viruses
To compare the sensivity of different methods used to detect the agents of viral diseases in olive trees, totaly 375 samples of leaves, fruits and flowers were collected from olive trees in Aydın, Balikesir and Izmir provinces and for further studies, 27 samples were selected from 375 ones as a one representative sample from each of counties in these provinces. The existence of 9 viral agents in these samples was checked by RT-PCR while the detection of 4 viruses was done with DAS-ELISA. When the juice from the samples was inoculated to certain test plants, they did not produce any symptoms. It was found that all samples were infected with the any of viruses entitled Arabis mosaic virus (ArMV), Cherry leafroll virus (CLRV), Strawberry latent ringspot virus (SLRSV) and Cucumis mosaic virus (CMV) by RT-PCR while ArMV was present in only 5 samples in DAS-ELISA. Moreover, all samples in this study were free from Olive latent-1 virus (OLV-1), Olive latent-2 virus (OLV-2), Olive latent-3 virus (OLV-3), Olive latent ringspot virus (OLRSV) and Olive leaf yellowing-associated virus (OLYaV) by RT-PCR. The results of RT-PCR showed that the samples from 3 trees having virus-like symptoms were infected with a virus and on the contrary, 24 samples from the trees which were determined to be infected with viruses did not demonstrated any virus symptoms. In conclusion, it was concluded that RT-PCR was the most sensitive method in the detection of the viruses in olive trees at this moment and has the potential usage on the applications of quarantine and certification programs in safety.
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- ISSN: 0378-8024
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 1971
- Yayıncı: Türkiye Fitopatoloji Derneği
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