Molecular Characterization of Rhizoctonia Species and Anastomosis Groups in Barley Production Areas in Ankara Province
Molecular Characterization of Rhizoctonia Species and Anastomosis Groups in Barley Production Areas in Ankara Province
Barley (Hordeum vulgare L.) is an important
cereal crop in terms of human and animal nutrition in the world. Rhizoctonia species are a diverse group
of fungi that damage small-grain cereals by rotting the seedlings, roots and
crowns. In order to determine the root rot diseases, surveys were carried out
in barley fields in 2015 growing season in Ankara. 48 barley and 48 soil samples
were collected from barley fields. As a result of isolation from plant and soil
samples collected from barley fields, 28 Rhizoctonia
isolates belonging to 9 anastomosis groups were obtained. The isolates were identified
based on hyphal, colony morphology, anastomosis reaction with known tester
isolates and rDNA-ITS sequence analysis. Multinucleate (MN) Rhizoctonia isolates were grouped into
five AGs as R. solani AG 2-2, AG 4 HG
II, AG 5, Waitea circinata var. circinata, Waitea circinata var. oryzae and Binucleate (BN) Rhizoctonia isolates were grouped into
four AGs as AG D I (R. cerealis), AG
A, AG E and AG I. As a result of pathogenicity tests, AG 2-2, AG 4 HG II, AG 5,
Waitea circinata var. circinata, Waitea circinata var. oryzae
and BN AG D I (R. cerealis) groups
were found to be pathogen on barley and it was determined that the most
virulent group was AG 4 HG II.
Keywords:
Rhizoctonia spp. anastomosis group, barley,
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- Aroca, A., Raposo, R. 2007. PCR-based stragety to detect and identify species of Phaeoacremonium causing grapevine diseases. Applied and Environmental Microbiology, 73, 2911-2918.
- Bandoni, R.J. 1979. Safranin O as a rapid stain for fungi. Mycologia 71:873–874.
- Cobos, R., Martin, M.T. 2008. Molecular characterisation of Phaeomoniella chlamydospora isolated from grapevines in Castilla y León (Spain). Phytopathol. Mediterr., 47, 20–27.
- Demirci, E. 1998. Rhizoctonia species and anastomosis groups isolated from barley and wheat in Erzurum, Turkey, Plant Pathology, 47(1): 10-15.
- González, D., Cubeta M.A., Vilgalys R. 2006. Phylogenetic utility of indels within ribosomal DNA and _-tubulin sequences from fungi in the Rhizoctonia solani species complex. Molecular Phylogenetics and Evolution 40 (2006) 459–470.
- Sharon, M., Kuninaga, S., Hyakumachi, M., Naito, S. and Sneh, B. 2008. Classification of Rhizoctonia spp. using rDNA-ITS sequence analysissupports the genetic basis of the classical anastomosis grouping. Mycoscience, 49, 93–114.
- Sneh, B., Jabaji-Hare, S., Neate, S. and Dijst, G., 1996. Rhizoctonia species: Taxonomy, molecular biology, ecology, pathology and diseases control. 1-559.
- Tewoldemedhin, Y.T., Lamprecht, S.C., McLeod, A. and Mazzola, M. 2006. Characterization of Rhizoctonia spp. Recovered from crop plants used in rotational cropping systems in the Western Cape Province of South Africa. Plant Disease, 90(11): 1399-1406.
- Kim, D.S., Cook, R. J., and Weller, D.M. 1997. Bacillus sp. L324-92 for biological control of three root diseases of wheat grown with reduced tillage. Phytopathology, 87: 551-558.
- Li, Q.X. and Xia, Z.J. 1988. Study on the biological characters of Rhizoctonia isolated from some crops in Jiangsu. Journal of Jiangsu Agricultural College, 9(3): 23-26.
- Xia, Z.J. and Li, Q.X. 1989. Preliminary study on aetiology of sharp eyespot in wheat and barley in Jiangsu, China. Acta Phytopathologica Sinica, 19(3): 135-139.
- Tomaso-Peterson, M. and Trevathan, L.E. 2007. Characterization of Rhizoctonia-like fungi isolated from agronomic crops and turfgrasses in Mississippi. Plant Disease, 91(3): 260-265.
- Ogoshi, A., Cook, R.J. and Bassett, E.N. 1990. Rhizoctonia species and anastomosis groups causing root rot of wheat and barley in the Pacific Northwest. Phytopathology, 80(9),785-788.
- Schroeder, K.L., Shetty, K.K. and Paulitz, T.C. 2011. Survey of Rhizoctonia spp. from wheat soils in the U.S. and determination of pathogenicity on wheat and barley. Phytopathology, 101: 161s.
- Carling, D.E., Kuninaga, S. and Brainard, K.A. 2002. Hyphal anastomosis reactions, rDNA-internal transcribed spacer sequences, and virulence levels among subsets of Rhizoctonia solani anastomosis group-2 (AG-2) and AG-BI. Phytopathology 92:43–50.
- Hyakumachi, M., Priyatmojo, A., Kubota, M. and Fukui, H. 2005. New Anastomosis Groups, AG-T and AG-U,of Binucleate Rhizoctonia spp. Causing Root and Stem Rot of Cut-Flower and Miniature Roses. Phytopathology, 95(7): 784-792.
- Priyatmojo, A., Yamauchi, R., Kageyama, K. and Hyakumachi, M. 2001. Grouping of Binucleate Rhizoctonia Anastomosis Group D (AG-D) Isolates into Subgroups I and II Based on Whole-Cell Fatty Acid Compositions. J. Phytopathology, 149, 421- 426.
- Salazar, O., Julian M.C., Rubio V. 2000. Primer based on specific rDNA-ITS sequences for PCR detection of Rhizoctonia solani, R. solani AG 2 subgroups and ecological types, and binucleate Rhizoctonia. Mycol Res 104:281–285.
- Kronland, W.C., Stanghellini, M.E. 1988. Clean slide technique for the observation of anastomosis and nuclear condition of Rhizoctonia solani. Phytopathology 78:820–822.
- Paulitz, T.C., Smith, J.D. and Kidwell, K.K. 2003. Virulence of Rhizoctonia oryzae on wheat and barley cultuvars from the Pacific Northwest. Plant disease, 87(1): 51-55.
- Juan-Abgona, R.V., Katsuno, N., Kageyama, K., and Hyakumachi, M. 1996 Isolation and identification of hypovirulent Rhizoctonia spp. from soil. Plant Pathology, 45 (5): 896-904.
- Ichielevich- Auster, M., Sneh, B., Koltin, Y. and Barash, I. 1985. Suppression of Damping-Off Caused by Rhizoctonia Species by a Nonpathogenic Isolate of R. solani. Phytopathology 75: 1080-1084.
- Chen, C.S. and Chuang, T.Y. 1997. The anastomosis groups of binucleate Rhizoctonia in Taiwanese soils. Plant Pathology Bulletin, 6(4), 153-162.
- Cardoso, J.E. and Echandi, E. 1987a. Biological control of Rhizoctonia root rot of snap bean with binucleate Rhizoctonia-like fungi. Plant Disease, 71:167-170.
- Cardoso, J.E and Echandi, E. 1987b. Nature of protection of bean seedlings from Rhizoctonia root rot by a binucleate Rhizoctonia-like fungus, Phytopathology, 77, 1548-1551.
- Gutiérrez, P. and Torres, H. 1990. Biological control of Rhizoctonia solani with binucleated Rhizoctonia. Fitopatología, 25, 2, 45-50 pp.
- Roberts, F.A. and Sivasithamparam, K. 1986. Identity and pathogenicity of Rhizoctonia spp. associated with bare patch diseases of cereals at field site in Western Australia. Europan Journal of Plant Pathology, 92(5): 185-195.
- Herr, L.J. 1988. Biocontrol of Rhizoctonia crown and root rot of sugar beet by binucleate Rhizoctonia spp. and Laetisaria arvalis, Annals of Applied Biology, 113,107-118.
- Eken, C. and Demirci, E. 2004. Anastomosis groups and pathogenicity of Rhizoctonia solani and binucleate Rhizoctonia isolates from bean in Erzurum, Turkey. Journal of Plant Pathology 86(1): 49-52.
- Kural, İ., Sagır, A. and Tatli, F. 1994. Characterization and Pathogenicity of Anastomosis Groups of Rhizoctonia solani İsolated from Cotton in Southeastern Turkey. 9. Congress of the Mediterranean Phytopathological Union. 18-24, Kuşadası Aydın, Türkiye. p. 117- 120.
- Yıldız, A. and Döken, M.T. 2002. Anastomosis group determination of Rhizoctonia solani Kuhn (Telomorph: Thanatephorus cucumeris) isolates from tomatoes grown in Aydın, Turkey and their disease reaction on various tomato cultivars. Journal of Phytopathology -Phytopathologısch Zeithschrift, 150(10): 526-528.
- ISSN: 0378-8024
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 1971
- Yayıncı: Türkiye Fitopatoloji Derneği