Detection of Viruses in Aegean Region Grapevines
Detection of Viruses in Aegean Region Grapevines
This study was conducted to determine virus diseases on grapes in İzmir
and Manisa provinces in 2007-2010 using serological and molecular methods. In
addition to this,detection and identification of grapevine viruses on samples
from Çanakkale and Denizli in 2009-2010 were performed.To identify of viruses in grapevine production areas in the four provinces,
the samples were collected from plants showing virus symptoms. Sampling was
done 2 times a year in the spring and in the autumn. In the spring, shoot and
leaf samples were taken from annual shoots of plants with virus like symptoms
(yellow mosaic,vein clearing, ringspot, vein banding, fasciations, zigzag
growth, shortening of internodes, abnormal branching). In the autumn, reddish
and rolling downwards leaf samples from annual shoots were collected. Totally
746 samples were taken from plants with virus like symptoms in surveyed area in
2007-2010.
Identification of viruses was performed using serological and molecular
methods. DAS-ELISA test results showed that the plants were infected with GFLV,
GFkV, ArMV, GLRaV 1, 2, 3 and 4-9 viruses. PCR analysis confirmed DAS-ELISA
results. In PCR tests, bands for GFLV, GFkV, ArMV, GLRaV 1, 2, 3 and 4 were
obtained.
Keywords:
Grapevine, grapevine viruses, survey ELISA, PCR,
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- Acheche, H., Fattouch, S., M’hırsı, S., Marzoukı, N., And Marrakchı, M., (1999). Use of Optimised PCR Methods for the Detection of GLRaV 3: A Closterovirus Associated with Grapevine Leafroll in Tunusian Grapevine Plants. Plant Molecular Biology Reporter 17:31-42. Akbaş, B.,Erdiller, G. 1993. Researches on Grapevine Virus Diaseases and Determination of Their İncidences in Ankara. The Journal of Turkish Phytopathology, 22 (2-3): 55-64. Akbaş, B., Kunter, B., İlhan, D. 2007. Occurence and Distribution of Grapevine Leafroll Associated Viruses 1,2,3, and 7 in Turkey. J. Phytopathol. 155:122-124. Akbaş, B., Kunter, B., İlhan, D. 2009. Influence of Leafroll on Local Grapevine Cultivars in Agroecological Conditions of Central Anatolia Region. Horticultural Science, 97-104. Alknownı, R. Rowhanı, A., Daubert, S., Golıno, D. 2004. Partial Characterization of a New Ampelovirus Associated with Grapevine leafroll Disease. J. Plant Pathology. 86, 123-133. Azeri, T., 1983. Ülkemiz Bağcılığında Virus Sorunu ve Virussuz Bağ Üretim Programı. Bornova Zir. Müc. Araş. Enst. Md.lüğü, Yıllık, 1 (1) : 61-69. Azeri, T., Çiçek, Y. 1995. Introdüksiyon ve Klon Seleksiyonuyla Elde Edilmiş Bazı Bağ Çeşitleri ve Anaçlarındaki Virus ve Virus Benzeri Hastalıkların Saptanmasına Yönelik Araştırmalar. VII. Türkiye Fitopatoloji Kongresi, Bildiriler, s.374-377, Adana. Bora,T. Ve Karaca,İ., 1970. Kültür Bitkilerinde Hastalığın ve Zararın Ölçülmesi. E.Ü. Ziraat Fakültesi Yardımcı Ders Kitabı, Yayın No:167, pp:43 Boscia, D. Minafra, A., Martelli, G.P. (1997). Filamentous Viruses of Woody Plants, ed: Monette, 19-28. Bovey, R., 1965. Indentification of Viruses in Clonally Propagated Plants Having One or More Viruses. Proc. Conf. On Virus And Vector On Perennial Hosts With Special Reference to Vitis, 223-227. Buzkan, N., Walker M.A. (2001). İnfekteli Asmalarda Asma Kısa Boğum Virüsünün (GFLV) Güvenilir Teşhisi İçin RNA Ekstraksiyon Yöntemlerinin Kıyaslanması, Türkiye IX. Fitopatoloji Kongresi, 3-8 Eylül 2001, Tekirdağ, 496-505. Buzkan, N.,Karadağ, S. Öztekin, V., Kaya, A., Mınafra, A., Ben-Dov, Y. 2010. First Report of the Occurence of Grapevine Leafroll Associated Virus-5 in Turkish Vineyards. J. Phytopathol. 158: 448-449 Clark, M.F. And Adams, A.N., 1977. Characteristics of the Microplate Method of Enzyme-Linked Immunosorbent Assay For the Detection of Plant Viruses. J. Gen. Virol. 34, 475-483. Çağlayan, K., Gazel, M. H. 1998. Asma Virüslerinin Saptanmasında F (Ab’) 2 Antibadi Fragmentlerinin Kullanımı. Türkiye VIII. Fitopatoloji Kongresi, 21-25 Eylül, Ankara. 158-161 Çığşar, İ. 2002. Güneydoğu Anadolu Bölgesi ve Nevşehir İllerinde Bağlarda Zararlı Virüs ve Virüs Benzeri Hastalıklarının Biyolojik ve Serolojik Yöntemlerle Saptanması ve İki Nepovirüsün Karakterizasyonu. Ç.Ü. Fen Bilimleri Enstitüsü. Doktora Tezi. Pp:111. Dıgıaro, M., Martellı, G.P., Savıno, V. (2000). Phloem-limited Viruses of the Grapevine in the Mediterranean and Near East. Extended Abstracts 13 th ICGV, Adelaide, 75-76. Dovas, C.I., Katıs,N.I.(2003). A Spot Multiplex Nested RT-PCR fort he Simultaneous and Generic Detection of Viruses Involved in the Aetiology of Grapevine Leafroll and Rugose Wood of Grapevine. Journal of Virological, 109, 217-226. Engelbrecht, D.J., 1980. Application of the Enzyme-Linked Immunosorbent Assay Procedure to the Detection of Grapevine Fanleaf Virus. S. Afr. J. Enol. Viticult. 1, 103-106. Erdiller, G., 1982. Kısaboğum Hastalığı Etmeni (Grapevine Fan Leaf Virus Hewitt)’Nin Morfolojik, Serolojik Özellikleri ve Standart Irklarla Karşılaştırılması Üzerinde Araştırmalar. Ankara Üniv. Zir. Fak. Yayınları: 834, Pp 58. Erkan, S., Gümüş, M., Yorgancı, Ü Ve Yoltaş, T, 1994. Sanayi domatesi tohum örneklerinde domates mozaik virüsü ve bakteriyel kanser etmenlerinin bulunma durumunun saptanması üzerinde araştırmalar. Sanayi domatesi Üretimini Geliştirme Projesi Çalışma Raporu. İzmir. pp: 47. Foissac, X., Svanella-Dumas , L., Gentit, P., Dulucq, M.J., Candresse, T., 2001. Polyvalent Detection of Fruit Tree Tricho, Capillo And Foveaviruses By Nested RT_PCR Using Degenerated and İnosine Containing Primers (PDO RT-PCR). 18th International Symposium Virus and Viruslike Diaseases of Fruit Trees, July 9-15 2000, Canterbury, 48. Gonsalves, D., 1979. Detection of Tomato Ringspot Virus İn Grapevines: A Comparison Of Chenopodium Quinoa And Enzyme-Linked Immunosorbent Assay (Elısa). Plant Disease Rep. 63, 962-965. Gugerlı, P. Brugger J.J Nad Bovey R., (1984). L’enroulement De La Vigne; Mise En Evidence De Particules Virales Et Development D’une Methode İmmuno-Enziymatique Pour Le Diagnostic Rapide. Revue Suisse Vitic. Arboric. Hortic. Vol. 16 (5): 299-304. Gürsoy, Y.Z. (1991) . Asma Yaprak Kıvrılma Virusu ( Grapevine Leafroll Virus Tip I Ve Iıı)’Nun Bazı Üzüm Çeşitlerinde Elısa İle Saptanması. Vı. Türkiye Fitapatoloji Kongresi 7-11 Ekim 1991, İzmir, 397-400. Gürsoy, Y.Z., Yorgancı, Ü., Erkan, S. 1988. Horozköy Ve Çevresindeki Bağlarda Görülen Virüs Hastalıkları Üzerinde Ön Çalışmalar. Iıı. Bağcılık Ve Şarapçılık Sempozyumu, Bildiri Özetleri, 1988, Bursa. Habılı, N., Krake, L.R., Barlas, M., Rezaıan, M.A., (1992). Evaluation of Biological Indexing dsRNA Analysis in Grapevine Virus Elemination. Annals Applied Biology 121, 277-281. Hewıtt, W.B., Gıfford, E. M., 1956. Symptoms For Identifying Fanleaf İn Dormant Grapevines. The Bulletin Deparment Of Agriculture State Of California, Vol Xlv, Number 3. Kepsutlu, İ., Özekmekçi, E., Özek, B. Ve Uğur, A.,1962. Ege Bağcılığında Bulaşık Soysuzlaşma (Kısaboğum) Üzerinde Çalışmalar. Tarım Bakanlığı Mesleki Kitaplar Serisi D/35. Köklü, G., Baloğlu, S. 2000. Determination Of Incidence Of Grapevine Leafroll Associated Viruses İn Some Grapevine Varieties Grown İn Thrace Region. The Journal Of Turkish Phytopathology 29 (2-3), 85-94. Malıogka, V.I., Dovas, C.I., Katıs, N.I., 2008. Generic And Species-Specific Detection Of Viruses Belonging To An Evolutionary Distint Lineage Within The Ampelovirus Genus. Journal Of Virological Methods 154, 41-47. Martin, R.R., Eastwell, K.C., Wagner,A., Lamprecht,S., I. E. Tzanetakis, 2005.Survey For Viruses Of Grapevine İn Oregon And Washington. Plant Disease Vol:89 No:7,763-766. Matus, J.T., Vega,A., Loyola, R., Serrano,C., Cabrera, S. Arce-Johnson P. 2008. Phytoplasma And Virus Detection İn Commercial Plantings Of Vitis Vinifera Cv. Merlot Exhibiting Premature Berry Dehydration. Electronic Journal Of Biotechnology, Vol: 11, No.5 Özaslan, M., Yılmaz, M.A., (1994). Virus Diseases Of Grapevine İn Southeastern Anatolian Region İn Türkiye. 9 Th. Congress Of The Mediterranean Phytopathological Union, Kuşadası-Aydın, Türkiye,Pp.425-427. Ramsdell, D.C., Andrews, R.W., Gillet, J.W., Morris, C.E., 1979. A Comparison Between Enzyme-Linked Immunosorbent Assay (Elısa) And Chenopodium Quinoa For Detection Of Peach Rosette Mosaic Virus İn ‘’Concord’’grapevines. Plant Disease Rep. 63. 74-78. Rowhani, A. (1992). Use of F(ab’)2 Antibody Fragment in ELISA For Detection of Grapevine Viruses. Am. J. Enol. Vitic. 43: 38-40. Rowhani, A., Chay,C., Golino, D.A., Folk, B.W., 1993. Development Of A Polymerase Chain Reaction Technique For The Detection Of Grapevine Fanleaf Virus İn Grapevine Tissue. Phytopathology 83 (7), 749-753. Rowhani, Bıardı, L., Jonson, R., Saldarellı, P., Zhang, Y.P., Chın, J. ,Gren, M. (2000). Simplified Sample Preparation Method and one-tube RT-PCR for Grapevine Viruses. Extended Abstracts 13th Meeting of ICVG, Adelaide, Australia. P 148. Shanmugathan, N., Fletcher, G., 1982. Enzyme-Linked Immunosorbent Assay To Detect Fanleaf Virus In Grapevines Grown In Containers. Plant Disease, 66, 704-707. Tekinel, N., Dolar, M. S., Nas, Z., Bilgin, N., Salih, H., Salcan, Y., 1972. Akdeniz Bölgesi Bağlarında Bulaşık Soysuzlaşma (Fanleaf)’Nın Araştırılması. Bitki Koruma Bülteni, 11: (4) 225-246. Turturo C., Pasquale S., Dong Y., Michele D.,Angelantonio M., Vito S., Martelli G.P., 2005.Genetic Variability And Population Structure Ofgrapevine Leafroll-Associated Virus 3 Isolates. Journal Of General Virology, 86, 217-224. TÜİK, 2010. Türkiye İstatistik Kurumu. http://www.tuik.gov.tr. (Erişim Tarihi: 01 Temmuz 2012). Yılmaz, M.A., Yurtmen, M.İ Çiğsar, İ., Özaslan, M., 1997. A Survey Of Grapevine Viruses İn Turkey. 12 Th Meeting Of The Icvg. Extended Abstracts, Lisbon, Portugal, 113.
- ISSN: 0378-8024
- Yayın Aralığı: Yılda 3 Sayı
- Başlangıç: 1971
- Yayıncı: Türkiye Fitopatoloji Derneği