Mehmet ORHAN, Ömer Fırat TURŞUCULAR, Yakup AYKUT, İdris ÇERKEZ

FARKLI SOLVENT SİSTEMLERİ KULLANILARAK POLİKAPROLAKTON/KİTOSAN NANO/MİKRO LİFLERİN ÜRETİMİ VE ANTİBAKTERİYEL ÖZELLİKLERİNİN İNCELENMESİ

Farklı oranlarda karışımı yapılmış kitosan/polikaprolakton (CHI/PCL) nano/mikro lifler elektroçekim metoduyla üretilmiştir. Elektroçekim çözeltileri aseton ve formik asit (FA) olmak üzere iki farklı solvent kullanılarak hazırlanmıştır. Solvent etkisinin üretilen nano/mikro liflerin morfolojileri, kimyasal ve termal özellikleri ve antibakteriyel aktiviteleri üzerindeki etkileri SEM, FTIR, DSC ve ASTM 2149 01 standardına göre antibakteriyellik analizleri yapılarak ölçülmüştür. Elde edilen sonuçlara göre, solvent olarak aseton kullanıldığında kitosanın partikül olarak PCL lifler tarafından kapsüle edildiği ve liflerin antibakteriyellik özelliklerini düşürdüğü gözlemlenmiştir. Diğer taraftan, solvent olarak formik asit kullanıldığında kitosan ve polikaprolaktonun elektroçekim çözeltisi içerisinde tamamen çözündüğü, kapsülasyonun olmadığı, üretilen ağsı yapı içerisinde hem PCL hemde CHI fibriller yapıda bulunduğu ve elde edilen yapıların daha iyi antibakteriyel özelliklerde oldukları tespit edilmiştir.

PREPERATION AND ANTIBACTERIAL INVESTIGATION OF POLYCAPROLACTONE/CHITOSAN NANO/MICRO FIBERS BY USING DIFFERENT SOLVENT SYSTEMS

Chitosan (CHI) blended polycaprolactone (PCL) nano/micro fibers were prepared with different CHI content via electrospinning procedure. Two different solvents, acetone and formic acid (FA) were used to dissolve and blend the polymers before electrospinning process. Effect of the solvent on the electrospinability of the blend, final nano/micro fiber morphologies, chemical and thermal properties and antibacterial activities were investigated with SEM, FTIR, DSC, and ASTM 2149 01 Standard Dynamic Contact Conditions. The results revealed that chitosan particles were encapsulated in the as-spun PCL fibers with using acetone as the solvent resulted in reduced antibacterial activities Contrarily, when FA is used as the solvent, CHI and PCL were dissolved and blended very well, and enhanced antibacterial activities were obtained from as-spun PCL/CHI nano/micro fibers.

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