Oğuz BAYRAKTAR, Güldemet BAŞAL, Gözde GENÇ

A research on the production of silk sericin powders by using spray drying method

Serisin proteini genellikle ipek endüstrisinde atık olarak uzaklaştırılmaktadır. Tekstilde kullanılan çeşitli liflerle de etkileşimli olarak kullanılabilen ipek serisin tozlarının püskürtmeli kurutma yöntemiyle eldesi atık serisinin katma değerli bir ürüne dönüştürülmesini mümkün kılacaktır. Bu çalışmada sulu serisin çözeltileri laboratuvar ölçekli püskürtmeli kurutucu kullanılarak kuru toz haline getirilmiştir. Bu işlem sırasında proses parametrelerinin elde edilen toz serisinin nem içeriği, parça tipi ve aglomerasyon derecesine olan etkileri iki seviyeli üç değişkenli bir deney tasarımı ile incelenmiş ve doğrusal regresyon analizleri yapılmıştır. Proses parametrelerinden kurutma havasının sıcaklığı 120°C ve 160°C , besleme hızı 1.25 x$10^{-4}$ ve 2.5 x $10^{-4} dm^{3}s^{-1}$ ve püskürtmeli kurutucuya beslenen çözeltideki serisin konsantrasyonu ağırlıkça % 10 ve % 30 olarak seçilmiştir. Etken parametreleri bulmak için deney çıktıları istatiksel olarak analiz edilmiştir. Nem içeriğinin, başta kurutma havası olmak üzere üç proses parametresinden de etkilendiği ortaya çıkarılmıştır. Parça büyüklüğü ve şeklinin başlıca besleme hızına, aglomerasyon derecesinin ise nem içeriğine bağlı olduğu bulunmuştur.

Püskürtmeli kurutma yöntemi kullanılarak ipek serisin tozunun üretimi üzerine bir araştırma

Sericin protein is usually discarded as a waste in silk industry. Production of silk sericin powders, which can be incorporated into fibers used in textile industry, by spray drying method will enable value-added utilization of waste sericin. In this study aqueous sericin solutions were used as raw material for the production of dry powders using a lab-scale spray dryer. A linear regression analyses were employed, in addition to experimental design at two levels with three factors for the analysis of three responses: moisture content, particle type and agglomeration degree. The process factors were the drying air temperature (120ºC and 160ºC), the feed rate (1.25x$10^{-4}$ and 2.5x$10^{-4} dm^{3}s^{-1}$, and the concentration of sericin solutions of 10% and 30% (w/w) fed to the spray dryer. The three responses were analyzed statistically to determine the effective parameters and it was concluded that moisture content depended on three factors--drying air temperature being the dominant parameter. Particle size and shape depended mainly on feed rate and agglomeration depended on the moisture content of the product.

PDF

___

1.Duran, K., Ozdemir, D., Namligoz, E.S., 2007, “The Enzymatic Degumming of Silk Fibers”, Tekstil ve Konfeksiyon, Vol:17 (3), pp. 211-216.
2.Zhang, Y.Q., 2002, “Application of Natural Silk Sericin Protein in Biomaterials”, Biotechnol. Adv., vol. 20, pp. 91-100.
3.Wu, J.H., Wang, Z., Xu, S.Y., 2007, “Preparation and Characterization of Sericin Powder Extracted from Silk Industry Wastewater” Food Chemistry, vol 103, pp. 1255-1262.
4.Vaithanomsat, P., Kitpreechavanich, V., 2008, “Sericin Separation from Silk Degumming Wastewater”, Separation and Purification Technology, vol.59, pp. 129-133.
5.Capar, G. Aygun, S. S., Gecit, M. R., 2008, “Treatment of Silk Production Wastewaters by Membrane Processes for Sericin Recovery”, Journal of Membrane Science, vol. 325, pp. 920-931.
6.Fabiani, C., Pizzichini, M., Spadoni M. et al., 1996, “Treatment of Waste Water from Silk Degumming Process for Protein Recovery and Water Reuse”, Desalination, vol. 105, pp.1-9.
7.Altman, G.H. Diaz, F., Jakuba, C. et al., 2003, “Silk-based Biomaterials”, Biomaterials, vol. 24, pp. 401-416.
8.Jin, H.J., Chen, J., Karageorgiou, V. et al., 2004, “Human Bone Marrow Stromal Cell Responses on Electrospun Silk Fibroin Mats”, Biomaterials, vol. 25, pp. 1039-1047.
9.Sarovat S, Sudatis B, Meesilpa P. et al., 2003, “The Use of Sericin as Antioxidant and Antimicrobial For Poluted Air Treatment”, Rev. Adv. Mater. Sci., vol. 5,193-198.
10.Tamada, Y., 1997, “Anticoagulant and Its Production”, Japan Patent 09-227402A.
11.Sasaki, M., Kato, N., Watanabe, H. et al., 2000, “Silk Protein, Sericin, Suppresses Colon Carcinogenesis Induced by 1,2-dimethylhydrazine in Mice, Oncol. Rep., vol. 7, pp. 1049-1052.
12.Sasaki, M., Watanabe, H., Kato, N., 2001, “Supplemental Silk Protein, Sericin, Suppresses Colon Tumorigenesis in 1, 2-dimethylhydrazine-treated Mice by Reducing Oxidative Stress and Cell Proliferation, Biosci. Biotech. Biochem., vol. 65, pp. 2181-2186.
13.Kato, N., Sato, S., Yamanaka, A. et al., 1998, “Silk Protein, Sericin, Inhibits Lipid Peroxidation and Tyrosinase Activity, Biosci. Biotech. Biochem., vol. 62, pp. 145-147.
14.Kongdee, A., Bechtold T. and Teufel, L., 2005, “Modification of Cellulose Fiber with Silk Sericin”, J. Appl. Polym. Sci., vol. 96, pp. 1421-1428.
15.Kongdee, A., Okubayashi, S., Tabata, I. et al., 2007, “Impregnation of Silk Sericin into Polyester Fibers Using Supercritical Carbon Dioxide”, J. Appl. Polym. Sci., vol. 105, pp. 2091-2097.
16.Gao, Y., Cranston, R., 2008, “Recent Advances in Antimicrobial Treatments of Textiles”, Textile Research Journal, vol. 78, pp. 60-72.
17.Sehnal, F., 2008, “Prospects of the Practical Use of Silk Sericins”, Entomological Research, vol. 38, pp. S1–S8.
18.Bogna, G.G., Halina, K., Mariusz, G., 2004, “Polymer Microspheres as Carriers of Antibacterial Properties of Textiles: A Preliminary Study, Fibres and Textiles in Eastern Europe, vol. 12(4), 48.
19.Thiering, R., Dehghani, F., Dillow, A. et al., 2000, “The Influence of Operating Conditions on the Dense Gas Precipitation of Model Proteins”, J. Chem. Technol. Biotech., vol. 75, pp. 29-41.
20.Stahl, K., Claesson, M., Lilliehorn, P. et al., 2002, “The Effect of Process Variables on the Degradation and Physical Properties of Spray Dried Insulin Intended for Inhalation, Int. J. Pharm., vol. 233, pp. 227-237.
21.Irngartinger, M., Camuglia, V., Damm, M. et al., 2004, “Pulmonary Delivery of Therapeutic Peptides via Dry Powder Inhalation: Effects of Micronisation and Manufacturing, Eur. J. Pharm. Biopharm., vol. 58, pp. 7-14.
22.Prinn, K.B., Costantino, H.R. and Tracy, M., 2002, “Statistical Modeling of Protein Spray Drying at the Lab Scale, AAPS Pharm. Sci. Tech., vol. 3, pp. 1-8.
23.Sakagami, M, Kinoshita, W., Sakon, K. et al., 2002, “Mucoadhesive Beclomethasone Microspheres for Powder Inhalation Their Pharmacokinetics and Ppharmacodynamics Evaluation”, J. Control. Release, vol. 80, pp. 207-218.
24.Asada, M., Takahashi, H., Okamoto, H. et al., 2004, “ Theophylline Particle Design Using Chitosan by the Spray Drying”, Int. J. Pharm., vol. 270, pp. 167-174.
25.Lee-Desautes R, 2005, “Theory of van der Waals Forces as Applied to Particlate Materials”, Educ. Reso. for Part. Techn., 051Q-Lee, University of Kentucky.