Adeshina Yahaya ADENKOLA, Mary Talatu ANGANİ

Ascorbic Acid Supplementation Effect on Haematology and Oxidative Stress Parameters of Broiler Chicken during the Hot-Dry Season in Southern

Bu çalışmada sıcak-kuru mevsimde broyler civcivlerin içme sularına askorbik asit (AA) ilavesinin hematolojik ve oksidatif stres parametreleri üzerine etkisi araştırılmıştır. Denemede 60 adet, bir günlük yaşta civcivler kullanılmış ve 5 tekerrürlü, 30 civciv içeren 2 deneme grubuna tesadüf deneme düzeninde yerleştirilmişlerdir. Kontrol grubu olan birinci gruba herhangi bir ilave yapılmamıştır, ikinci deneme grubunun içme sularına 500 mg/lt düzeyinde AA ilave edilmiştir. Denemenin son gününde (58. gün) her alt gruptaki piliçlerin yarısı seçilmiş ve kanat altı venlerinden kan numuneleri alınmıştır. Alınan kanların yarısı hematolojik parametreler için ayrılmış, diğer yarısı da santrifüj edilerek serumları ayrılıp saklanmıştır. Sonuçlar, kontrol grubunda PCV (%24.25 ± 2.80), hemoglobin (8.08 ± 0.83 gm/dl) ve toplam eritrosit sayısı (4.35 ± 1.13 x 106/ l) değerlerinin sırasıyla %31.50 ± 13.13; 10.50 ± 4.38 gm/dl ve 5.94 ± 1.28 x 106/l olan deneme grubundan önemli derecede daha düşük olduğunu göstermiştir. Kontrol grubundan elde edilen malondialdehit düzeyi (2.18 ± 0.22 ng/ml) deneme grubunda kaydedilen değerden (0.93 ± 0.09 ng/ml) önemli derecede daha yüksek iken süperoksit dismutaz, katalaz ve glutasyon peroksidaz değerleri kontrol grubunda deneme grubuna göre önemli derecede daha yüksek bulunmuştur. Sonuç olarak AA uygulaması broyler piliçlerde sıcak stresinin olumsuz etkilerini hafifletmiştir. Bu nedenle sıcak-kuru mevsimlerde sıcak stresinin etkilerini hafifletmek için AA ilavesi önerilmektedir

Askorbik Asit İlavesinin Güney Gine Savana’da Sıcak-Kurak Mevsimde Broylerlerde Hematolojik ve Oksidatif Stres Parametreleri Üzerine Etkileri

This study was done to investigate the effect of inclusion of ascorbic acid (AA) in drinking water of broiler chicken on haematological and oxidative stress parameters during the hot-dry season. Sixty day old chicks were used for the experiment, and were assigned to two dietary treatments with 30 birds per treatment and five replicate in a completely randomized design. Treatment 1 was the control with no supplements, treatment 11 was supplemented with AA at the rate of 500 mg/L in drinking water termed the experimental group. On the last day (58th) of the experiment half of the birds were randomly selected from each replica group and blood sample (2 ml) was obtained through the wing web and divided into 2 parts; one for determination of haematological parameters while the second part was centrifuged and the resultant serum harvested and stored. The results indicated that the values of PCV (24.25 ± 2.80%), haemoglobin (8.08 ± 0.83 gm/dl) and total erythrocyte count (4.35 ± 1.13 x 106/ l) obtained in control were all significantly lower than the recorded corresponding value in the experimental group 31.50 ± 13.13%; 10.50 ± 4.38 gm/dl and 5.94 ± 1.28 x 106/ l) respectively. The recorded values of malondialdehyde obtained in control (2.18 ± 0.22 ng/ml) was significantly higher than the values recorded in experimental group (0.93 ± 0.09) while the values of superoxide dismutase, catalase and glutathione peroxidase were significantly higher in the control than the obtained values experimental group. The fragiligram shifted more to the left in the control than the experimental group and was statistically significant. In conclusion AA administration mitigate the adverse effects brought about by heat stress in broiler chicken; its supplementation is thus recommended during the hot-dry season to alleviate heat stress

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1. Yousef, M.K. 1978. Principle of bioclamatology and adaptation. In Bioclamatology and the adaptation of livestock (ed. HD Johnson), pp 17 29. Elsevier Science Publisher, Amsterdam, The Netherlands
2. Daghir, N.J. 2009. Nutritional strategies to reduce heat stress in broilers and broiler breeders. Lohmann Information, 44: 6-15
3. Bernabucci, U., Lacetera, N., Baumgard, L.H., Rhoads, R.P., Ronchi, B. and Nardone, A. 2010. Metabolic and hormonal acclimation to heat stress in domestic ruminants. The Animasl Consortium. 4(7): 1167-1183.
4. Ayo, J.O., Obidi, J.A. and Rekwot, P.I. 2011. Effects of heat stress on the well-beings, fertility and hatchability of chickens in the northern guinea savannah zone of Nigeria. A Review. ISRN Veterinary Science, Article ID 838606 10 pages
5. Adenkola, A.Y., Ayo, J.O. and Sackey, A.K.B. 2009. Ascorbic acid induced-modulation of rectal temperature in pigs during the harmattan season. Journal of Thermal Animal Biology, 34(3): 152 - 154.
6. Sahin, K., Sahin, N., Onderci, M., Yarahiogu, S and Kucuk, O. 2001. Protective role of supplemental vitamin E on lipid peroxidation, vitamin E, A and some mineral concentrations of broilers reared under heat stress. Veterinary Medicine (czecholovakia), 46:140 - 144.
7. Teeter, R.G., Belay, T., Deyhim, F and Cason, J.J. 2005. Heat stress steals nutrition and genetic potential. Department of Animal Sciences, Stillwater, Oklahoma.
8. Avellini, L., Silverstrelli, M. and Gaitti, A. 1995. Training-induced modifications in some biochemical defenses against free radicals in equine erythrocytes. Veterinary Research Communications 19: 179 - 184.
9. Adenkola, A.Y., Ayo, J.O. 2009a. Effect of road transportation on erythrocyte osmotic fragility of pigs administered ascorbic acid during the harmattan season in Zaria, Nigeria. J. Cell Anim. Biol. 3 (1), 004 - 008.
10. Akinwande, A.I. and Adebule, A.O.A. 2003. Ascorbic acid and beta-carotene alleviate oxidative effect of London Kingsize® cigarette smoke on tissue lipids. Nigeria Journal of Health and Biochemical Science, 2(1): 12 - 15.
11. Adenkola, A.Y. and Onyeberechi, A.S. 2015. Co – adminisration of ascorbic acid and alpha-tocopherol effect on oxidative parameters of rams transported by road for eight hours. Journals of Free Radicals and Antioxidants.142: 399 - 406.
12. Sies, H. 1991. Oxidative stress 11. Oxidants and antioxidants. Academic press London.
13. Maurice, D.V., Lightsey, S.F., Abudabos, A. and Toler, J.E. 2002. Factors affecting ascorbic acid biosynthesis in chickens: III. Effect of dietary fluoride on L-gulonolactone oxidase activity and tissue ascorbic acid (AsA) concentration. J Anim Physiol Anim Nutr 86: 383-388.
14. Holik, V. 2009. Management of laying hens to minimize heat stress. Lohmann Information 44: 16- 29.
15. Adenkola, A.Y. and Ayo, J.O. 2009b. Effect of Ascorbic Acid on Rectal Temperature Fluctuations in Indigenous Turkeys during the Hot-Dry Season. International Journal of Poultry Science 8(5): 457 – 461.
16. Ayo, J.O. and Sinkalu, V.O. 2007. Effect of ascorbic acid on diurnal variations in rectal temperature of Shaver Brown pullets during the hot-dry season. International Journal of Poultry Science, 6(9): 642 - 646.
17. Tauler, P., Aquilo, A., Gimelo, I., Fuentaspina, E., Tur, J.A. and Pons, A. 2003. Influence of vitamin C diet supplementation on endogenous antioxidant defences during exhaustive exercise. European Journal of Physiology, 446: 658 - 664.
18. Oyewale, J.O. 1992. Changes in osmotic resistance of erythrocytes of cattle, pigs rats, and rabbits during variations in temperature and pH. Journal of Veterinary Medicine, A39: 98 - 104.
19. Schalm, O.W., Jain, N.C. and Caroll, E.J. 1975. Textbook of Veterinary Haematology, 2nd Edition, Published by Lea and Febiger, Philadelphia, Pp. 129 - 250.
20. Faulkner, W.R. and King, J.W. 1970. Manual of Clinical Laboratory Procedures. Published by the Chemical Rubber Company, Cleveland, Ohio, 354 pp.
21. Robertson, G.W. and Maxwell, M.H. 1990. Modifed staining techniques for avian blood cells. British Poult Sci, 31: 881-886.
22. Mitchell, M.A., Kettlewell, P.J., Maxwell, M.H. 1992. Indicators of physiological stress in broiler chickens during road transportation. Animal Welfare, 1: 91-103.
23. Draper, H.H. and Hadley M. 1990a. Malondialdehyde determination as index of lipid peroxidation, Methods in Enzymology, 186, 421 - 431.
24. Altuuntas, I., Delias, N., Sutcu, R. 2002. The effects of organophosphate insecticide methidathion on lipid peroxidation and anti-oxidation enzymes in rat erythrocyte: Role of vitamins E and C. Human Experimental Toxicology, 21, 681- 685.
25. Cheesbrough, M. 1991. Medical Laboratory Manual for Tropical Countries. 2nd Edition, Published by Cambridge University Press, Cambridge, England. Pp 481 - 530.
26. Selyansky, V.M. 1975. Microclimate in poultry houses. Kolos Publishing House, Moscow, pp 304 (In Russian).
27. Balnave, D. 2004. Challenges of accurately defining the nutrient requirements of heat-stressed poultry. In: World's Poultry Science Association invited lecture. Poultry Science, 83: 5-14.
28. Igono, M.O., Molokwu, E.C.I. and Aliu, Y.O. (1982). Body temperature responses of Savanna Brown goat to the harmattan and hot-dry season. International Journal of Biometeorology, 26: 225 – 230.
29. Ayo, J.O., Oladele, S.B., Fayomi, A., Jumbo, S.D. and Hambolu, J.O. 1998. Body temperature, respiration and heart rate in the Red Sokoto goat during the harmattan season. Bulletin of Animal Production and Health in Africa., 46: 161 - 166.
30. Ramnath, V., Rekha, P.S. and Sujatha, K.S. 2008. Amelioration of heat stress induced disturbances of antioxidant defense system in chicken by Brahma Rasayana. Evid- Based Compl Alt Med 5: 77-84.
31. Sujatha, V, Korde, J.P., Rastogi, S.K., Maini, S., Ravikanth, K. and Rekhe D.S. 2010. Amelioration of heat stress induced disturbances of the antioxidantdefense system in broilers. J.Vet.Med.Anim.Health. 2(3): 18-28.
32. Belge, F., Ciner, A. and Selcuk, M. 2003. Effect of stress produced by adrenocorticotropin on lipid peroxidation and some antioxidants in vitamin C treated and non-treated chickens. South African Journal of Animal Science, 33(3): 201 – 205.
33. Surai, P.F., Noble, R.C., Sparks, N.H.C. and Speake, R.K. 2000. Effect of long-term supplementation with arachidonic of docosahexaenoic acids on sperm production in the broiler chicken. Journal of Reproduction and Fertility 120(2): 257 - 264.
34. Surai, P.F., Fujihara, N., Speake, B.K., Brillard, J.P., Wishart, G.J. and Sparks, N.H.C. 2001. Polyunsaturated fatty acids lipid peroxidation and antioxidants protection in avian semen. Review. Asian-Australasian Journal of Animals Science, 14(7): 1024 -1050.
35. Agarwal, A., Nallela, K.P., Allamaneni, S.S.R. and Said, T.M. 2004. Role of antioxidants in treatment of male infertility: An overview of the literature. Reproductive Biomedicine Online, 8(6): 616 - 627.
36. Agarwal, A., Prabhakaran, S.A. and Sikka, S.C. 2007. Clinical relevance of oxidative stress in patients with male factor infertility: Evidence-based analysis. American Urological Association Update, 26: 1-2.
37. Surai, P.F. 2002. Selenium in poultry nutrition 1. Antioxidants properties, deficiency and toxicity. World Poultry Science Journal, 58(3): 333 - 347.
38. Surai, P.F. 2010. Natural antioxidants in poultry nutrition: New developments. In Proceedings of the 16th European Symposium on Poultry Nutrition pp 669 – 675.
39. Aydemir, T., Ozturk, R., Bozkaya, L.A. and Tarhan, L. 2000. ‘Effects of antioxidant vitamins A, C, E and trace elements Cu, Se on CuZn SOD, GSH-Px, CAT and LPO levels in chicken erythrocytes’, Cell Biochemistry and Function 18(2), 109–115.
40. Balogun, T.F., Afolayan, S.B., Njoku, P.C. and Dafwang, I.I. 1996. Effect of ascorbic acid (vitamin C) and acetysalycilic acid (aspirin) supplementation of broiler diets on performance during the hot season. Samaru Journal of Agriculture Education, 5: 80 – 86.
41. Tuleun, C.D., Njoku, P.C. and Okwori, A.I. 2010. Effects of dietary ascorbic acid on performance of broiler chickens exposed to chickens exposed to different lighting regime. International Journal of Poultry Science. 9(2): 118 – 125.
42. Srikandakumar, A., Johnson, E.H. and Maghoub, O. 2003. Effect of heat stress on respiratory rate, rectal temperature and blood chemistry in Omani and Australian Merino sheep. Small Rum. Res.49: 193- 198.
43. Elsayed, N.M. and Bendich, A. 2001. Dietary antioxidants: Potential effects on oxidative products in cigarette-smoke. Nutrition Research, 21: 551 – 567.
44. Jadhav, N.V., Awati, B., Kulkarni, S., Waghmare, P.G., Suranagi, M.D., Ravikanth, K., Dandale, M. and Maini, S. 2014. Heat stress amelioration and production performance in layers supplemented with herbal liquid anti-stressor product. Journal of Veterinary Medicine and Animal Health, 63(3): 69 - 74.
45. Candan, F., Gultekin, F. and Candan, F. 2002. Effect of vitamin C and zinc on fragility and lipid peroxidation in zinc-deficient haemodialysis patient. Cell Biochemistry and Function, 20: 95 - 98.
46. Ajakaiye, J.J., Perez-Bello, A. and MollinedaTrujillo, A. 2010. Impact of vitamin C and E dietary supplementation on leukocyte profile of layer hens exposed to high ambient temperarure and humidity. Act Vet Brno, 79: 377 – 383.
47. Altan, O., Altan, A., Cabuk, M., Bayraktar, H. 2000. Effects of heat stress on some blood parameters in broilers. Turk J Vet Anim Sci, 24: 145-148.
48. Huff, G.R., Huff, W.E., Balog, J.M., Rath, N.C., Anthony, N.B. and Nestor, K.E. 2005. Stress response differences and disease susceptibility refected by heterophil to lymphocyte ratio in Turkeys selected for increased body weight. Poult Sci 84: 709-717.
49. Altan, O., Pabuccuaoglu, A., Konyalioglu, S. and Bayracktar, H. 2003. Effect of heat stress on oxidative stress, lipid peroxidation and some stress parameters in broilers. British Poultry Science, 44 (4): 54 - 55.
50. Langsdorf, L.J. and Zydney, A.L. 1993. Effects of uremia on the membrane transport characteristics of red blood cells. Blood, 81: 820- 822.