Effect of NaCl stress on chlorophyll biosynthesis, proline, lipid peroxidation and antioxidative enzymes in leaves of salt-tolerant and salt-sensitive barley cultivars

Bu araştırmada, tuza toleranslı (Avcı-2002) ve tuza hassas (Tokak 157/37) arpa çeşitlerinin tuza toleransları analiz edilmiştir. Fideler 0, 100, 200 ve 300 mM NaCl içeren hidroponik kültür ortamında büyütülmüştür. Kuru ağırlıktaki azalma Avcı-2002’ye göre Tokak 157/37 çeşidinde daha önemli bulunmuştur. Diğer taraftan, δ aminolevülinik asit dehidrataz - (ALAD) aktivitesi ve klorofil içeriği arasında önemli korelasyon bulunmamıştır. Avcı-2002 çeşidinin yapraklarında klorofil ve karotenoid içeriği yalnızca 300 mM NaCl’de önemli düzeyde azalırken, Tokak 157/37 çeşidinin pigment içeriği tüm NaCl konsantrasyonlarında azalmıştır. 300 mM NaCl konsantrasyonunda, prolin içeriğindeki artış Tokak 157/37 çeşidine göre Avcı-2002 çeşidinde önemli düzeyde daha yüksek bulunmuştur. Malondialdehit (MDA) içeriği ile belirlenmiş lipit peroksidasyon seviyesi Avcı-2002’ye göre Tokak 157/37 çeşidinde daha yüksek bulunmuştur. Tuz stresi süperoksit dismutaz (SOD), guaiakol peroksidaz (POD), askorbat peroksidaz (APX) ve katalaz (CAT) aktivitesinde önemli artışa neden olmuştur. Bununla birlikte, Avcı-2002 çeşidinin Tokak 157/37 çeşidinde göre nispeten daha düşük SOD ve CAT aktivitelerine ve daha yüksek POD ve APX aktivitelerine sahip olduğu belirlenmiştir. Sonuç olarak, kuru ağırlık, pigment içeriği, prolin içeriği, lipit peroksidasyonu ile POD ve APX aktivite sonuçları nispeten tuza toleranslı Avcı-2002 ile iyi bir korelasyon göstermiştir.

Tuza-toleranslı ve tuza-hassas arpa çeşitlerinin yapraklarında klorofil biyosentezi, prolin, lipit peroksidasyonu ve antioksidant enzimler üzerine NaCl stresinin etkisi

In this research, the salt tolerance of salt-tolerant (Avcı-2002) and salt-sensitive (Tokak 157/37) barley cultivars were analyzed. The seedlings were grown in a hydroponic culture containing 0, 100, 200 and 300 mM NaCl. The decrease in dry weight was more significant in Tokak 157/37 than in Avcı-2002. On the other hand, no significant correlations were found between the altered δ-aminolevulinic acid dehydratase (ALAD) activity and the chlorophyll content. The chlorophyll and carotenoid contents in leaves of Avcı-2002 decreased significantly at only 300 mM NaCl, whereas the pigment contents of Tokak 157/37 decreased at all NaCl concentrations. The increase in proline content was significantly higher in Avcı-2002 than in Tokak 157/37 at 300 mM NaCl. The lipid peroxidation level measured in terms of malondialdehyde (MDA) content was higher in Tokak 157/37 than in Avcı-2002. Salt stress caused significant increases in superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) activity. However, Avcı-2002 had relatively lower SOD and CAT activities and higher POD and APX activities than Tokak 157/37. As a result, dry weight, pigments content, proline content, lipid peroxidation and activities of POD and APX results are in good correlation with supporting Avcı-2002 being relatively salt-tolerant.

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