Faisal MEHDI, Kazim ALI, (Nesheman HUMA, Iqbal HUSSAIN, Abid AZHAR, Saddia GALANI

Comparative Biochemical Analysis of High and Low Sucrose Accumulating Sugarcane Varieties at Formative Stage under Heat Stress

Sugarcane (Saccharum officinarum L) is a valuable cash cropwhich plays an imperative role in the worldwide economy.However, high temperature has significantly retarded thecrop growth and yield by alteration of biochemical pathways.Therefore, the biochemical activities of two sugarcanevarieties were explored under heat stress condition. Thesugarcane cultivars S2003-US-633 (high sucroseaccumulation) and SPF-238 (low sucrose accumulation)were cultivated and subjected to different temperatureregimes i.e. control at 30±2 °C, heat stress at 45±2 °C andrecovery at 30±2 °C for 24, 48 and 72 hours at formativestage. Detailed profiling of physiochemical attributes, sugaranalysis linked with sucrose metabolism enzymes andthermotolerance indicators were investigated. S2003-US633 exhibited better response in terms of sugar accumulationregulated by sucrose synthase, sucrose phosphate synthaseand invertase activities along with more prolineaccumulation, total soluble protein contents with response tohigh temperature exposure. While S2003-US-633 is rankedas tolerant variety due to less MDA, H2O2 content andelectrolytes leakage exhibiting its efficient tolerancemechanism, giving high sugar recovery rate despite harshenvironmental conditions. Thus, these findings can behelpful in providing information for engineering sugarimprovement along with thermotolerance in sugarcanevarieties and providing new avenues towards the economicdevelopment of the country.

PDF

___

Abrahám E, Hourton-Cabassa C, Erdei L & Szabados L (2010). Methods for determination of proline in plants. In Plant Stress Tolerance pp. 317-331
Akram N A, Ashraf M & Al-Qurainy F (2012). Aminolevulinic acid-induced changes in some key physiological attributes and activities of antioxidant enzymes in sunflower (Helianthus annuus L.) plants under saline regimes. Scientia Horticulturae 142: 143-148
Albacete A, Grosskinsky D K & Roitsch T (2011). Trick and treat: a review on the function and regulation of plant invertases in the abiotic stress response. Phyton 50(2): 181-204
Ansari M I, Yadav A & Lal R (2013). An-overview on invertase in sugarcane. Bioinformation 9(9): 464
Apel K & Hirt H (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annual Review of Plant Biology 55: 373-399
Bates L S, Waldren R P & Teare I D (1973). Rapid determination of free proline for water-stress studies. Plant and Soil 39(1): 205-207
Bradford M M (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1-2): 248-254
Boaretto L F, Carvalho G, Borgo L, Creste S, Landell M G, Mazzafera P & Azevedo R A (2014). Water stress reveals differential antioxidant responses of tolerant and non-tolerant sugarcane genotypes. Plant Physiology and Biochemistry 74: 165-175
Botha F C & Black K G (2000). Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Functional Plant Biology 27(1): 81-85
Christensen J H & Christensen O B (2007). A summary of the prudence model projections of changes in European climate by the end of this century. Climatic Change 81(1): 7-30
Ebrahim M K, Zingsheim O, El-Shourbagy M N, Moore P H & Komor E (1998). Growth and sugar storage in sugarcane grown at temperatures below and above optimum. Journal of Plant Physiology 153(5-6): 593-602
Goel A & Sheoran I S (2003). Lipid peroxidation and peroxide-scavenging enzymes in cotton seeds under natural ageing. Biologia Plantarum 46(3): 429-434
Hare P D, Cress W A & Van Staden J (1998). Dissecting the roles of osmolyte accumulation during stress. Plant, Cell & Environment 21(6): 535-553
Heath R L & Packer L (1968). Photo peroxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125(1): 189-198
Hedge J E & Hofreiter B T (1962). Estimation of starch by anthrone reagent. Methods in Carbohydrate Chemistry pp. 9-10
Hubbard N L, Huber S C & Pharr D M (1989). Sucrose phosphate synthase and acid invertase as determinants of sucrose concentration in developing muskmelon (Cucumis melo L.) fruits. Plant Physiology 91(4): 1527-1534
Joshi B J, Krishnaveni S, Vijayalakshmi D, Sudhagar R & Raveendran M (2013). Activities of enzymes involved in synthesis and degradation of sucrose in popular sugarcane varieties. Asian Journal of Experimental Biology 4(2): 237-244
Kaur S, Singh H P, Mittal S, Batish D R & Kohli R K (2010). Phytotoxic effects of volatile oil from Artemisia scoparia against weeds and its possible use as a bioherbicide. Industrial Crops and Products 32(1): 54-61
Laemmli U K (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227(5259): 680
Liu X, Huang B (2000). Heat stress injury in relation to membrane lipid peroxidation in creeping bentgrass. Crop Science 40: 503-513
Loreto F & Velikova V (2001). Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiology 127(4): 1781-1787
McCormick A J, Watt D A & Cramer M D (2008). Supply and demand: sink regulation of sugar accumulation in sugarcane. Journal of Experimental Botany 60(2): 357-364
Miller G L (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry 31(3): 426-428
NASA (2017). NASA’ s Goddard Institute for Space studies (GISS). https://climate.nasa.gov/vital-signs/globaltemperature/ (July 2017)
PSMA (2007) (Pakistan sugar mill association). Annual Report pp. 4
Potters G, Pasternak T P, Guisez Y, Palme K J & Jansen M A (2007). Stress-induced morphogenic responses: growing out of trouble? Trends in Plant Science 12(3): 98-105
Roitsch T & González M C (2004). Function and regulation of plant invertases: sweet sensations. Trends in Plant Science 9(12): 606-613
Ruan Y L, Jin Y, Yang Y J, Li G J & Boyer J S (2010). Sugar input, metabolism, and signaling mediated by invertase: roles in development, yield potential, and response to drought and heat. Molecular Plant 3(6): 942-955
Savchenko G E, Klyuchareva E A, Abramchik L M & Serdyuchenko E V (2002). Effect of periodic heat shock on the inner membrane system of etioplasts. Russian Journal of Plant Physiology 49(3): 349-359
Shrivastava P K, Kaleriya K A, Parmar V & Patil R G (2010). Time Series Analysis of Temperature Variations and its Impact on Sugarcane Crop. In National Symposium on Climate Change and Rainfed Agriculture, Organized by Central Research Institute for Dryland Agriculture at Hyderabad February 18-20
Tana B, Chanprame S, Tienseree N & Tadakittisarn S (2014). Relationship between invertase enzyme activities and sucrose accumulation in sugarcane (Saccharum spp.). Kasetsart Journal: Natural Science 48: 869-879
Vorster D J & Botha F C (1999). Sugarcane internodal invertases and tissue maturity. Journal of Plant Physiology 155: 470-477
Yang G, Rhodes D & Joly R J (1996). Effects of high temperature on membrane stability and chlorophyll fluorescence in glycinebetaine-deficient and glycinebetaine-containing maize lines. Functional Plant Biology 23(4): 437-443
Zhang J H, Huang W D, Liu Y P & Pan Q H (2005). Effects of temperature acclimation pretreatment on the ultrastructure of mesophyll cells in young grape plants (Vitis vinifera L. cv. Jingxiu) under cross‐temperature stresses. Journal of Integrative Plant Biology 47(8): 959-970