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Ayçiçeği (Helianthus annuus L.)' nin olgunlaşmamış embriyo ve kotiledon ekspalntlarından adventif sürgün rejenerasyonu

Ayçiçeğinin Ekiz genotipinden seçilen 10 hattın olgunlaşmamış embriyo ve kotiledon eksplanları 20 mg/l sukroz ve farklı oranlarda kinetin ve naftalen asetik asit (NAA) içeren Murashige and Skoog (MS) ortamında kültüre alınmıştır. Denemelerde 1. hafta sonunda rejenerasyonu olumsuz etkileyen kaçak kökler steril koşullarda eksplantlardan uzaklaştırılmıştır. Özellikle olgunlaşmamış embriyo eksplantında çalışılan tüm hatlarda ve ortamlarda kallus ve sürgün oluşumu görülürken olgunlaşmamış kotiledon eksplantında tüm ortamlarda iyi bir kallus oluşumu gerçekleşmiş ancak daha az sürgün elde edilmiştir. Kallus çapı ve ağırlığının sürgün gelişimine etkisi olmadığı görülmüştür. Bitki doku kültürü çalışmalarında kullanılan eksplantların sürgün gelişimi için önemli faktörlerden birisi olduğu gözlenmistir.

Adventitious shoot regeneration from immature embryo and cotyledon explants of sunflower (Helianthus annuus L.)

Abstract: Immature embryo and cotyledons of 10 lines of sunflower (Helianthus annuus L) were cultured on Murashige and Skoog (MS) medium supplemented with 20 mg/l sucrose and different concentrations of Kinetin and α Naphthalene acetic acid (NAA). Development of precocious roots was observed after one week of culture, which were removed aseptically to induce callus and shoot regeneration. Induction of healthy shoots was observed on cotyledon and embryo explants on MS medium containing any concentration of kinetin and and NAA. This is an important development in the tissue culture research of sunflower.
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