There are many olive (O/ea europaea L.) varieties grown in Anatolia, vvhich have not been characterized (morphologic and biologic characteristics), yet. Some of them that have been identified by morphologic and biological characteristics, but stili have discriminate some variations due to unknovvn genotypic or phenotypic causes. Genetic diversity studies using Random Amplified Polymorphic DNA (RAPD) technique were carried out in a set of 10 olive cultivars (Ayvalik, Derik Halhali, Domat, Gemlik, Kilis Yaglik, Manzanilla, Memecik, Nizip Yaglik, Sari Ulak and Tavsan ,Yüreği) trom the Turkish Olive Germplasm Bank of the Olive Research Institute in İzmir, Turkey. Analysis of PCR Products was achieved by using the simple matching coeffîcient and UPGMA clustering. Primers were identified and used in combination to discriminate betvveen different varieties. The diversity of RAPD patterns demonstrated the biodiversity among varieties. Also some pomological and biochemical analysis vvere done in order to support the results of the genetic variability. Among cultivars, Derik Halhali shovved the big differences, according to the genetic and biochemical results.