Recep SÜTÇÜ, Duygu DOĞUÇ, Onur AKTÜRK, İrfan ALTUNTAŞ, Namık DELİBAŞ

Subkronik nikotin uygulamasının ratlarda lipid peroksidasyonu ve antioksidan enzim aktivitelerine etkisi

Sigara içimi modern ülkelerde önlenebilir ölüm nedenlerinden biridir. Nikotin sigara dumanının major toksik bileşeni olup, sigara içenlerde kardiyovasküler hastalıklar ve akciğer kanserinin gelişiminde önemli bir rol oynar. Bu çalışmada genç ve yaşlı subkronik nikotin uygulamasının genç ve yaşlı ratlarda antioksidan enzim aktiviteleri ve lipid peroksidasyonu üzerine olan etkilerini araştırmayı planladık. Bu amaçla genç nikotin, genç kontrol, yaşlı nikotin, yaşlı kontrol grubu olmak üzere 4 grup oluşturduk. Her grup 10 erkek Sprague Dawley rattan oluşuyordu. Nikotin ratlara 0.45 mg/kg olacak şekilde, günde iki kez 18 gün boyunca cilt altına enjekte edildi. Kontrol grubuna da eş zamanlı olarak serum fizyolojik enjeksiyonu yapıldı. Deney sonunda kan örnekleri alınarak antioksidan enzim aktiviteleri ve malondialdehit düzeyleri ölçüldü. Subkronik nikotin uygulamasının antioksidan enzim aktivitelerinde ve malondialdehit düzeylerinde önemli bir değişikliğe yol açmadığı görüldü.

Anahtar Kelimeler:

Nikotin, Malondialdehit, Lipid peroksidasyonu, Antioksidanlar, Sıçan, Sprague-Dawley, Oksidatif stres

The effects of subchronic nicotine administration on level of the lipid peroxidation and antioxidant enzymes activities in rats.

Smoking is one of the causes of death which can be prevented in modern countries. Nicotine, a major toxic component of cigarette smoke, plays an important role in the development of cardiovascular disease and lung cancer in smokers. In this study we have undertaken to examine the effects of subchronic nicotine administration on the antioxidant enzyme activities and malondialdehyde levels in young and old rats. For this purpose we arranged 4 groups as; young nicotine, aged nicotine, young control and aged control. Each group includes 10 male Sprague dawley rats. Rats were injected subcutaneously with 0.45 mg/kg daily nicotine for 18 days. Control groups were injected normal saline at same time. At the end of experiment, malondialdehyde and antioxidant enzyme activities were assayed in erythrocyte samples. It was observed that subchronic nicotine administration was not lead to a significant change.

Keywords:

Nicotine, Malondialdehyde, Lipid Peroxidation, Antioxidants, Rats, Sprague-Dawley, Oxidative Stress,

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