Meryem AKPOLAT, Bayram KAMAT, Kanat GÜLLE, Bekir Hakan BAKKAL

Skrotal Radyoterapide Curcuminin Profilaktik Kullanımı Testis Dokusunda PARP-1 İmmünreaktivitesini ve Spermatogenezi Nasıl Etkiler ?

Fertilitede akut doz radyasyonun öldürücü etkileri iyi bilinmektedir. Kısa süreliradyasyona maruziyet germ hücrelerinin ölümü ve hasarlanması neticesindeinfertilite ile sonuçlanır. Son yıllarda radyoterapi, kanserli hastaların uzunsüreli iyileşmesine önemli ölçüde katkı vermiştir. Ancak radyasyonun uzundönemde üreme sistemi üzerindeki hasarlayıcı etkisi ile meydana gelen fertilizasyonkayıplarını göz ardı etmemek gerekmektedir. Bu güne kadar curcumininantioksidan ve radyoprotektif etkisi ile ilgili birçok makale yayınlanmıştır.Bu çalışma; curcuminin X ışınlamayı takiben sıçan testisinde ortayaçıkacak germ hücre kaybı ile ilişkili infertiliteyi iyileştirebileceği düşüncesi ileplanlanmıştır. Bu deneysel çalışmada biri kontrol 3’ü deney grubu olmak üzeretoplam 4 grup oluşturuldu. Radyasyon hasarı oluşturmak amacıyla kontrolve curcumin grubu dışındaki deneklerin skrotal bölgelerine tek seferde 6 GyX ışını uygulandı. Curcumin ve radyasyon+ curcumin gruplarına; ışınlamadan1 hafta önce başlayarak, haftada 3 kez olmak üzere 7 hafta boyunca oralyoldan 100 mg/kg curcumin, kontrol ve radyasyon gruplarına da aynı şekildeçeşme suyu verildi. Işınlamadan 6 hafta sonra tüm denekler sakrifiye edildi vealınan testis biyopsi materyalleri histopatolojik incelemeler için işlemlendirildi.Bu çalışmada iyonize radyasyonun tübüllerin çoğunda germ hücre kaybınabağlı olarak, tübüler atrofiye neden olduğu görüldü. Radyasyon alan deneklerintümünde, spermatogenezisin bozulması ile ilişkili olarak, Jhonsen skorundakontrole göre anlamlı düzeyde azalma saptandı. PARP-1 immünreaktiviteskorunda da hasarının şiddeti ile ilişkili olarak, kontrole göre anlamlı düzeydeartış belirlendi. Curcumin uygulamasının ise radyasyona bağlı ortaya çıkanhasarları önlemede beklenen düzeyde koruma ve iyileşme sağlamadığı tespitedildi.

How the Profilactic Usage of Curcumin Affects the Parp-1 Immunoreactivity And Spermatogenesis in Scrotal Radiotherapy?

The lethal effects of acute doses of radiation on fertility are well known. Germ cells are killed or damaged within a short time of exposure to radiation. In recent years, radiotherapy of patients with cancer has led to an increased number of sustained remissions. However, among the long-term side effects of radiation, injury to the reproductive system is of particular concern. Many papers have been reported so far on the antioxidant effect of curcumin. The present study, we hypothesized that curcumin can minimize germ-cell deple- tion and morphological features of late cell damage in the rat testis following X-irradiation. The animals were divided into 4 groups: the first group was the control and the other three were the experimental groups. 6 Gy X ray in a single fraction was applied into the scrotal areas of the subjects except the control and curcumin groups in order to form radiation damage. The rats in the second and forth groups were given curcumin (a dose of 100 mg/kg body weight) orally three times a week during a seven-week period, beginning the week before radiation therapy. The rats in the first and third groups received tap water in the same way. Testis biopsy samples from the all groups were taken on the 7th week. All samples were processed and observed at the light microscopic levels. In the present study, radiation exposure caused severe degenerative changes in testes. Spermatogenesis had arrested in seminiferous tubules and the majority of the tubules were found to be atrophic, absent of germ cells. It was identified that curcumin hadn’t been effective in the prevention of all damages caused by radiation.

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