Aslı ÖZKIZILCIK KOÇYİĞİT, İbrahim PİRİM, Tülay KILIÇASLAN AYNA, Mustafa SOYÖZ

Detection of Anti-HLA Antibodies Produced After Transplantation in Renal Transplants and Evaluation of Its Association with the Other Parameters

Object: The purpose of the study was to evaluate the immunological responseto graft and its association with related factors in the sera samples collectedperiodically from patients transplanted from deceased or alive donorsin Izmir Tepecik Education and Research Hospital. Material and Method:Anti-HLA antibody screening and identification tests of 31 patient sera sampleswere tested by flow cytometric method. The sera samples were collectedperiodically (1st day, 1st week, 2nd week, 4th week, 12th week, 24th weekand 52nd week) after transplantations in our hospital. After the screening of all samples, anti-HLA antibodies of PRA positivesamples were identified. All of procedures were performedaccording to the manufacturer’s instructions.The other parameters were statistically evaluated byPearson correlation test. Results: Of the patients,12.9% and 6.45% were only class I and only classII positive in the post-transplant 52nd week, respectively.The patients were not positive for class I and IIantibodies at the same time. It was observed that thepatients did not produce donor specific antibodies onthe 1st day, 1st, 2nd, and 4th week after transplantation.The antibodies were produced after the 12th weekin a patient. There were statistically significant correlationsbetween last creatinine levels-donor ages andGFR values-patient ages (p

Böbrek Nakli Olan Hastalarda Nakil Sonrasında Oluşan Anti-HLA Antikorlarının Saptanması ve Çeşitli Parametrelerle İlişkisinin Değerlendirilmesi

Amaç: Bu çalışmada İzmir Tepecik Eğitim ve Araştırma Hastanesi’nde kadavra veya canlı donörden böbrek nakli olmuş hastaların nakil sonrasında periyodik olarak toplanan serum örneklerinde grefte karşı gelişen immünolojik cevabın ve ilgili faktörlerle arasındaki ilişkisinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntem: 31 hasta serumunun anti-HLA antikor tarama ve tanımlama testleri flow sitometrik yöntemle yapılmıştır. Bu serum örnekleri, hastanemizde nakilden sonra periyodik olarak (1. gün, 1. hafta, 2. hafta, 4. hafta, 12. hafta, 24. hafta ve 52. hafta) toplanmıştır. Bütün örneklere tarama testi yapılırken, sadece PRA pozitif olan serum örneklerine anti-HLA antikor tipini belirlemek amacıyla tanımlama testi uygulanmıştır. Bütün prosedürler üretici firmanın talimatlarına göre yapılmıştır. Diğer parametreler Pearson korelasyon testiyle istatistiksel olarak değerlendirilmiştir. Bulgular: Nakilden sonra 52. haftadan sonra hastaların sırasıyla %12,9 ile %6,45’i anti-HLA sınıf I ve II antikorları bakımından pozitif bulunmuştur. Hastalarda 1. gün, 1., 2., ve 4. haftalarda mismatch antijenlere karşı antikor oluşumu gözlenmemiştir. Bir hastada antikorlar 12. haftadan itibaren oluşmuştur. Yapılan korelasyon analizlerinde hastaların nakil sonrası son kreatinin değerleriyle donör yaşları ve GFR değerleriyle hasta yaşları arasında istatistiksel olarak anlamlı sonuçlar elde edilmiştir (sırasıyla p

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