In Vitro Cytotoxic Evaluation Of Conventional Denture Base Material And Soft Lining Material Using Colorimetric Mtt Assay

Purpose: In this study, it was aimed to evaluatethe time course of cytotoxic effects of theconventional base material and soft lining materialon the mouse fibroblast cells.Material andMethod: Disc-shaped test samples of denturebase material (Rodex) and soft lining material(Dentusil) were fabricated according to manufacturers'instructions under aseptic conditions.The samples were subjected to 5,000 thermalcycling to mimic the oral environment. Followingaging procedures, the cytotoxic effect ofthe materials was assessed by [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2Htetrazoliumbromide] assay using L929 mousefibroblast cells after 24 h, 48 h and 72 h cellincubation period. Cell viability values werecalculated for each group. Statistical analysis ofthe data was performed using a two-way repeatedmeasurement method. (p

Kolorimetrik Mtt Testi Kullanarak Geleneksel Protez Kaide Materyali İle Yumuşak Astar Materyalinin İn Vitro Sitotoksik Özelliklerinin Değerlendirilmesi

Amaç: Bu çalışmada protez yapımında kullanılan geleneksel kaide materyali ile yumuşak astar materyalinin fare fibroblast hücreleri üzerinde zamanla meydana gelen sitotoksik etkilerinin değerlendirilmesi amaçlandı. Materyal ve Metod: Protez kaide materyali (Rodeks) ve yumuşak astar materyalinin (Dentusil) disk şekilli test numuneleri üreticinin talimatlarına göre aseptik şartlar altında hazırlandı.. Örnekler, ağız ortamını taklit etmek için 5.000 termal döngüye tabi tutuldu. Yaşlandırma prosedürlerini takiben, materyallerin sitotoksik etkisi, 24 saat, 48 saat ve 72 saatlik hücre inkubasyon döneminden sonra L929 fare fibroblast hücreleri kullanılarak [3-(4,5-dimethylthiazol-2- yl)-2,5-diphenyl-2H-tetrazolium bromide] testi ile değerlendirildi. Her grup için hücre canlılığı değerleri hesaplandı. Verilerin istatistiksel analizi, iki yönlü tekrarlanan bir ölçüm yöntemi kullanılarak gerçekleştirildi. (P

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Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi-Cover
  • ISSN: 1300-7416
  • Yayın Aralığı: 4
  • Başlangıç: 1994
  • Yayıncı: SDÜ Basımevi / Isparta
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