GUNDELİA TOURNEFORTİİ L. BİTKİ EKSTRAKTLARININ ANTİMİKROBİYAL AKTİVİTELERİNİN VE İNVİTRO SİTOTOKSİK AKTİVİTELERİNİN DEĞERLENDİRİLMESİ

Giriş Kenger, Asteraceae familyasından Gundelia tournefortii bitkisi, tıbbi bir bitkidir. Kenger’in çiçeklerinin, yapraklarının, tohumlarının ve köklerinin besin kaynağı olarak kullanıldığı bilinmektedir.Gereç ve Yöntem Bu çalışmada Sivas ilinde, taze filizleri soyularak çiğ ve pişirilerek yenilen Kenger’in gövde kısımlarının su fazı ve uçucu yağ ekstraksiyonu yapılarak, özütlerin antimikrobiyal ve in vitro antiproliferatif özellikleri bakımından değerlendirilmesi amaçlanmıştır. Sitotoksik aktiviteleri Meme Kanseri Hücre Hattı (MCF-7) ve insan endotelyal hücre hattı (HUVEC) kullanılarak araştırılmıştır. Bitki özütlerinin hücre kültürlerindeki etkileri, XTT yöntemiyle Eliza reader cihazında absorbans bulunarak elde edilmiştir. Kenger bitki özütlerinin MCF-7 hücreleri ve endotelyal hücreler üzerinde sitotoksik etkileri belirlenmiştir.Bulgular Kenger bitki özütlerinin zayıf antimikrobiyal etkilerinin olduğu MIC değerleriyle ortaya konulmuştur.Sonuç Sivas ilinde halk arasında şifalı olduğu bilinen, bahar ve yaz aylarında büyüyen bitki kısımları kesilip soyularak iç kısımlarının besin olarak tüketildiği, çeşitli hastalıkların tedavisinde kullanılan Kenger bitkisinin, denediğimiz hücreler üzerinde sitotoksik etkilerinin olduğu, zayıf antimikrobiyal etkisinin bulunduğu görülmektedir.

EVALUATION OF ANTIMICROBIAL ACTIVITIES AND INVITRO CYTOTOXIC ACTIVITIES OF GUNDELIA TOURNEFORTII L. PLANT EXTRACTS

ObjectiveKenger, Gundelia tournefortii plant from Asteraceae family, is a medicinal plant. Flowers, leaves, seeds and roots of Kenger have been reported to be usedas a source of food.Material and MethodsIn this study, it was aimed to evaluate the antimicrobial and in vitro antiproliferative properties of Kenger which fresh sprouts are peeled and eated cooked or uncooked in Sivas. The water phase and essential oil extraction of the body parts of Kenger were aimed to be evaluated in terms of their antimicrobial and in vitroantiproliferative properties.ResultsCytotoxic activities were investigated using the Breast Cancer Cell Line (MCF-7) and human endothelial cell line (HUVEC). The effects of plant extracts on cellcultures were determined by the absorbance of Eliza reader by XTT method. Kenger plant extracts have been shown to have weak antimicrobial effects withMIC values. ConclusionsIn the province of Sivas, growing parts of the plant in the spring and summer are cut and peeled into the inner parts of the nutrients. Kenger, which is used in thetreatment of various diseases, has cytotoxic effects on the cells we tested and has a weak antimicrobial effect.

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