Osman GÖKALP, İnanç KARAKOYUN, SÜLEYMAN KALELİ, Mehmet Kaya ÖZER, FATİH GÜLTEKİN

Chlorpyrifos ethyl'in rat pankreası üzerine etkisi

Pankreas bazı ksenobiotiklere göreceli olarak daha hassas bir organdır. Organofosfat zehirlenmelerinde ortalama % 12 oranında akut pankreatite rastlandığı bildirilmiştir. Bu çalışmada organofosfat insektisitlerden chlorpyrifos ethyl (CE)'in tolere edilebilir maksimum dozda pankreas üzerine olası etkilerini genç ve erişkin vatlarda araştırmayı planladık. Çalışma grupları; genç kontrol grubu, genç CE verilen grup ve erişkin CE verilen grup şeklinde düzenlendi. Bu gruplarda üçer alt gruba ayrılarak deney başladıktan sonra 2, 7 ve 14 gün sonra kan örnekleri alındı. Her grubun deney süresinin bittiği günde kan örnekleri alınarak plazmada kolinesteraz (ChE), amilaz ve lipaz aktiviteleri ile malondialdehit (MDA) düzeyleri ölçüldü. Kontrol grubuyla karşılaştırıldığında CE uygulanan grubun ChE aktivitesinin anlamlı olarak azaldığı görüldü. CE uygulanan grup ile kontrol grubu karşılaştırıldığında amilaz aktivitesinin ve MDA düzeyinin anlamlı olarak arttığı görüldü. Sonuç olarak bu bulgulardan CE'nin maksimum tolare edilebilir dozda ratlara uygulandığında hafif pankreas hasarına neden olabileceği fakat bunun oksidatif stres artışı veya kolinesteraz enzim aktivitesin inhibisyonu ile doğrudan yakın ilişki halinde olmadığı düşünüldü.

Anahtar Kelimeler:

Erkek, Kolinesterazlar, Modeller, hayvan, Pankreas, Organotiyofosfor bileşikleri, Malondialdehit, Klorpirifos, Amilazlar, Sıçan, Sprague-Dawley, Pankreatit

The effects of chlorpyrifos-ethyl on pancreas in rats

Pancrease is an organ which realtively vulnareble to various xenobiotics. It is reported that organophospahates cause acute pancreatitis at approximately 12% rate. We aimed to investigate effects of chlorpyrifos-ethyl (CE) [O,O-diethyl-O-(3,5,6-trichloro-2-pyridyl)phosphorothioate], an organophosphate insecticide, on pancrease at the maximum tolerable dose within various duration. Study groups designed as control group, CE treated (administered single tolerable dose) adult group and CE treated (administered single tolerable dose) infantil group. Each group divided into three subgroups. Animals were sacrificied at days 2., 7. and 14. We examined effects of CE on serum activities of cholinesterase (ChE), amylase and lipase enzymes in plasma and, blood levels of malondialdehyde (MDA) in erythrocytes. Compared to control group, ChE activity was found to be significantly decreased while amylase activity and MDA level were found to be sigificantly elevated in CE treated group. Compared to CE treated group, lipase activity was found to be increased in CE treated group. Based on the findings that were obtained from this study we can conclude that maximum tolerable dose of CE may cause pancreas disorder that is probably not directly related to increased oxidative stress or inhibition of cholinesterase enzyme activity.

Keywords:

Male, Cholinesterases, Models, Animal, Pancreas, Organothiophosphorus Compounds, Malondialdehyde, Chlorpyrifos, Amylases, Rats, Sprague-Dawley, Pancreatitis,

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1. Hsiao CT, Yang CC, Deng JF, Bullard MJ, Liaw SJ. Acute pancreatitis following orgaphosphate intoxication. J Toxicol Clin Toxicol. 1996;34:343-47.
2. Sahin I, Onbasi K, Sahin H, Karakaya C, Ustun Y, Noyan T. The prevelance of pancreatitis in organophosphate poisonings. Hum Exp Toxicol. 2002;21:175-77.
3. Hegyi P, Takacs T, Tiszlavicz L, Czako L, Lonovics J. Recovery of exocrine pancreas six months following pancreatitis induction with L-arginine in streptozotocin- diabetic rats. J Physiol Paris. 2000;94(1):51-5.
4. A.J. Bone, D.J. Gwillam, Animal models of insulin- dependent diabetes mellitus. 1998, Chapter 16, pp. 16.1-16.
5. The Expert Committee on The Diagnosis and Classification of Diabetes Mellitus. Report of the expert committee on the diagnosis and classification of diabetes mellitus. Diab Car 1999;22 (Supplement 1): S5-S19.
6. Rizos E, Liboropoulos, Kosta P, Efremidis S, Elisaf S. Carbofuran-induced acute pancreatitis. JOP. J Pancreas 2004; 5(1):44-7.
7. Gökalp O, Mollaoğlu H, Yılmaz HR, Altuntaþ I. Fenthion'un pankreas üzerine etkileri: Vitamin E ve C'nin rolü. Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi 2003; 10(2): 21-3.
8. Mollaoğlu H, Yılmaz HR, Gökalp O, Altuntaş I. Methidathion'un pankreas üzerine etkileri: Vitamin E ve C'nin rolü. Van Týp Dergisi 2003; 10: 98-100.
9. Tinoco R, Halperin D. Poverty, production, and health: inhibition of erythrocyte cholinesterase via occupational exposure to organo-phosphate insecticides in Chiapas, Mexico. Arch Environ Health 1998; 53: 29-35.
10. Gokalp O, Buyukvanlı B, Cicek E, Ozer MK, Koyu A, Altuntas I, Koylu H. The effects of diazinon on pancreatic damage and ameliorating role of vitamin E and vitamin C. Pes Bio and Phy2005; 81(2): 123-8.
11. Demirin H, Büyükvanlı B, Öztürk Ö, Gökalp O, Yılmaz HR, Koyu A, Altuntaþ Ý, Köylü H. Karaciğerde organofosfat insektisit fosalonun yaptığı hasar: vitamin E ve C'nin etkileri. 30. Ulusal Fizyolojik Bilimler Kongresi. 31 Aðustos-3 Eylül 2004, Konya-TURKEY.
12. Dabrowski S, Hanke W, Polanska K, Makowiec- Dabrowska T, Sobala W. Pesticide exposure and birthweight: an epidemiological study in Central Poland. Int J Occup Med Environ Health 2003;16:31-9.
13. Lotti M. Promotion of organophosphate induced delayed polyneuropathy by certain esterase inhibitors. Toxicology 2002;27:181-182:245-248.Carr RL, Richardson JR, Guarisco JA, Kachroo A, Chambers JE, Couch TA, Durunna GC, and Meek EC. Effects of PBC exposure on the toxic impact of organophosphors insecticides. Toxicol Sci 2002;67:311-21.
14. Karalliadde L. Organophosphorus poisoning and anesthesia. Anesthesia 1999;54:1073-88.
15. Altuntaş I, Delibaş N. The effects of Fenthion on lipid peroxidation and some liver enzymes: The possible protective role Vitamins E and C. Turk J Med Sci. 2002;32:293-97.
16. Gültekin F, Delibaş N, Yaşar S, Kılınç I. In vivo changes in antioxidant systems and protective role of melatonin and a combination of vitamin C and vitamin E on oxidative damage in erythrocytes induced by chlorpyrifos-ethyl in rats. Arch Toxicol 2001;75:88-96.
17. Bagchi D, Bagchi M, Hassoun EA, Stohs SJ. In vitro and in vivo generation of reactive oxygen species. DNA damage and lactate dehydrogenase leakage by selected pesticides. Toxicol 1995;104:129-40.
18. Appenroth D, Frög S, Kersten L, Splinter FK. Protective effects of vitamin E and C on Cisplatin nephrotoxicity in developing rats. Arch Toxicol1997;71:677-83.
19. Dressel TD, Goodale RL Jr, Zweber B, Borner JW. The effect of atropine and duct decompression on the evolution of Diazinon-induced acute canine pancreatitis. Ann Surg. 1982 Apr;195(4):424-34.
20. Liu S, Oguchi Y, Borner JW, Runge W, Dressel TD, Goodale RL. Increased canine pancreatic acinar cell damage after organophosphate and acetylcholine or cholecystokinin. Pancreas 1990;5(2):177-82.
21. Panieri E, Krige JE, Bornman PC, Linton DM. Severe necrotizing pancreatitis caused by organophosphate poisoning. J Clin Gastroenterol. 1997;25:463-65.