Vitamin B Kompleksinin Fotodinamik Terapiyle Kolon Kanserine Etkisi

Amaç: Kolon kanserleri, hastanın yaşam kalitesini derinden etkileyen ve çeşitli komplikasyonlarla ilişkili kronik ölümcül bir hastalıktır. Vitamin B kompleksi, gıda takviyesi olarak alındığında olumlu anti-inflamatuar etkiler göstermektedir. Bu çalışma, fotodinamik terapiyle lipozomlu vitamin B kompleksinin kolon kanserlerinde (HCT-116 ve HT-29 hücre hattı) anti-kanser etkisini araştırmayı amaçlamıştır. Materyal- Metot: Çalışmanın birinci basamağını oluşturan Vitamin B kompleksi lipozom ile kapsüle edildi. Lipozomlu vitamin B kompleksi Zetasizer cihazı ile karakterize edildi. Lipozomlu vitamin B kompleksi MTT (3-4,5-dimetil-tiyazolil-2,5-difeniltetrazolyum bromür) testi ile HCT-116 ve HT-29 hücre hattında % canlılık oranlarına bakılmıştır. Aynı hücre hatlarında vitamin B kompleksinin fotodinamik terapi etkisiyle hangi ölüm yolaklarını seçtiğini göstermek için de akış sitometrisi kullanılarak analiz edilmiştir. Bulgular: Lipozomlu vitamin B kompleksinin boyutları 100 nm ile 200 nm aralığında değiştiği belirlendi. Lipozomlu vitamin B kompleksi (fotosensitizer) 7,5 J/cm2 ışık dozunda 0,25 mg/ mL'de karanlık deneyinde HCT-116 hücre canlılık yüzdesi %75 iken ışık deneyi sonrası bu oran %47’lere düşmüştür (****p<0.001). 0,5 mg/mL vitamin B kompleksi çözeltisi için yapılan karanlık deneyinde HT-29 hücre canlılık yüzdesi %70 iken ışık deneyi sonrası bu oran %35’lere düşmüştür (****p<0.001). Arzu edilen fotodinamik etkinlik bu sonuçlara daha yakındır. Hücre ölüm yolakları belirleme deneyinde lipozomlanmış vitamin B kompleksinin konsantrasyonu 1mg/mL olarak HCT-116 ve HT-29 hücrelerine uygulanmıştır. HCT-116 hücre hattında %40 oranında apoptoz görülürken HT-29 hücre hattında %60 oranında apoptoz görüldü. Sonuç: Pazarlanan ilaçları yeniden konumlandırmak, yeni antitümör ilaçları keşfetmek için yeni bir yaklaşım olarak ortaya çıkmıştır. Pazarlanan ilaçlar için yeni kullanım alanlarının geliştirilmesi, yeni anti-tümör ilaçları geliştirmenin etkili ve hızlı yolu olduğu kanıtlanmıştır. Verilerimiz in-vitro olarak bazı kolon kanser türlerinde vitamin B kompleksinin fotodinamik terapiyle kullanımının ümit vaat ettiğini savunmaktadır.

Anahtar Kelimeler:

Fotodinamik terapi, kolon kanseri, ilaç yeniden konumlandırma, vitamin B kompleksi, lazer

Effect of Vitamin B Complex on Colon Cancer by Photodynamic Therapy

Objective: Colon cancer is a chronic fatal disease that severely affects the quality of life of patients and is associated with various complications. Vitamin B complex shows positive anti-inflammatory effects when taken as a food supplement. The aim of this study was to investigate the anti-cancer effect of vitamin B complex with liposomes in colon cancers (HCT-116 and HT-29 cell lines) using photodynamic therapy. Material-Method: Vitamin B complex, which is the first step of the study, was encapsulated with liposome. Vitamin B complex with liposome was characterized by Zetasizer device. The MTT (3-4,5-dimethyl-thiazolyl-2,5-diphenyltetrazolium bromide) assay was used to determine the % survival rates in HCT-116 and HT-29 cell lines. The same cell lines were also analyzed by flow cytometry to show which death pathways were selected by the vitamin B complex with the effect of photodynamic therapy. Results: It was found that the dimensions of the vitamin B complex with liposomes varied between 100 nm and 200 nm. Liposomal vitamin B complex (photosensitizer) at 7.5 J/cm2 light dose at 0.25 mg/mL in the dark experiment, while the percentage of HCT-116 cell viability was 75%, this rate decreased to 47% after the light experiment (****p <0.001). While the percentage of HT-29 cell viability was 70% in the dark test for 0.5 mg/mL vitamin B complex solution, this rate decreased to 35% after the light test (****p<0.001). The desired photodynamic efficiency is closer to these results. In the cell death pathway assay, the concentration of liposomal vitamin B complex was applied to HCT-116 and HT-29 cells at 1 mg/mL. While 40% apoptosis was observed in the HCT-116 cell line, 60% apoptosis was observed in the HT-29 cell line. Conclusion: Repositioning of marketed drugs has emerged as a new approach to the discovery of new anti-tumor drugs. The development of new uses for marketed drugs has proven to be an efficient and rapid way to develop new anti-tumor drugs. Our data suggest that the in vitro use of vitamin B complex with photodynamic therapy is promising in some types of colorectal cancer.

Keywords:

Photodynamic therapy, colon cancer, drug repositioning, vitamin B complex, laser,

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