Ebru ÖNEM, Hasan Cumhur SARISU, Bashar İBRAHİM

Işkın (Rheum ribes L.) ekstraktlarının Bakteriyel İletişim Üzerine Etkisi ve Antibakteriyel Aktivitesi

Amaç: Bakteriyel dirençle mücadelede bakteriler arasındaki iletişimin engellenerek virülansın önüne geçilmesi, enfeksiyon hastalıklarıyla mücadelede yeni ve etkili bir seçenek olarak görülmektedir. Birçok bakteri gibi Pseudomonas aeruginosa da bu sistemi alkali proteaz, piyosiyanin, fosfolipaz ve endotoksin gibi virülans faktörlerini sentezlenmesinde, ayrıca enfeksiyon hastalıklarında önemli rol oynayan biyofilm oluşumu ve kayma hareketini yaparken kullanmaktadır. Materyal-Metot: Yapılan bu çalışmada ışkın (Rheum ribes L.) bitkisinin metanol (MeOH), metanol-kloroform (MeOH-CHCl3) ve su (H2O) ekstraktlarının kontrolü çevreyi algılama sistemi tarafından sağlanan P. aeruginosa PA01’in yapmış olduğu kayma hareketi üzerine inhibisyon etkisine bakılmıştır. Ayrıca Gram-pozitif Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 25923, Metisilin-Resistant Staphylococcus aureus ATCC 43300 ve Gram-negatif Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PA01 bakterileri üzerinde antibakteriyel etkileri araştırılarak minimum inhibisyon konsantrasyonları (MIK) belirlenmiştir. Bulgular: Antibakteriyel aktivite sonuçları, tüm ekstraktların çalışmaya dahil edilen Gram-pozitif bakteriler üzerinde farklı oranlarda etkili olup özellikle metanol ekstraktının diğerlerine oranla yüksek aktiviteye (15,3 mm-19,7 mm) sahip olduğunu göstermiştir. Kayma hareketi (swarming motility) üzerine ise metanol ve su ekstraktının inhibisyon etkisi %80 olarak bulunmuş, kloroform ekstraktı ise benzer bir sonuç ile %74 oranında önemli inhibisyon göstermiştir. Sonuç: Işkın bitkisi kök ekstraktlarının özellikle Gram pozitif bakteriler üzerinde antibakteriyel özelliğe sahip olduğu, P. aeruginosa PA01’in yaptığı kayma hareketini inhibe ettiği ve enfeksiyon hastalıkları ile mücadelede yeni nesil ilaçlara model olabilecek bir bitki olma potansiyeline sahip olabileceği düşünülmektedir.

Anahtar Kelimeler:

Rheum ribes L. kayma hareketi, çevreyi algılama, PA01, antibakteriyel

The Effect of Rheum ribes L. Extracts on Bacterial Communication and Antibacterial Activity

Objective: The discovery of quorum sensing systems regulating bacterial virulence has afforded a novel opportunity to control infections without interfering with growth. As many bacteria Pseudomonas aeruginosa also used this system synthesized of some virulence factor such as alkaline protease, pyocyanin, phospholipase and exotoxin A, biofilm formation and swarming motility. Swarming motility is one of the major virulence factors; it is known to play a role in early biofilm development. Material-Method: In this study, inhibitory effect of methanol (MeOH), methanol-chloroform (MeOH-CHCl3) and water (H2O) extracts of the Rheum ribes L. on swarming motility in P. aeruginosa PA01 was investigated. And also antibacterial activity and minimum inhibitory concentration (MIC) values were investigated on Gram-positive Bacillus cereus ATCC 11778, Enterecoccus faecalis ATCC 29212, Listeria monocytogenes ATCC 7644, Staphylococcus aureus ATCC 25923, Metisilin-Resistant Staphylococcus aureus ATCC 43300 and Gram-negative Chromobacterium violaceum ATCC 12472, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Pseudomonas aeruginosa PA01. Results: As a result, all extracts showed antibacterial effect on Gram-positive bacteria in different rate and according to swarming motility experiments, the highest inhibitory effect was seen in methanol and water extracts of Rheum ribes with 81%. Also chloroform extract showed similar inhibition rate (78%) on swarming motility. Conclusion: It is thought that the root extract of the plant has antibacterial properties especially on Gram positive bacteria, inhibits the swarming motility in P. aeruginosa PA01 and may have the potential to be a model for new generation drugs in the fight against infectious diseases.

Keywords:

Rheum ribes L. swarming motility, quorum sensing, PA01, antibacterial,

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