Gülsüm ABUŞOĞLU, Cengiz KOÇAK, Fatma KOÇAK, Bahadir OZTURK, Hüsamettin VATANSEV

CDDO-Me'nin Meme Kanseri Hücrelerindeki Etkilerinin Tamoxifen ve Docetaxel ile Karşılaştırılması

Amaç: Oleanolik asitten türetilen sentetik triterpenoidlerin, güçlü antiproliferatif ve antitümörojenik aktiviteye sahip oldukları bilinmektedir. Bu çalışmada, bir triterpenoid olan CDDO-Me'nin insan meme kanseri hücre hatları üzerindeki sitotoksik etkilerini, meme kanserinin rutin tedavisinde kullanılan Tamoxifen ve Docetaxel ile karşılaştırarak araştırmaktır. Materyal Metod: CDDO-Me, Docetaxel veTamoxifen’in sitotoksik etkilerini incelemek için meme kanseri hücreleri olarak MCF-7 ve MDA MB-231 hücre hatları tercih edildi. Her bir ilacın çeşitli dozları hücrelere uygulanarak sitotoksik etkileri xCELLigence cihazı ile belirlendi ve ilaçların IC50 değerleri belirlendi. IC50 dozlarıyla muamele edilen hücrelerden hücre blokları hazırlanarak, histolojik ve immünohistokimyasal boyama ile proliferasyon indeksi Ki-67 ve Cyclin D1, antiapoptotik Bcl-2 ve proapoptotik Bax protein ekspresyonları skorlandı. Bulgular: CDDO-Me, Docetaxel veTamoxifen her iki hücre hattında hücre canlılığını istatistiksel olarak önemli bir şekilde inhibe etti. CDDO-Me'nin Tamoxifen ve Docetaxel ile etkileri moleküler düzeyde karşılaştırıldığında, her iki hücre hattında da apopitoz yolağı açısından CDDO-Me’nin, Tamoxifen ve Docetaxel’e göre istatistiksel olarak daha etkili olduğu (p<0.001) sonucuna varıldı. Hücre döngüsü açısından ise MCF-7 hücrelerinde CDDO-Me'nin Tamoxifen ile istatistiksel olarak benzer etkiler gösterdiği ve Docetaxel’in bu hücre hattında istatistiksel olarak daha etkin olduğu (p<0.001) ve MDA MB-231 hücrelerinde ise CDDO-Me'nin Docetaxel ile istatistiksel olarak benzer etkiler gösterdiği ve Tamoxifenin bu hücre hattında istatistiksel olarak daha etkin olduğu gözlemlendi (p<0.001). Sonuç: CDDO-Me'nin antiproliferatif ve apopitoz indükleyici etkileri, rutin meme kanseri tedavisinde kullanılan Tamoxifen ve Docetaxel'in etkilerine göre her iki meme kanseri tipinde farklılık gösterse de, CDDO-Me, meme kanseri tedavisinde alternatif bir kemoterapötik ajan olabilir. Ayrıca bu çalışmanın sonuçları gelecekte yapılacak in vivo çalışmalara yol gösterici olabilir.

Anahtar Kelimeler:

Apopitoz, CDDO-Me, Docetaxel, meme kanseri, Tamoxifen

Comparison of the Effects of CDDO-Me with Tamoxifen and Docetaxel on Breast Cancer Cells

Keywords:

Apoptosis, breast cancer, CDDO-Me, Docetaxel, Tamoxifen,

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